Experiment: MS_media with m-arsenite 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0511 and EX28DRAFT_0512 are separated by 12 nucleotides EX28DRAFT_0512 and EX28DRAFT_0513 are separated by 176 nucleotides
EX28DRAFT_0511: EX28DRAFT_0511 - colanic acid biosynthesis glycosyltransferase WcaL, at 504,433 to 505,653
_0511
EX28DRAFT_0512: EX28DRAFT_0512 - colanic acid biosynthesis protein WcaM, at 505,666 to 507,057
_0512
EX28DRAFT_0513: EX28DRAFT_0513 - UDP-glucose pyrophosphorylase regulatory subunit, at 507,234 to 508,130
_0513
Position (kb)
505
506
507
508 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 504.935 kb on - strand, within EX28DRAFT_0511 at 505.428 kb on - strand, within EX28DRAFT_0511 at 505.461 kb on + strand, within EX28DRAFT_0511 at 505.461 kb on + strand, within EX28DRAFT_0511 at 505.461 kb on + strand, within EX28DRAFT_0511 at 505.665 kb on + strand at 505.665 kb on + strand at 505.666 kb on - strand at 505.666 kb on - strand at 505.979 kb on + strand, within EX28DRAFT_0512 at 506.371 kb on + strand, within EX28DRAFT_0512 at 506.372 kb on - strand, within EX28DRAFT_0512 at 506.450 kb on + strand, within EX28DRAFT_0512 at 506.478 kb on + strand, within EX28DRAFT_0512 at 506.505 kb on + strand, within EX28DRAFT_0512 at 506.507 kb on + strand, within EX28DRAFT_0512 at 506.537 kb on + strand, within EX28DRAFT_0512 at 506.561 kb on - strand, within EX28DRAFT_0512 at 506.586 kb on + strand, within EX28DRAFT_0512 at 506.637 kb on - strand, within EX28DRAFT_0512 at 506.637 kb on - strand, within EX28DRAFT_0512 at 506.647 kb on + strand, within EX28DRAFT_0512 at 506.682 kb on + strand, within EX28DRAFT_0512 at 506.710 kb on + strand, within EX28DRAFT_0512 at 506.955 kb on + strand at 507.034 kb on - strand at 507.056 kb on - strand at 507.066 kb on + strand at 507.067 kb on - strand at 507.105 kb on + strand at 507.151 kb on + strand at 507.152 kb on - strand at 507.152 kb on - strand at 507.239 kb on + strand at 507.281 kb on + strand at 507.281 kb on + strand at 507.281 kb on + strand at 507.282 kb on - strand at 507.308 kb on + strand at 507.348 kb on + strand, within EX28DRAFT_0513 at 507.506 kb on + strand, within EX28DRAFT_0513 at 507.507 kb on - strand, within EX28DRAFT_0513 at 507.507 kb on - strand, within EX28DRAFT_0513 at 507.507 kb on - strand, within EX28DRAFT_0513 at 507.572 kb on + strand, within EX28DRAFT_0513 at 507.573 kb on - strand, within EX28DRAFT_0513 at 507.642 kb on - strand, within EX28DRAFT_0513 at 507.667 kb on - strand, within EX28DRAFT_0513 at 507.754 kb on - strand, within EX28DRAFT_0513 at 507.770 kb on + strand, within EX28DRAFT_0513 at 507.868 kb on + strand, within EX28DRAFT_0513 at 507.868 kb on + strand, within EX28DRAFT_0513 at 507.869 kb on - strand, within EX28DRAFT_0513 at 507.939 kb on - strand, within EX28DRAFT_0513 at 507.993 kb on - strand, within EX28DRAFT_0513 at 507.993 kb on - strand, within EX28DRAFT_0513 at 508.015 kb on - strand, within EX28DRAFT_0513 at 508.038 kb on + strand, within EX28DRAFT_0513 at 508.039 kb on - strand, within EX28DRAFT_0513 at 508.040 kb on + strand, within EX28DRAFT_0513 at 508.040 kb on + strand, within EX28DRAFT_0513 at 508.041 kb on - strand at 508.041 kb on - strand at 508.041 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media with m-arsenite 1 mM remove 504,935 - EX28DRAFT_0511 0.41 -0.3 505,428 - EX28DRAFT_0511 0.81 +0.9 505,461 + EX28DRAFT_0511 0.84 -0.4 505,461 + EX28DRAFT_0511 0.84 -0.1 505,461 + EX28DRAFT_0511 0.84 -0.2 505,665 + -0.6 505,665 + +0.4 505,666 - -1.9 505,666 - -0.4 505,979 + EX28DRAFT_0512 0.22 -0.6 506,371 + EX28DRAFT_0512 0.51 +0.4 506,372 - EX28DRAFT_0512 0.51 +0.1 506,450 + EX28DRAFT_0512 0.56 -1.1 506,478 + EX28DRAFT_0512 0.58 +0.7 506,505 + EX28DRAFT_0512 0.60 -0.2 506,507 + EX28DRAFT_0512 0.60 -0.0 506,537 + EX28DRAFT_0512 0.63 -0.1 506,561 - EX28DRAFT_0512 0.64 -0.3 506,586 + EX28DRAFT_0512 0.66 -0.8 506,637 - EX28DRAFT_0512 0.70 +0.6 506,637 - EX28DRAFT_0512 0.70 -0.5 506,647 + EX28DRAFT_0512 0.70 -1.4 506,682 + EX28DRAFT_0512 0.73 +0.1 506,710 + EX28DRAFT_0512 0.75 -0.3 506,955 + -0.1 507,034 - +0.3 507,056 - -0.1 507,066 + -0.2 507,067 - +0.7 507,105 + +0.6 507,151 + -0.7 507,152 - +0.3 507,152 - +0.3 507,239 + -0.7 507,281 + +0.8 507,281 + +0.2 507,281 + +0.6 507,282 - +1.0 507,308 + -1.3 507,348 + EX28DRAFT_0513 0.13 +1.0 507,506 + EX28DRAFT_0513 0.30 +0.1 507,507 - EX28DRAFT_0513 0.30 -1.0 507,507 - EX28DRAFT_0513 0.30 +0.1 507,507 - EX28DRAFT_0513 0.30 -1.3 507,572 + EX28DRAFT_0513 0.38 +0.0 507,573 - EX28DRAFT_0513 0.38 +0.4 507,642 - EX28DRAFT_0513 0.45 +0.2 507,667 - EX28DRAFT_0513 0.48 +0.6 507,754 - EX28DRAFT_0513 0.58 +0.9 507,770 + EX28DRAFT_0513 0.60 -0.2 507,868 + EX28DRAFT_0513 0.71 -0.6 507,868 + EX28DRAFT_0513 0.71 +0.6 507,869 - EX28DRAFT_0513 0.71 -0.5 507,939 - EX28DRAFT_0513 0.79 -0.5 507,993 - EX28DRAFT_0513 0.85 -0.5 507,993 - EX28DRAFT_0513 0.85 +2.1 508,015 - EX28DRAFT_0513 0.87 +0.2 508,038 + EX28DRAFT_0513 0.90 -0.2 508,039 - EX28DRAFT_0513 0.90 -0.2 508,040 + EX28DRAFT_0513 0.90 -0.3 508,040 + EX28DRAFT_0513 0.90 +0.1 508,041 - +1.8 508,041 - +0.1 508,041 - +0.9
Or see this region's nucleotide sequence