Experiment: MS_media with m-arsenite 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_1317 and EX28DRAFT_1318 are separated by 69 nucleotides EX28DRAFT_1318 and EX28DRAFT_1319 overlap by 7 nucleotides EX28DRAFT_1319 and EX28DRAFT_1320 overlap by 17 nucleotides EX28DRAFT_1320 and EX28DRAFT_1321 are separated by 3 nucleotides EX28DRAFT_1321 and EX28DRAFT_1322 are separated by 11 nucleotides
EX28DRAFT_1317: EX28DRAFT_1317 - Protein of unknown function (DUF1482), at 1,319,739 to 1,319,936
_1317
EX28DRAFT_1318: EX28DRAFT_1318 - hypothetical protein, at 1,320,006 to 1,320,401
_1318
EX28DRAFT_1319: EX28DRAFT_1319 - hypothetical protein, at 1,320,395 to 1,320,550
_1319
EX28DRAFT_1320: EX28DRAFT_1320 - DNA polymerase III, epsilon subunit and related 3'-5' exonucleases, at 1,320,534 to 1,321,190
_1320
EX28DRAFT_1321: EX28DRAFT_1321 - hypothetical protein, at 1,321,194 to 1,321,859
_1321
EX28DRAFT_1322: EX28DRAFT_1322 - hypothetical protein, at 1,321,871 to 1,322,581
_1322
Position (kb)
1320
1321
1322 Strain fitness (log2 ratio)
-2
-1
0
1 at 1319.569 kb on + strand at 1319.569 kb on + strand at 1319.570 kb on - strand at 1319.570 kb on - strand at 1319.570 kb on - strand at 1319.570 kb on - strand at 1319.609 kb on + strand at 1319.967 kb on - strand at 1320.150 kb on + strand, within EX28DRAFT_1318 at 1320.151 kb on - strand, within EX28DRAFT_1318 at 1320.151 kb on - strand, within EX28DRAFT_1318 at 1320.241 kb on - strand, within EX28DRAFT_1318 at 1320.294 kb on + strand, within EX28DRAFT_1318 at 1320.295 kb on - strand, within EX28DRAFT_1318 at 1320.618 kb on - strand, within EX28DRAFT_1320 at 1320.618 kb on - strand, within EX28DRAFT_1320 at 1320.689 kb on + strand, within EX28DRAFT_1320 at 1320.690 kb on - strand, within EX28DRAFT_1320 at 1320.701 kb on + strand, within EX28DRAFT_1320 at 1320.702 kb on - strand, within EX28DRAFT_1320 at 1320.744 kb on + strand, within EX28DRAFT_1320 at 1320.763 kb on - strand, within EX28DRAFT_1320 at 1320.882 kb on + strand, within EX28DRAFT_1320 at 1320.958 kb on - strand, within EX28DRAFT_1320 at 1320.982 kb on - strand, within EX28DRAFT_1320 at 1321.074 kb on - strand, within EX28DRAFT_1320 at 1321.188 kb on + strand at 1321.242 kb on + strand at 1321.243 kb on - strand at 1321.275 kb on - strand, within EX28DRAFT_1321 at 1321.444 kb on + strand, within EX28DRAFT_1321 at 1321.445 kb on - strand, within EX28DRAFT_1321 at 1321.448 kb on + strand, within EX28DRAFT_1321 at 1321.448 kb on + strand, within EX28DRAFT_1321 at 1321.449 kb on - strand, within EX28DRAFT_1321 at 1321.449 kb on - strand, within EX28DRAFT_1321 at 1321.449 kb on - strand, within EX28DRAFT_1321 at 1321.449 