Experiment: MS_media with m-arsenite 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_1216 and EX28DRAFT_1217 are separated by 156 nucleotides EX28DRAFT_1217 and EX28DRAFT_1218 are separated by 61 nucleotides EX28DRAFT_1218 and EX28DRAFT_1219 are separated by 40 nucleotides EX28DRAFT_1219 and EX28DRAFT_1220 are separated by 163 nucleotides
EX28DRAFT_1216: EX28DRAFT_1216 - Methionine synthase II (cobalamin-independent), at 1,234,941 to 1,236,044
_1216
EX28DRAFT_1217: EX28DRAFT_1217 - Rhodanese-related sulfurtransferase, at 1,236,201 to 1,236,614
_1217
EX28DRAFT_1218: EX28DRAFT_1218 - Transcriptional regulator containing an amidase domain and an AraC-type DNA-binding HTH domain, at 1,236,676 to 1,237,695
_1218
EX28DRAFT_1219: EX28DRAFT_1219 - Dihydrofolate reductase, at 1,237,736 to 1,238,272
_1219
EX28DRAFT_1220: EX28DRAFT_1220 - hypothetical protein, at 1,238,436 to 1,238,798
_1220
Position (kb)
1236
1237
1238 Strain fitness (log2 ratio)
-2
-1
0
1 at 1236.123 kb on - strand at 1236.123 kb on - strand at 1236.144 kb on + strand at 1236.352 kb on + strand, within EX28DRAFT_1217 at 1236.436 kb on - strand, within EX28DRAFT_1217 at 1236.478 kb on - strand, within EX28DRAFT_1217 at 1236.602 kb on + strand at 1236.607 kb on + strand at 1236.649 kb on + strand at 1236.649 kb on + strand at 1236.649 kb on + strand at 1236.650 kb on - strand at 1236.650 kb on - strand at 1236.771 kb on + strand at 1236.918 kb on - strand, within EX28DRAFT_1218 at 1237.128 kb on - strand, within EX28DRAFT_1218 at 1237.207 kb on - strand, within EX28DRAFT_1218 at 1237.713 kb on + strand at 1237.713 kb on + strand at 1237.779 kb on - strand at 1237.848 kb on - strand, within EX28DRAFT_1219 at 1237.908 kb on + strand, within EX28DRAFT_1219 at 1237.931 kb on + strand, within EX28DRAFT_1219 at 1238.061 kb on + strand, within EX28DRAFT_1219 at 1238.061 kb on + strand, within EX28DRAFT_1219 at 1238.061 kb on + strand, within EX28DRAFT_1219 at 1238.061 kb on + strand, within EX28DRAFT_1219 at 1238.062 kb on - strand, within EX28DRAFT_1219 at 1238.062 kb on - strand, within EX28DRAFT_1219 at 1238.062 kb on - strand, within EX28DRAFT_1219 at 1238.152 kb on - strand, within EX28DRAFT_1219 at 1238.152 kb on - strand, within EX28DRAFT_1219 at 1238.414 kb on + strand at 1238.415 kb on - strand at 1238.415 kb on - strand at 1238.425 kb on - strand at 1238.465 kb on - strand at 1238.472 kb on - strand at 1238.543 kb on - strand, within EX28DRAFT_1220 at 1238.563 kb on + strand, within EX28DRAFT_1220 at 1238.564 kb on - strand, within EX28DRAFT_1220 at 1238.674 kb on - strand, within EX28DRAFT_1220
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media with m-arsenite 1 mM remove 1,236,123 - +0.2 1,236,123 - +0.4 1,236,144 + +0.3 1,236,352 + EX28DRAFT_1217 0.36 -0.5 1,236,436 - EX28DRAFT_1217 0.57 -0.5 1,236,478 - EX28DRAFT_1217 0.67 +0.6 1,236,602 + -0.9 1,236,607 + +0.7 1,236,649 + -0.8 1,236,649 + +0.0 1,236,649 + +0.8 1,236,650 - +0.2 1,236,650 - -0.1 1,236,771 + +0.3 1,236,918 - EX28DRAFT_1218 0.24 +1.5 1,237,128 - EX28DRAFT_1218 0.44 -0.6 1,237,207 - EX28DRAFT_1218 0.52 +0.2 1,237,713 + +0.7 1,237,713 + +1.3 1,237,779 - -0.3 1,237,848 - EX28DRAFT_1219 0.21 +0.8 1,237,908 + EX28DRAFT_1219 0.32 +0.6 1,237,931 + EX28DRAFT_1219 0.36 -0.4 1,238,061 + EX28DRAFT_1219 0.61 +0.7 1,238,061 + EX28DRAFT_1219 0.61 -0.6 1,238,061 + EX28DRAFT_1219 0.61 -1.1 1,238,061 + EX28DRAFT_1219 0.61 +0.3 1,238,062 - EX28DRAFT_1219 0.61 +0.5 1,238,062 - EX28DRAFT_1219 0.61 -0.3 1,238,062 - EX28DRAFT_1219 0.61 -2.2 1,238,152 - EX28DRAFT_1219 0.77 +1.0 1,238,152 - EX28DRAFT_1219 0.77 -0.1 1,238,414 + +0.3 1,238,415 - +0.5 1,238,415 - -0.5 1,238,425 - +0.4 1,238,465 - -1.0 1,238,472 - +0.1 1,238,543 - EX28DRAFT_1220 0.29 -2.1 1,238,563 + EX28DRAFT_1220 0.35 -1.9 1,238,564 - EX28DRAFT_1220 0.35 -0.0 1,238,674 - EX28DRAFT_1220 0.66 -0.1
Or see this region's nucleotide sequence