Experiment: MS_media with m-arsenite 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0447 and EX28DRAFT_0448 are separated by 18 nucleotides EX28DRAFT_0448 and EX28DRAFT_0449 are separated by 336 nucleotides
EX28DRAFT_0447: EX28DRAFT_0447 - Glutamate decarboxylase and related PLP-dependent proteins, at 428,400 to 429,866
_0447
EX28DRAFT_0448: EX28DRAFT_0448 - 2,4-diaminobutyrate 4-transaminases, at 429,885 to 431,258
_0448
EX28DRAFT_0449: EX28DRAFT_0449 - transcriptional regulator, LacI family, at 431,595 to 432,602
_0449
Position (kb)
429
430
431
432 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 428.890 kb on + strand, within EX28DRAFT_0447 at 428.891 kb on - strand, within EX28DRAFT_0447 at 429.537 kb on - strand, within EX28DRAFT_0447 at 429.751 kb on + strand at 429.751 kb on + strand at 429.752 kb on - strand at 429.815 kb on - strand at 429.815 kb on - strand at 429.869 kb on + strand at 429.870 kb on - strand at 429.877 kb on - strand at 429.912 kb on + strand at 430.305 kb on - strand, within EX28DRAFT_0448 at 430.389 kb on - strand, within EX28DRAFT_0448 at 430.596 kb on - strand, within EX28DRAFT_0448 at 430.637 kb on - strand, within EX28DRAFT_0448 at 430.639 kb on + strand, within EX28DRAFT_0448 at 430.639 kb on + strand, within EX28DRAFT_0448 at 430.639 kb on + strand, within EX28DRAFT_0448 at 430.691 kb on - strand, within EX28DRAFT_0448 at 430.731 kb on - strand, within EX28DRAFT_0448 at 430.907 kb on - strand, within EX28DRAFT_0448 at 430.907 kb on - strand, within EX28DRAFT_0448 at 430.943 kb on - strand, within EX28DRAFT_0448 at 430.970 kb on + strand, within EX28DRAFT_0448 at 430.985 kb on + strand, within EX28DRAFT_0448 at 430.986 kb on - strand, within EX28DRAFT_0448 at 431.007 kb on - strand, within EX28DRAFT_0448 at 431.077 kb on - strand, within EX28DRAFT_0448 at 431.089 kb on + strand, within EX28DRAFT_0448 at 431.090 kb on - strand, within EX28DRAFT_0448 at 431.090 kb on - strand, within EX28DRAFT_0448 at 431.096 kb on + strand, within EX28DRAFT_0448 at 431.132 kb on + strand at 431.161 kb on + strand at 431.204 kb on - strand at 431.229 kb on + strand at 431.230 kb on - strand at 431.230 kb on - strand at 431.230 kb on - strand at 431.230 kb on - strand at 431.230 kb on - strand at 431.271 kb on - strand at 431.351 kb on + strand at 431.380 kb on + strand at 431.454 kb on + strand at 431.565 kb on + strand at 431.597 kb on - strand at 431.675 kb on + strand at 431.676 kb on - strand at 431.676 kb on - strand at 431.676 kb on - strand at 431.679 kb on - strand at 431.765 kb on + strand, within EX28DRAFT_0449 at 431.832 kb on + strand, within EX28DRAFT_0449 at 431.832 kb on + strand, within EX28DRAFT_0449 at 431.832 kb on + strand, within EX28DRAFT_0449 at 431.833 kb on - strand, within EX28DRAFT_0449 at 431.922 kb on - strand, within EX28DRAFT_0449 at 431.929 kb on + strand, within EX28DRAFT_0449 at 431.951 kb on + strand, within EX28DRAFT_0449 at 431.951 kb on + strand, within EX28DRAFT_0449 at 431.952 kb on - strand, within EX28DRAFT_0449 at 431.953 kb on + strand, within