kb on - strand, within EX28DRAFT_1321 at 1321.512 kb on + strand, within EX28DRAFT_1321 at 1321.555 kb on + strand, within EX28DRAFT_1321 at 1321.556 kb on - strand, within EX28DRAFT_1321 at 1321.556 kb on - strand, within EX28DRAFT_1321 at 1321.574 kb on - strand, within EX28DRAFT_1321 at 1321.574 kb on - strand, within EX28DRAFT_1321 at 1321.574 kb on - strand, within EX28DRAFT_1321 at 1321.594 kb on - strand, within EX28DRAFT_1321 at 1321.857 kb on + strand at 1321.857 kb on + strand at 1321.858 kb on - strand at 1321.892 kb on - strand at 1321.965 kb on - strand, within EX28DRAFT_1322 at 1321.965 kb on - strand, within EX28DRAFT_1322 at 1321.991 kb on + strand, within EX28DRAFT_1322 at 1321.991 kb on + strand, within EX28DRAFT_1322 at 1321.992 kb on - strand, within EX28DRAFT_1322
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media with m-arsenite 1 mM remove 1,319,569 + +1.5 1,319,569 + +0.9 1,319,570 - +0.1 1,319,570 - -0.8 1,319,570 - -2.3 1,319,570 - +0.2 1,319,609 + -0.9 1,319,967 - +0.2 1,320,150 + EX28DRAFT_1318 0.36 -0.4 1,320,151 - EX28DRAFT_1318 0.37 -0.2 1,320,151 - EX28DRAFT_1318 0.37 +0.7 1,320,241 - EX28DRAFT_1318 0.59 -0.3 1,320,294 + EX28DRAFT_1318 0.73 -0.1 1,320,295 - EX28DRAFT_1318 0.73 +0.1 1,320,618 - EX28DRAFT_1320 0.13 -0.1 1,320,618 - EX28DRAFT_1320 0.13 +1.0 1,320,689 + EX28DRAFT_1320 0.24 +1.3 1,320,690 - EX28DRAFT_1320 0.24 -2.0 1,320,701 + EX28DRAFT_1320 0.25 +0.7 1,320,702 - EX28DRAFT_1320 0.26 +0.5 1,320,744 + EX28DRAFT_1320 0.32 -0.6 1,320,763 - EX28DRAFT_1320 0.35 +0.1 1,320,882 + EX28DRAFT_1320 0.53 -0.3 1,320,958 - EX28DRAFT_1320 0.65 +0.3 1,320,982 - EX28DRAFT_1320 0.68 -0.1 1,321,074 - EX28DRAFT_1320 0.82 +0.2 1,321,188 + +1.0 1,321,242 + -0.0 1,321,243 - -0.4 1,321,275 - EX28DRAFT_1321 0.12 -0.3 1,321,444 + EX28DRAFT_1321 0.38 +0.0 1,321,445 - EX28DRAFT_1321 0.38 +0.0 1,321,448 + EX28DRAFT_1321 0.38 +0.8 1,321,448 + EX28DRAFT_1321 0.38 +0.2 1,321,449 - EX28DRAFT_1321 0.38 -1.2 1,321,449 - EX28DRAFT_1321 0.38 +0.6 1,321,449 - EX28DRAFT_1321 0.38 -0.9 1,321,449 - EX28DRAFT_1321 0.38 -0.7 1,321,512 + EX28DRAFT_1321 0.48 +0.8 1,321,555 + EX28DRAFT_1321 0.54 +0.5 1,321,556 - EX28DRAFT_1321 0.54 -2.2 1,321,556 - EX28DRAFT_1321 0.54 -0.2 1,321,574 - EX28DRAFT_1321 0.57 +0.3 1,321,574 - EX28DRAFT_1321 0.57 +0.2 1,321,574 - EX28DRAFT_1321 0.57 +0.5 1,321,594 - EX28DRAFT_1321 0.60 -1.2 1,321,857 + -0.2 1,321,857 + -0.5 1,321,858 - +0.4 1,321,892 - -0.2 1,321,965 - EX28DRAFT_1322 0.13 +0.5 1,321,965 - EX28DRAFT_1322 0.13 +0.7 1,321,991 + EX28DRAFT_1322 0.17 +0.3 1,321,991 + EX28DRAFT_1322 0.17 -0.4 1,321,992 - EX28DRAFT_1322 0.17 -0.1
Or see this region's nucleotide sequence