Experiment: MS_media with m-arsenite 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0538 and EX28DRAFT_0539 overlap by 4 nucleotides EX28DRAFT_0539 and EX28DRAFT_0540 are separated by 5 nucleotides EX28DRAFT_0540 and EX28DRAFT_0541 are separated by 45 nucleotides
EX28DRAFT_0538: EX28DRAFT_0538 - histidinol-phosphate aminotransferase, at 531,843 to 532,904
_0538
EX28DRAFT_0539: EX28DRAFT_0539 - histidinol dehydrogenase, at 532,901 to 534,205
_0539
EX28DRAFT_0540: EX28DRAFT_0540 - ATP phosphoribosyltransferase, at 534,211 to 535,110
_0540
EX28DRAFT_0541: EX28DRAFT_0541 - RNA, at 535,156 to 535,279
_0541
Position (kb) 532
533
534
535 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2 at 531.906 kb on - strand at 531.939 kb on - strand at 531.956 kb on + strand, within EX28DRAFT_0538 at 532.018 kb on + strand, within EX28DRAFT_0538 at 532.019 kb on - strand, within EX28DRAFT_0538 at 532.022 kb on + strand, within EX28DRAFT_0538 at 532.023 kb on - strand, within EX28DRAFT_0538 at 532.082 kb on - strand, within EX28DRAFT_0538 at 532.355 kb on - strand, within EX28DRAFT_0538 at 532.576 kb on + strand, within EX28DRAFT_0538 at 532.589 kb on + strand, within EX28DRAFT_0538 at 532.590 kb on - strand, within EX28DRAFT_0538 at 532.590 kb on - strand, within EX28DRAFT_0538 at 532.600 kb on + strand, within EX28DRAFT_0538 at 532.705 kb on + strand, within EX28DRAFT_0538 at 532.856 kb on - strand at 533.179 kb on + strand, within EX28DRAFT_0539 at 533.569 kb on - strand, within EX28DRAFT_0539 at 533.569 kb on - strand, within EX28DRAFT_0539 at 533.659 kb on + strand, within EX28DRAFT_0539 at 533.659 kb on + strand, within EX28DRAFT_0539 at 533.659 kb on + strand, within EX28DRAFT_0539 at 533.660 kb on - strand, within EX28DRAFT_0539 at 533.660 kb on - strand, within EX28DRAFT_0539 at 533.660 kb on - strand, within EX28DRAFT_0539 at 533.661 kb on + strand, within EX28DRAFT_0539 at 533.662 kb on - strand, within EX28DRAFT_0539 at 533.662 kb on - strand, within EX28DRAFT_0539 at 533.817 kb on + strand, within EX28DRAFT_0539 at 533.818 kb on - strand, within EX28DRAFT_0539 at 533.818 kb on - strand, within EX28DRAFT_0539 at 533.917 kb on + strand, within EX28DRAFT_0539 at 533.918 kb on - strand, within EX28DRAFT_0539 at 534.045 kb on + strand, within EX28DRAFT_0539 at 534.389 kb on - strand, within EX28DRAFT_0540 at 534.389 kb on - strand, within EX28DRAFT_0540 at 534.389 kb on - strand, within EX28DRAFT_0540 at 534.389 kb on - strand, within EX28DRAFT_0540 at 534.428 kb on + strand, within EX28DRAFT_0540 at 534.428 kb on + strand, within EX28DRAFT_0540 at 534.429 kb on - strand, within EX28DRAFT_0540 at 534.545 kb on + strand, within EX28DRAFT_0540 at 534.732 kb on + strand, within EX28DRAFT_0540 at 534.733 kb on - strand, within EX28DRAFT_0540 at 534.733 kb on - strand, within EX28DRAFT_0540 at 534.834 kb on - strand, within EX28DRAFT_0540 at 534.939 kb on + strand, within EX28DRAFT_0540 at 535.015 kb on - strand, within EX28DRAFT_0540 at 535.081 kb on - strand at 535.081 kb on - strand at 535.084 kb on + strand at 535.084 kb on + strand at 535.085 kb on - strand at 535.085 kb on - strand at 535.135 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media with m-arsenite 1 mM remove 531,906 - -3.3 531,939 - +0.7 531,956 + EX28DRAFT_0538 0.11 -0.7 532,018 + EX28DRAFT_0538 0.16 -2.3 532,019 - EX28DRAFT_0538 0.17 -4.4 532,022 + EX28DRAFT_0538 0.17 -3.8 532,023 - EX28DRAFT_0538 0.17 -1.8 532,082 - EX28DRAFT_0538 0.23 +0.1 532,355 - EX28DRAFT_0538 0.48 -2.7 532,576 + EX28DRAFT_0538 0.69 -4.7 532,589 + EX28DRAFT_0538 0.70 -1.7 532,590 - EX28DRAFT_0538 0.70 -2.9 532,590 - EX28DRAFT_0538 0.70 -3.6 532,600 + EX28DRAFT_0538 0.71 -3.1 532,705 + EX28DRAFT_0538 0.81 -5.0 532,856 - -3.3 533,179 + EX28DRAFT_0539 0.21 -3.2 533,569 - EX28DRAFT_0539 0.51 +0.2 533,569 - EX28DRAFT_0539 0.51 -3.3 533,659 + EX28DRAFT_0539 0.58 -1.7 533,659 + EX28DRAFT_0539 0.58 -0.4 533,659 + EX28DRAFT_0539 0.58 -2.8 533,660 - EX28DRAFT_0539 0.58 +1.1 533,660 - EX28DRAFT_0539 0.58 -3.5 533,660 - EX28DRAFT_0539 0.58 -3.3 533,661 + EX28DRAFT_0539 0.58 +2.1 533,662 - EX28DRAFT_0539 0.58 -3.8 533,662 - EX28DRAFT_0539 0.58 -1.1 533,817 + EX28DRAFT_0539 0.70 -3.8 533,818 - EX28DRAFT_0539 0.70 -4.9 533,818 - EX28DRAFT_0539 0.70 -0.4 533,917 + EX28DRAFT_0539 0.78 -2.9 533,918 - EX28DRAFT_0539 0.78 -1.4 534,045 + EX28DRAFT_0539 0.88 -3.5 534,389 - EX28DRAFT_0540 0.20 -1.3 534,389 - EX28DRAFT_0540 0.20 -3.2 534,389 - EX28DRAFT_0540 0.20 -2.0 534,389 - EX28DRAFT_0540 0.20 -1.5 534,428 + EX28DRAFT_0540 0.24 -0.7 534,428 + EX28DRAFT_0540 0.24 -3.1 534,429 - EX28DRAFT_0540 0.24 +0.1 534,545 + EX28DRAFT_0540 0.37 -1.1 534,732 + EX28DRAFT_0540 0.58 -2.1 534,733 - EX28DRAFT_0540 0.58 -3.7 534,733 - EX28DRAFT_0540 0.58 -3.3 534,834 - EX28DRAFT_0540 0.69 -4.5 534,939 + EX28DRAFT_0540 0.81 +0.7 535,015 - EX28DRAFT_0540 0.89 -3.6 535,081 - +2.1 535,081 - -1.4 535,084 + -3.8 535,084 + -2.2 535,085 - -1.4 535,085 - -1.7 535,135 + -3.8
Or see this region's nucleotide sequence