Experiment: MS_media with m-arsenite 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0336 and EX28DRAFT_0337 are separated by 71 nucleotides EX28DRAFT_0337 and EX28DRAFT_0338 are separated by 53 nucleotides EX28DRAFT_0338 and EX28DRAFT_0339 are separated by 86 nucleotides EX28DRAFT_0339 and EX28DRAFT_0340 are separated by 88 nucleotides
EX28DRAFT_0336: EX28DRAFT_0336 - ribonuclease Z, at 298,720 to 299,637
_0336
EX28DRAFT_0337: EX28DRAFT_0337 - Predicted acyltransferase, at 299,709 to 300,170
_0337
EX28DRAFT_0338: EX28DRAFT_0338 - Uncharacterized conserved protein, at 300,224 to 300,529
_0338
EX28DRAFT_0339: EX28DRAFT_0339 - isochorismate synthase (EC 5.4.4.2), at 300,616 to 301,911
_0339
EX28DRAFT_0340: EX28DRAFT_0340 - 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylic-acid synthase, at 302,000 to 303,670
_0340
Position (kb)
300
301
302 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 299.617 kb on - strand at 299.673 kb on + strand at 299.674 kb on - strand at 299.676 kb on + strand at 299.678 kb on + strand at 299.729 kb on - strand at 299.729 kb on - strand at 299.729 kb on - strand at 299.729 kb on - strand at 299.814 kb on + strand, within EX28DRAFT_0337 at 299.859 kb on - strand, within EX28DRAFT_0337 at 299.893 kb on - strand, within EX28DRAFT_0337 at 300.045 kb on + strand, within EX28DRAFT_0337 at 300.046 kb on - strand, within EX28DRAFT_0337 at 300.078 kb on + strand, within EX28DRAFT_0337 at 300.079 kb on - strand, within EX28DRAFT_0337 at 300.143 kb on + strand at 300.188 kb on + strand at 300.189 kb on - strand at 300.206 kb on - strand at 300.208 kb on + strand at 300.208 kb on + strand at 300.209 kb on - strand at 300.209 kb on - strand at 300.224 kb on - strand at 300.297 kb on + strand, within EX28DRAFT_0338 at 300.297 kb on + strand, within EX28DRAFT_0338 at 300.329 kb on + strand, within EX28DRAFT_0338 at 300.329 kb on + strand, within EX28DRAFT_0338 at 300.330 kb on - strand, within EX28DRAFT_0338 at 300.331 kb on + strand, within EX28DRAFT_0338 at 300.331 kb on + strand, within EX28DRAFT_0338 at 300.331 kb on + strand, within EX28DRAFT_0338 at 300.332 kb on - strand, within EX28DRAFT_0338 at 300.400 kb on + strand, within EX28DRAFT_0338 at 300.400 kb on + strand, within EX28DRAFT_0338 at 300.400 kb on + strand, within EX28DRAFT_0338 at 300.401 kb on - strand, within EX28DRAFT_0338 at 300.404 kb on + strand, within EX28DRAFT_0338 at 300.405 kb on - strand, within EX28DRAFT_0338 at 300.405 kb on - strand, within EX28DRAFT_0338 at 300.405 kb on - strand, within EX28DRAFT_0338 at 300.407 kb on + strand, within EX28DRAFT_0338 at 300.431 kb on + strand, within EX28DRAFT_0338 at 300.431 kb on + strand, within EX28DRAFT_0338 at 300.446 kb on + strand, within EX28DRAFT_0338 at 300.446 kb on + strand, within EX28DRAFT_0338 at 300.447 kb on - strand, within EX28DRAFT_0338 at 300.548 kb on - strand at 300.548 kb on - strand at 300.549 kb on + strand at 300.549 kb on + strand at 300.550 kb