Experiment: MS_media with m-arsenite 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0531 and EX28DRAFT_0532 are separated by 192 nucleotides EX28DRAFT_0532 and EX28DRAFT_0533 are separated by 40 nucleotides EX28DRAFT_0533 and EX28DRAFT_0534 overlap by 7 nucleotides
EX28DRAFT_0531: EX28DRAFT_0531 - Nucleoside-diphosphate-sugar epimerases, at 525,871 to 526,875
_0531
EX28DRAFT_0532: EX28DRAFT_0532 - Chain length determinant protein, at 527,068 to 528,048
_0532
EX28DRAFT_0533: EX28DRAFT_0533 - phosphoribosyl-AMP cyclohydrolase (EC 3.5.4.19)/phosphoribosyl-ATP pyrophosphatase (EC 3.6.1.31), at 528,089 to 528,700
_0533
EX28DRAFT_0534: EX28DRAFT_0534 - imidazoleglycerol phosphate synthase, cyclase subunit, at 528,694 to 529,470
_0534
Position (kb)
527
528
529 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 526.103 kb on + strand, within EX28DRAFT_0531 at 526.176 kb on + strand, within EX28DRAFT_0531 at 526.177 kb on - strand, within EX28DRAFT_0531 at 526.195 kb on - strand, within EX28DRAFT_0531 at 526.220 kb on + strand, within EX28DRAFT_0531 at 526.221 kb on - strand, within EX28DRAFT_0531 at 526.248 kb on - strand, within EX28DRAFT_0531 at 526.251 kb on + strand, within EX28DRAFT_0531 at 526.253 kb on + strand, within EX28DRAFT_0531 at 526.290 kb on + strand, within EX28DRAFT_0531 at 526.291 kb on - strand, within EX28DRAFT_0531 at 526.296 kb on + strand, within EX28DRAFT_0531 at 526.331 kb on + strand, within EX28DRAFT_0531 at 526.371 kb on - strand, within EX28DRAFT_0531 at 526.371 kb on - strand, within EX28DRAFT_0531 at 526.383 kb on - strand, within EX28DRAFT_0531 at 526.489 kb on + strand, within EX28DRAFT_0531 at 526.793 kb on - strand at 526.929 kb on + strand at 526.930 kb on - strand at 526.935 kb on - strand at 526.975 kb on + strand at 526.976 kb on - strand at 526.976 kb on - strand at 527.013 kb on + strand at 527.013 kb on + strand at 527.014 kb on - strand at 527.014 kb on - strand at 527.014 kb on - strand at 527.014 kb on - strand at 527.014 kb on - strand at 527.015 kb on + strand at 527.015 kb on + strand at 527.015 kb on + strand at 527.015 kb on + strand at 527.015 kb on + strand at 527.015 kb on + strand at 527.015 kb on + strand at 527.016 kb on - strand at 527.016 kb on - strand at 527.016 kb on - strand at 527.020 kb on + strand at 527.020 kb on + strand at 527.021 kb on - strand at 527.030 kb on + strand at 527.033 kb on + strand at 527.034 kb on - strand at 527.034 kb on - strand at 527.068 kb on - strand at 527.127 kb on - strand at 527.135 kb on + strand at 527.190 kb on + strand, within EX28DRAFT_0532 at 527.213 kb on + strand, within EX28DRAFT_0532 at 527.284 kb on + strand, within EX28DRAFT_0532 at 527.285 kb on - strand, within EX28DRAFT_0532 at 527.285 kb on - strand, within EX28DRAFT_0532 at 527.365 kb on - strand, within EX28DRAFT_0532 at 527.412 kb on - strand, within EX28DRAFT_0532 at 527.424 kb on + strand, within EX28DRAFT_0532 at 527.476 kb on - strand, within EX28DRAFT_0532 at 527.518 kb on + strand, within EX28DRAFT_0532 at 527.518 kb on + strand, within EX28DRAFT_0532 at 527.615 kb on + strand, within EX28DRAFT_0532 at 527.735 kb on + strand, within EX28DRAFT_0532 at 527.735 kb on + strand, within EX28DRAFT_0532 at 527.830 kb on + strand, within EX28DRAFT_0532 at 527.874 kb on - strand, within EX28DRAFT_0532 at 527.874 kb on - strand, within EX28DRAFT_0532 at 527.886 kb on + strand, within EX28DRAFT_0532 at 527.887 kb on - strand, within EX28DRAFT_0532 at 527.896 kb on + strand, within EX28DRAFT_0532 at 527.999 kb on + strand at 528.023 