Experiment: MS_media with m-arsenite 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0653 and EX28DRAFT_0654 are separated by 14 nucleotides EX28DRAFT_0654 and EX28DRAFT_0655 are separated by 176 nucleotides EX28DRAFT_0655 and EX28DRAFT_0656 overlap by 26 nucleotides
EX28DRAFT_0653: EX28DRAFT_0653 - ABC-type sugar transport system, ATPase component, at 647,019 to 648,533
_0653
EX28DRAFT_0654: EX28DRAFT_0654 - Ribose/xylose/arabinose/galactoside ABC-type transport systems, permease components, at 648,548 to 649,534
_0654
EX28DRAFT_0655: EX28DRAFT_0655 - trehalose-phosphatase, at 649,711 to 650,514
_0655
EX28DRAFT_0656: EX28DRAFT_0656 - alpha,alpha-trehalose-phosphate synthase [UDP-forming], at 650,489 to 651,913
_0656
Position (kb)
648
649
650 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 647.635 kb on + strand, within EX28DRAFT_0653 at 647.636 kb on - strand, within EX28DRAFT_0653 at 647.636 kb on - strand, within EX28DRAFT_0653 at 647.763 kb on + strand, within EX28DRAFT_0653 at 647.764 kb on - strand, within EX28DRAFT_0653 at 647.960 kb on + strand, within EX28DRAFT_0653 at 648.038 kb on + strand, within EX28DRAFT_0653 at 648.085 kb on + strand, within EX28DRAFT_0653 at 648.087 kb on - strand, within EX28DRAFT_0653 at 648.183 kb on - strand, within EX28DRAFT_0653 at 648.247 kb on + strand, within EX28DRAFT_0653 at 648.269 kb on + strand, within EX28DRAFT_0653 at 648.269 kb on + strand, within EX28DRAFT_0653 at 648.269 kb on + strand, within EX28DRAFT_0653 at 648.333 kb on + strand, within EX28DRAFT_0653 at 648.333 kb on + strand, within EX28DRAFT_0653 at 648.334 kb on - strand, within EX28DRAFT_0653 at 648.335 kb on + strand, within EX28DRAFT_0653 at 648.335 kb on + strand, within EX28DRAFT_0653 at 648.345 kb on + strand, within EX28DRAFT_0653 at 648.345 kb on + strand, within EX28DRAFT_0653 at 648.345 kb on + strand, within EX28DRAFT_0653 at 648.346 kb on - strand, within EX28DRAFT_0653 at 648.535 kb on + strand at 648.535 kb on + strand at 648.536 kb on - strand at 648.544 kb on - strand at 648.544 kb on - strand at 648.560 kb on - strand at 648.562 kb on + strand at 648.607 kb on + strand at 648.607 kb on + strand at 648.694 kb on + strand, within EX28DRAFT_0654 at 648.697 kb on + strand, within EX28DRAFT_0654 at 648.698 kb on - strand, within EX28DRAFT_0654 at 648.698 kb on - strand, within EX28DRAFT_0654 at 648.986 kb on + strand, within EX28DRAFT_0654 at 648.988 kb on + strand, within EX28DRAFT_0654 at 648.988 kb on + strand, within EX28DRAFT_0654 at 648.989 kb on - strand, within EX28DRAFT_0654 at 648.989 kb on - strand, within EX28DRAFT_0654 at 649.145 kb on - strand, within EX28DRAFT_0654 at 649.213 kb on + strand, within EX28DRAFT_0654 at 649.258 kb on + strand, within EX28DRAFT_0654 at 649.259 kb on - strand, within EX28DRAFT_0654 at 649.259 kb on - strand, within EX28DRAFT_0654 at 649.379 kb on + strand, within EX28DRAFT_0654 at 649.380 kb on - strand, within