Experiment: MS_media with m-arsenite 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0500 and EX28DRAFT_0501 are separated by 15 nucleotides EX28DRAFT_0501 and EX28DRAFT_0502 are separated by 24 nucleotides EX28DRAFT_0502 and EX28DRAFT_0503 are separated by 2 nucleotides EX28DRAFT_0503 and EX28DRAFT_0504 are separated by 2 nucleotides
EX28DRAFT_0500: EX28DRAFT_0500 - colanic acid biosynthesis glycosyl transferase WcaE, at 492,131 to 492,877
_0500
EX28DRAFT_0501: EX28DRAFT_0501 - colanic acid biosynthesis acetyltransferase WcaF, at 492,893 to 493,441
_0501
EX28DRAFT_0502: EX28DRAFT_0502 - GDP-mannose 4,6-dehydratase, at 493,466 to 494,587
_0502
EX28DRAFT_0503: EX28DRAFT_0503 - Nucleoside-diphosphate-sugar epimerases, at 494,590 to 495,555
_0503
EX28DRAFT_0504: EX28DRAFT_0504 - ADP-ribose pyrophosphatase, at 495,558 to 496,037
_0504
Position (kb)
493
494
495 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 492.473 kb on + strand, within EX28DRAFT_0500 at 492.474 kb on - strand, within EX28DRAFT_0500 at 492.474 kb on - strand, within EX28DRAFT_0500 at 492.542 kb on + strand, within EX28DRAFT_0500 at 492.543 kb on - strand, within EX28DRAFT_0500 at 492.544 kb on + strand, within EX28DRAFT_0500 at 492.549 kb on - strand, within EX28DRAFT_0500 at 492.629 kb on - strand, within EX28DRAFT_0500 at 493.152 kb on - strand, within EX28DRAFT_0501 at 493.385 kb on - strand, within EX28DRAFT_0501 at 493.439 kb on + strand at 493.451 kb on + strand at 493.461 kb on + strand at 493.461 kb on + strand at 493.461 kb on + strand at 493.461 kb on + strand at 493.461 kb on + strand at 493.552 kb on - strand at 493.562 kb on - strand at 493.603 kb on + strand, within EX28DRAFT_0502 at 493.603 kb on + strand, within EX28DRAFT_0502 at 493.603 kb on + strand, within EX28DRAFT_0502 at 493.603 kb on + strand, within EX28DRAFT_0502 at 493.604 kb on - strand, within EX28DRAFT_0502 at 493.699 kb on - strand, within EX28DRAFT_0502 at 493.699 kb on - strand, within EX28DRAFT_0502 at 493.952 kb on + strand, within EX28DRAFT_0502 at 493.958 kb on + strand, within EX28DRAFT_0502 at 493.997 kb on + strand, within EX28DRAFT_0502 at 493.997 kb on + strand, within EX28DRAFT_0502 at 493.997 kb on + strand, within EX28DRAFT_0502 at 493.998 kb on - strand, within EX28DRAFT_0502 at 493.998 kb on - strand, within EX28DRAFT_0502 at 493.998 kb on - strand, within EX28DRAFT_0502 at 494.605 kb on - strand at 494.768 kb on - strand, within EX28DRAFT_0503 at 494.768 kb on - strand, within EX28DRAFT_0503 at 494.889 kb on + strand, within EX28DRAFT_0503 at 494.996 kb on - strand, within EX28DRAFT_0503 at 495.049 kb on + strand, within EX28DRAFT_0503 at 495.378 kb on - strand, within EX28DRAFT_0503 at 495.511 kb on - strand at 495.514 kb on + strand at 495.514 kb on + strand at 495.515 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media with m-arsenite 1 mM remove 492,473 + EX28DRAFT_0500 0.46 +0.1 492,474 - EX28DRAFT_0500 0.46 +0.0 492,474 - EX28DRAFT_0500 0.46 +0.4 492,542 + EX28DRAFT_0500 0.55 +0.6 492,543 - EX28DRAFT_0500 0.55 +1.2 492,544 + EX28DRAFT_0500 0.55 -0.6 492,549 - EX28DRAFT_0500 0.56 +0.2 492,629 - EX28DRAFT_0500 0.67 -0.5 493,152 - EX28DRAFT_0501 0.47 -0.2 493,385 - EX28DRAFT_0501 0.90 +0.1 493,439 + -0.4 493,451 + -0.6 493,461 + +0.3 493,461 + -0.6 493,461 + +0.2 493,461 + -0.9 493,461 + +0.8 493,552 - -0.5 493,562 - +0.1 493,603 + EX28DRAFT_0502 0.12 -0.9 493,603 + EX28DRAFT_0502 0.12 +0.5 493,603 + EX28DRAFT_0502 0.12 -0.2 493,603 + EX28DRAFT_0502 0.12 +1.6 493,604 - EX28DRAFT_0502 0.12 +0.1 493,699 - EX28DRAFT_0502 0.21 -0.5 493,699 - EX28DRAFT_0502 0.21 +0.1 493,952 + EX28DRAFT_0502 0.43 -0.3 493,958 + EX28DRAFT_0502 0.44 +0.2 493,997 + EX28DRAFT_0502 0.47 -2.0 493,997 + EX28DRAFT_0502 0.47 +2.9 493,997 + EX28DRAFT_0502 0.47 -0.4 493,998 - EX28DRAFT_0502 0.47 -0.9 493,998 - EX28DRAFT_0502 0.47 -0.1 493,998 - EX28DRAFT_0502 0.47 -0.6 494,605 - -1.4 494,768 - EX28DRAFT_0503 0.18 +0.0 494,768 - EX28DRAFT_0503 0.18 +0.1 494,889 + EX28DRAFT_0503 0.31 +0.8 494,996 - EX28DRAFT_0503 0.42 +0.1 495,049 + EX28DRAFT_0503 0.48 -0.0 495,378 - EX28DRAFT_0503 0.82 +1.0 495,511 - -1.1 495,514 + -0.2 495,514 + -0.5 495,515 - +0.2
Or see this region's nucleotide sequence