kb on + strand at 528.031 kb on + strand at 528.075 kb on + strand at 528.168 kb on + strand, within EX28DRAFT_0533 at 528.168 kb on + strand, within EX28DRAFT_0533 at 528.212 kb on + strand, within EX28DRAFT_0533 at 528.213 kb on - strand, within EX28DRAFT_0533 at 528.213 kb on - strand, within EX28DRAFT_0533 at 528.265 kb on + strand, within EX28DRAFT_0533 at 528.266 kb on - strand, within EX28DRAFT_0533 at 528.301 kb on - strand, within EX28DRAFT_0533 at 528.301 kb on - strand, within EX28DRAFT_0533 at 528.592 kb on + strand, within EX28DRAFT_0533 at 528.593 kb on - strand, within EX28DRAFT_0533 at 528.593 kb on - strand, within EX28DRAFT_0533 at 528.593 kb on - strand, within EX28DRAFT_0533 at 528.593 kb on - strand, within EX28DRAFT_0533 at 528.593 kb on - strand, within EX28DRAFT_0533 at 528.595 kb on - strand, within EX28DRAFT_0533 at 528.731 kb on + strand at 528.750 kb on + strand at 528.751 kb on - strand at 528.751 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media with m-arsenite 1 mM remove 526,103 + EX28DRAFT_0531 0.23 -0.2 526,176 + EX28DRAFT_0531 0.30 -1.7 526,177 - EX28DRAFT_0531 0.30 -2.5 526,195 - EX28DRAFT_0531 0.32 -2.2 526,220 + EX28DRAFT_0531 0.35 -3.3 526,221 - EX28DRAFT_0531 0.35 -0.9 526,248 - EX28DRAFT_0531 0.38 -3.3 526,251 + EX28DRAFT_0531 0.38 -0.6 526,253 + EX28DRAFT_0531 0.38 -2.2 526,290 + EX28DRAFT_0531 0.42 -4.5 526,291 - EX28DRAFT_0531 0.42 -1.1 526,296 + EX28DRAFT_0531 0.42 -2.0 526,331 + EX28DRAFT_0531 0.46 -2.2 526,371 - EX28DRAFT_0531 0.50 +1.2 526,371 - EX28DRAFT_0531 0.50 -1.8 526,383 - EX28DRAFT_0531 0.51 -1.8 526,489 + EX28DRAFT_0531 0.61 -1.4 526,793 - -4.0 526,929 + -0.2 526,930 - -0.8 526,935 - -0.0 526,975 + -0.5 526,976 - -0.4 526,976 - -0.1 527,013 + +0.8 527,013 + +0.8 527,014 - -0.9 527,014 - +0.9 527,014 - +0.1 527,014 - +0.5 527,014 - -0.4 527,015 + +0.3 527,015 + +0.4 527,015 + +0.1 527,015 + +0.9 527,015 + +0.0 527,015 + +0.0 527,015 + -1.7 527,016 - +0.1 527,016 - +0.4 527,016 - -0.1 527,020 + -0.3 527,020 + -0.0 527,021 - -0.5 527,030 + +0.1 527,033 + +0.6 527,034 - -1.0 527,034 - -0.7 527,068 - +1.2 527,127 - +0.7 527,135 + +0.2 527,190 + EX28DRAFT_0532 0.12 +0.4 527,213 + EX28DRAFT_0532 0.15 -3.1 527,284 + EX28DRAFT_0532 0.22 +0.8 527,285 - EX28DRAFT_0532 0.22 +0.2 527,285 - EX28DRAFT_0532 0.22 +1.1 527,365 - EX28DRAFT_0532 0.30 -0.6 527,412 - EX28DRAFT_0532 0.35 +0.5 527,424 + EX28DRAFT_0532 0.36 -0.3 527,476 - EX28DRAFT_0532 0.42 -0.3 527,518 + EX28DRAFT_0532 0.46 +0.6 527,518 + EX28DRAFT_0532 0.46 +0.3 527,615 + EX28DRAFT_0532 0.56 -0.7 527,735 + EX28DRAFT_0532 0.68 -1.1 527,735 + EX28DRAFT_0532 0.68 +0.3 527,830 + EX28DRAFT_0532 0.78 +0.3 527,874 - EX28DRAFT_0532 0.82 -0.3 527,874 - EX28DRAFT_0532 0.82 -1.0 527,886 + EX28DRAFT_0532 0.83 -0.1 527,887 - EX28DRAFT_0532 0.83 +1.0 527,896 + EX28DRAFT_0532 0.84 -0.0 527,999 + -1.2 528,023 + -0.2 528,031 + -0.3 528,075 + -0.5 528,168 + EX28DRAFT_0533 0.13 +0.5 528,168 + EX28DRAFT_0533 0.13 -1.2 528,212 + EX28DRAFT_0533 0.20 -3.5 528,213 - EX28DRAFT_0533 0.20 -3.0 528,213 - EX28DRAFT_0533 0.20 -2.2 528,265 + EX28DRAFT_0533 0.29 -1.3 528,266 - EX28DRAFT_0533 0.29 -4.0 528,301 - EX28DRAFT_0533 0.35 -1.6 528,301 - EX28DRAFT_0533 0.35 -4.9 528,592 + EX28DRAFT_0533 0.82 -4.3 528,593 - EX28DRAFT_0533 0.82 -0.5 528,593 - EX28DRAFT_0533 0.82 -1.5 528,593 - EX28DRAFT_0533 0.82 +0.1 528,593 - EX28DRAFT_0533 0.82 -1.5 528,593 - EX28DRAFT_0533 0.82 -2.4 528,595 - EX28DRAFT_0533 0.83 -3.5 528,731 + -3.8 528,750 + -4.0 528,751 - -4.9 528,751 - -3.6
Or see this region's nucleotide sequence