EX28DRAFT_0449 at 431.953 kb on + strand, within EX28DRAFT_0449 at 431.953 kb on + strand, within EX28DRAFT_0449 at 431.953 kb on + strand, within EX28DRAFT_0449 at 431.953 kb on + strand, within EX28DRAFT_0449 at 431.953 kb on + strand, within EX28DRAFT_0449 at 431.953 kb on + strand, within EX28DRAFT_0449 at 431.954 kb on - strand, within EX28DRAFT_0449 at 431.954 kb on - strand, within EX28DRAFT_0449 at 432.105 kb on - strand, within EX28DRAFT_0449 at 432.107 kb on - strand, within EX28DRAFT_0449 at 432.133 kb on + strand, within EX28DRAFT_0449 at 432.219 kb on + strand, within EX28DRAFT_0449 at 432.219 kb on + strand, within EX28DRAFT_0449 at 432.220 kb on - strand, within EX28DRAFT_0449 at 432.235 kb on + strand, within EX28DRAFT_0449
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media with m-arsenite 1 mM remove 428,890 + EX28DRAFT_0447 0.33 -0.4 428,891 - EX28DRAFT_0447 0.33 -0.1 429,537 - EX28DRAFT_0447 0.78 -1.3 429,751 + -1.7 429,751 + -2.3 429,752 - -1.0 429,815 - -0.2 429,815 - -2.4 429,869 + -0.3 429,870 - -0.7 429,877 - -1.3 429,912 + +1.9 430,305 - EX28DRAFT_0448 0.31 -0.8 430,389 - EX28DRAFT_0448 0.37 -1.4 430,596 - EX28DRAFT_0448 0.52 -1.1 430,637 - EX28DRAFT_0448 0.55 -1.0 430,639 + EX28DRAFT_0448 0.55 +0.8 430,639 + EX28DRAFT_0448 0.55 -0.7 430,639 + EX28DRAFT_0448 0.55 -0.9 430,691 - EX28DRAFT_0448 0.59 -0.6 430,731 - EX28DRAFT_0448 0.62 +0.2 430,907 - EX28DRAFT_0448 0.74 -1.2 430,907 - EX28DRAFT_0448 0.74 +0.1 430,943 - EX28DRAFT_0448 0.77 -0.8 430,970 + EX28DRAFT_0448 0.79 -1.3 430,985 + EX28DRAFT_0448 0.80 -0.4 430,986 - EX28DRAFT_0448 0.80 -1.0 431,007 - EX28DRAFT_0448 0.82 -0.6 431,077 - EX28DRAFT_0448 0.87 -0.5 431,089 + EX28DRAFT_0448 0.88 -0.5 431,090 - EX28DRAFT_0448 0.88 -0.3 431,090 - EX28DRAFT_0448 0.88 -0.6 431,096 + EX28DRAFT_0448 0.88 +0.1 431,132 + -1.4 431,161 + -0.1 431,204 - -0.9 431,229 + -0.3 431,230 - -1.6 431,230 - -1.2 431,230 - -0.1 431,230 - +0.1 431,230 - -2.8 431,271 - -0.7 431,351 + -0.2 431,380 + -0.3 431,454 + -0.1 431,565 + +0.3 431,597 - +0.1 431,675 + -2.0 431,676 - +0.3 431,676 - +0.4 431,676 - +0.8 431,679 - +0.3 431,765 + EX28DRAFT_0449 0.17 +0.0 431,832 + EX28DRAFT_0449 0.24 -0.5 431,832 + EX28DRAFT_0449 0.24 -0.6 431,832 + EX28DRAFT_0449 0.24 +0.4 431,833 - EX28DRAFT_0449 0.24 -0.3 431,922 - EX28DRAFT_0449 0.32 -0.4 431,929 + EX28DRAFT_0449 0.33 +0.2 431,951 + EX28DRAFT_0449 0.35 +0.2 431,951 + EX28DRAFT_0449 0.35 +1.0 431,952 - EX28DRAFT_0449 0.35 +0.3 431,953 + EX28DRAFT_0449 0.36 +0.2 431,953 + EX28DRAFT_0449 0.36 +0.3 431,953 + EX28DRAFT_0449 0.36 +0.0 431,953 + EX28DRAFT_0449 0.36 +0.5 431,953 + EX28DRAFT_0449 0.36 +0.5 431,953 + EX28DRAFT_0449 0.36 +1.0 431,953 + EX28DRAFT_0449 0.36 +0.9 431,954 - EX28DRAFT_0449 0.36 +0.2 431,954 - EX28DRAFT_0449 0.36 -0.2 432,105 - EX28DRAFT_0449 0.51 -0.7 432,107 - EX28DRAFT_0449 0.51 +0.2 432,133 + EX28DRAFT_0449 0.53 -0.0 432,219 + EX28DRAFT_0449 0.62 +0.2 432,219 + EX28DRAFT_0449 0.62 -0.0 432,220 - EX28DRAFT_0449 0.62 +0.7 432,235 + EX28DRAFT_0449 0.63 +0.3
Or see this region's nucleotide sequence