EX28DRAFT_0654 at 649.380 kb on - strand, within EX28DRAFT_0654 at 649.457 kb on + strand at 649.565 kb on - strand at 649.572 kb on + strand at 649.572 kb on + strand at 649.572 kb on + strand at 649.647 kb on - strand at 649.649 kb on - strand at 649.649 kb on - strand at 649.652 kb on + strand at 649.652 kb on + strand at 649.652 kb on + strand at 649.652 kb on + strand at 649.753 kb on + strand at 649.754 kb on - strand at 649.754 kb on - strand at 649.819 kb on - strand, within EX28DRAFT_0655 at 649.992 kb on + strand, within EX28DRAFT_0655 at 650.213 kb on + strand, within EX28DRAFT_0655 at 650.214 kb on - strand, within EX28DRAFT_0655 at 650.232 kb on + strand, within EX28DRAFT_0655 at 650.430 kb on - strand, within EX28DRAFT_0655 at 650.462 kb on + strand at 650.463 kb on - strand at 650.499 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media with m-arsenite 1 mM remove 647,635 + EX28DRAFT_0653 0.41 -0.9 647,636 - EX28DRAFT_0653 0.41 +0.3 647,636 - EX28DRAFT_0653 0.41 +0.5 647,763 + EX28DRAFT_0653 0.49 -0.2 647,764 - EX28DRAFT_0653 0.49 +0.6 647,960 + EX28DRAFT_0653 0.62 -0.3 648,038 + EX28DRAFT_0653 0.67 -1.5 648,085 + EX28DRAFT_0653 0.70 -0.6 648,087 - EX28DRAFT_0653 0.70 -0.2 648,183 - EX28DRAFT_0653 0.77 -0.2 648,247 + EX28DRAFT_0653 0.81 +0.1 648,269 + EX28DRAFT_0653 0.83 +0.4 648,269 + EX28DRAFT_0653 0.83 +0.2 648,269 + EX28DRAFT_0653 0.83 -0.7 648,333 + EX28DRAFT_0653 0.87 -0.4 648,333 + EX28DRAFT_0653 0.87 -0.2 648,334 - EX28DRAFT_0653 0.87 +1.0 648,335 + EX28DRAFT_0653 0.87 -0.6 648,335 + EX28DRAFT_0653 0.87 -2.9 648,345 + EX28DRAFT_0653 0.88 +0.7 648,345 + EX28DRAFT_0653 0.88 +2.5 648,345 + EX28DRAFT_0653 0.88 +0.2 648,346 - EX28DRAFT_0653 0.88 -0.3 648,535 + +0.5 648,535 + +0.4 648,536 - -0.6 648,544 - -0.7 648,544 - +1.0 648,560 - +0.4 648,562 + +0.8 648,607 + -2.9 648,607 + +0.2 648,694 + EX28DRAFT_0654 0.15 +0.5 648,697 + EX28DRAFT_0654 0.15 -0.8 648,698 - EX28DRAFT_0654 0.15 -0.3 648,698 - EX28DRAFT_0654 0.15 -0.3 648,986 + EX28DRAFT_0654 0.44 -0.6 648,988 + EX28DRAFT_0654 0.45 -0.3 648,988 + EX28DRAFT_0654 0.45 -1.1 648,989 - EX28DRAFT_0654 0.45 -0.2 648,989 - EX28DRAFT_0654 0.45 +1.7 649,145 - EX28DRAFT_0654 0.60 -0.3 649,213 + EX28DRAFT_0654 0.67 -0.9 649,258 + EX28DRAFT_0654 0.72 -0.0 649,259 - EX28DRAFT_0654 0.72 +0.0 649,259 - EX28DRAFT_0654 0.72 -0.0 649,379 + EX28DRAFT_0654 0.84 -0.2 649,380 - EX28DRAFT_0654 0.84 +0.4 649,380 - EX28DRAFT_0654 0.84 -0.7 649,457 + +0.2 649,565 - +1.2 649,572 + -3.2 649,572 + +0.8 649,572 + -0.1 649,647 - -0.4 649,649 - +0.9 649,649 - +0.6 649,652 + +0.2 649,652 + -2.3 649,652 + +0.0 649,652 + -0.0 649,753 + +0.1 649,754 - +0.1 649,754 - +0.6 649,819 - EX28DRAFT_0655 0.13 +0.3 649,992 + EX28DRAFT_0655 0.35 +0.1 650,213 + EX28DRAFT_0655 0.62 -0.5 650,214 - EX28DRAFT_0655 0.63 -0.4 650,232 + EX28DRAFT_0655 0.65 -0.5 650,430 - EX28DRAFT_0655 0.89 +0.6 650,462 + +0.5 650,463 - -0.1 650,499 + -0.4
Or see this region's nucleotide sequence