Experiment: MS_media with m-arsenite 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_1894 and EX28DRAFT_1895 are separated by 21 nucleotides EX28DRAFT_1895 and EX28DRAFT_1896 are separated by 84 nucleotides EX28DRAFT_1896 and EX28DRAFT_1897 are separated by 232 nucleotides EX28DRAFT_1897 and EX28DRAFT_1898 are separated by 45 nucleotides EX28DRAFT_1898 and EX28DRAFT_1899 are separated by 48 nucleotides
EX28DRAFT_1894: EX28DRAFT_1894 - Uncharacterized component of anaerobic dehydrogenases, at 1,903,771 to 1,904,322
_1894
EX28DRAFT_1895: EX28DRAFT_1895 - Histidinol phosphatase and related hydrolases of the PHP family, at 1,904,344 to 1,905,081
_1895
EX28DRAFT_1896: EX28DRAFT_1896 - Phosphoglycerate dehydrogenase and related dehydrogenases, at 1,905,166 to 1,906,104
_1896
EX28DRAFT_1897: EX28DRAFT_1897 - tRNA, at 1,906,337 to 1,906,424
_1897
EX28DRAFT_1898: EX28DRAFT_1898 - RNA, at 1,906,470 to 1,906,607
_1898
EX28DRAFT_1899: EX28DRAFT_1899 - Transcriptional regulator, at 1,906,656 to 1,907,561
_1899
Position (kb)
1905
1906
1907 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 1904.234 kb on - strand, within EX28DRAFT_1894 at 1904.279 kb on + strand at 1904.279 kb on + strand at 1904.280 kb on - strand at 1904.345 kb on + strand at 1904.416 kb on + strand at 1904.446 kb on - strand, within EX28DRAFT_1895 at 1904.446 kb on - strand, within EX28DRAFT_1895 at 1904.608 kb on - strand, within EX28DRAFT_1895 at 1904.608 kb on - strand, within EX28DRAFT_1895 at 1904.985 kb on + strand, within EX28DRAFT_1895 at 1904.985 kb on + strand, within EX28DRAFT_1895 at 1904.994 kb on + strand, within EX28DRAFT_1895 at 1905.014 kb on + strand at 1905.015 kb on - strand at 1905.015 kb on - strand at 1905.032 kb on + strand at 1905.032 kb on + strand at 1905.061 kb on - strand at 1905.061 kb on - strand at 1905.077 kb on + strand at 1905.136 kb on - strand at 1905.238 kb on + strand at 1905.239 kb on - strand at 1905.240 kb on + strand at 1905.240 kb on + strand at 1905.241 kb on - strand at 1905.241 kb on - strand at 1905.241 kb on - strand at 1905.241 kb on - strand at 1905.241 kb on - strand at 1905.241 kb on - strand at 1905.242 kb on + strand at 1905.242 kb on + strand at 1905.242 kb on + strand at 1905.242 kb on + strand at 1905.242 kb on + strand at 1905.242 kb on + strand at 1905.243 kb on - strand at 1905.346 kb on - strand, within EX28DRAFT_1896 at 1905.347 kb on + strand, within EX28DRAFT_1896 at 1905.348 kb on - strand, within EX28DRAFT_1896 at 1905.348 kb on - strand, within EX28DRAFT_1896 at 1905.443 kb on + strand, within EX28DRAFT_1896 at 1905.523 kb on - strand, within EX28DRAFT_1896 at 1905.693 kb on + strand, within EX28DRAFT_1896 at 1905.784 kb on + strand, within EX28DRAFT_1896 at 1905.800 kb on + strand, within EX28DRAFT_1896 at 1905.800 kb on + strand, within EX28DRAFT_1896 at 1905.983 kb on + strand, within EX28DRAFT_1896 at 1905.984 kb on - strand, within EX28DRAFT_1896 at 1906.064 kb on + strand at 1906.088 kb on + strand at 1906.096 kb on + strand at 1906.097 kb on - strand at 1906.101 kb on - strand at 1906.172 kb on + strand at 1906.218 kb on + strand at 1906.218 kb on + strand at 1906.254 kb on + strand at 1906.297 kb on + strand at 1906.429 kb on + strand at 1906.429 kb on + strand at 1906.429 kb on + strand at 1906.429 kb on + strand at 1906.604 kb on + strand at 1906.604 kb on + strand at 1906.604 kb on + strand at 1906.604 kb on + strand at 1906.604 kb on + strand at 1906.604 kb on + strand at 1906.606 kb on + strand at 1906.606 kb on + strand at 1906.607 kb on - strand at 1906.678 kb on + strand at 1906.958 kb on + strand, within EX28DRAFT_1899 at 1906.959 kb on - strand, within EX28DRAFT_1899 at 1906.964 kb on + strand, within EX28DRAFT_1899 at 1906.964 kb on + strand, within EX28DRAFT_1899 at 1906.965 kb on - strand, within EX28DRAFT_1899 at 1907.092 kb on - strand, within EX28DRAFT_1899 at 1907.095 kb on - strand, within EX28DRAFT_1899
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media with m-arsenite 1 mM remove 1,904,234 - EX28DRAFT_1894 0.84 +0.2 1,904,279 + +0.3 1,904,279 + +0.2 1,904,280 - -0.1 1,904,345 + -0.3 1,904,416 + -0.4 1,904,446 - EX28DRAFT_1895 0.14 +0.4 1,904,446 - EX28DRAFT_1895 0.14 -0.5 1,904,608 - EX28DRAFT_1895 0.36 +0.7 1,904,608 - EX28DRAFT_1895 0.36 +0.7 1,904,985 + EX28DRAFT_1895 0.87 -2.6 1,904,985 + EX28DRAFT_1895 0.87 +0.2 1,904,994 + EX28DRAFT_1895 0.88 -1.8 1,905,014 + -0.6 1,905,015 - +0.7 1,905,015 - -0.3 1,905,032 + +0.1 1,905,032 + -0.2 1,905,061 - -0.1 1,905,061 - -0.8 1,905,077 + +0.4 1,905,136 - -0.1 1,905,238 + -0.4 1,905,239 - -0.5 1,905,240 + -0.1 1,905,240 + -2.4 1,905,241 - +0.4 1,905,241 - +0.2 1,905,241 - +0.4 1,905,241 - -0.5 1,905,241 - -0.1 1,905,241 - +0.3 1,905,242 + +0.7 1,905,242 + +0.5 1,905,242 + +0.1 1,905,242 + +1.0 1,905,242 + -1.3 1,905,242 + +0.2 1,905,243 - -0.7 1,905,346 - EX28DRAFT_1896 0.19 +0.2 1,905,347 + EX28DRAFT_1896 0.19 -0.7 1,905,348 - EX28DRAFT_1896 0.19 +0.1 1,905,348 - EX28DRAFT_1896 0.19 +0.2 1,905,443 + EX28DRAFT_1896 0.29 +1.4 1,905,523 - EX28DRAFT_1896 0.38 +0.2 1,905,693 + EX28DRAFT_1896 0.56 +0.2 1,905,784 + EX28DRAFT_1896 0.66 -0.6 1,905,800 + EX28DRAFT_1896 0.68 +0.8 1,905,800 + EX28DRAFT_1896 0.68 +0.5 1,905,983 + EX28DRAFT_1896 0.87 +0.2 1,905,984 - EX28DRAFT_1896 0.87 +1.0 1,906,064 + +0.6 1,906,088 + -4.2 1,906,096 + +1.1 1,906,097 - +0.1 1,906,101 - -0.0 1,906,172 + -0.9 1,906,218 + +0.8 1,906,218 + -0.1 1,906,254 + +0.3 1,906,297 + -0.7 1,906,429 + +0.6 1,906,429 + -0.6 1,906,429 + +0.6 1,906,429 + -0.5 1,906,604 + +1.6 1,906,604 + -0.2 1,906,604 + -0.5 1,906,604 + +1.7 1,906,604 + -0.5 1,906,604 + +1.4 1,906,606 + +0.2 1,906,606 + -0.7 1,906,607 - -5.7 1,906,678 + +0.4 1,906,958 + EX28DRAFT_1899 0.33 -0.5 1,906,959 - EX28DRAFT_1899 0.33 -0.2 1,906,964 + EX28DRAFT_1899 0.34 -0.9 1,906,964 + EX28DRAFT_1899 0.34 -0.5 1,906,965 - EX28DRAFT_1899 0.34 +0.6 1,907,092 - EX28DRAFT_1899 0.48 -1.1 1,907,095 - EX28DRAFT_1899 0.48 +0.1
Or see this region's nucleotide sequence