on - strand at 300.550 kb on - strand at 300.563 kb on + strand at 300.563 kb on + strand at 300.564 kb on - strand at 300.568 kb on - strand at 300.568 kb on - strand at 300.586 kb on - strand at 300.589 kb on - strand at 300.589 kb on - strand at 300.689 kb on + strand at 300.775 kb on + strand, within EX28DRAFT_0339 at 301.071 kb on + strand, within EX28DRAFT_0339 at 301.072 kb on - strand, within EX28DRAFT_0339 at 301.271 kb on + strand, within EX28DRAFT_0339 at 301.279 kb on + strand, within EX28DRAFT_0339 at 301.470 kb on + strand, within EX28DRAFT_0339 at 301.470 kb on + strand, within EX28DRAFT_0339 at 301.488 kb on + strand, within EX28DRAFT_0339 at 301.529 kb on + strand, within EX28DRAFT_0339 at 301.558 kb on - strand, within EX28DRAFT_0339 at 301.632 kb on - strand, within EX28DRAFT_0339 at 301.739 kb on + strand, within EX28DRAFT_0339 at 301.824 kb on + strand at 301.872 kb on + strand at 301.910 kb on - strand at 301.937 kb on + strand at 301.965 kb on + strand at 301.965 kb on + strand at 301.966 kb on - strand at 301.966 kb on - strand at 301.966 kb on - strand at 301.968 kb on - strand at 302.018 kb on - strand at 302.081 kb on - strand at 302.081 kb on - strand at 302.081 kb on - strand at 302.304 kb on + strand, within EX28DRAFT_0340 at 302.425 kb on + strand, within EX28DRAFT_0340 at 302.426 kb on - strand, within EX28DRAFT_0340 at 302.426 kb on - strand, within EX28DRAFT_0340 at 302.495 kb on - strand, within EX28DRAFT_0340 at 302.522 kb on + strand, within EX28DRAFT_0340
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media with m-arsenite 1 mM remove 299,617 - -0.4 299,673 + -0.4 299,674 - -1.0 299,676 + +1.6 299,678 + +0.2 299,729 - -0.3 299,729 - -0.1 299,729 - -0.4 299,729 - +1.5 299,814 + EX28DRAFT_0337 0.23 -1.3 299,859 - EX28DRAFT_0337 0.32 -0.2 299,893 - EX28DRAFT_0337 0.40 -0.5 300,045 + EX28DRAFT_0337 0.73 +0.2 300,046 - EX28DRAFT_0337 0.73 -0.7 300,078 + EX28DRAFT_0337 0.80 -0.8 300,079 - EX28DRAFT_0337 0.80 +0.6 300,143 + +0.2 300,188 + -0.5 300,189 - -0.0 300,206 - +0.2 300,208 + +0.0 300,208 + -0.0 300,209 - -0.2 300,209 - -1.2 300,224 - +0.5 300,297 + EX28DRAFT_0338 0.24 +0.6 300,297 + EX28DRAFT_0338 0.24 -0.6 300,329 + EX28DRAFT_0338 0.34 +0.4 300,329 + EX28DRAFT_0338 0.34 -1.4 300,330 - EX28DRAFT_0338 0.35 -0.5 300,331 + EX28DRAFT_0338 0.35 -0.2 300,331 + EX28DRAFT_0338 0.35 +0.3 300,331 + EX28DRAFT_0338 0.35 +1.5 300,332 - EX28DRAFT_0338 0.35 -0.2 300,400 + EX28DRAFT_0338 0.58 +0.2 300,400 + EX28DRAFT_0338 0.58 +0.3 300,400 + EX28DRAFT_0338 0.58 +0.0 300,401 - EX28DRAFT_0338 0.58 +0.0 300,404 + EX28DRAFT_0338 0.59 -0.1 300,405 - EX28DRAFT_0338 0.59 +0.1 300,405 - EX28DRAFT_0338 0.59 -0.8 300,405 - EX28DRAFT_0338 0.59 +0.0 300,407 + EX28DRAFT_0338 0.60 +0.7 300,431 + EX28DRAFT_0338 0.68 -0.1 300,431 + EX28DRAFT_0338 0.68 +0.6 300,446 + EX28DRAFT_0338 0.73 -1.0 300,446 + EX28DRAFT_0338 0.73 +0.1 300,447 - EX28DRAFT_0338 0.73 +0.4 300,548 - -0.4 300,548 - -0.2 300,549 + -0.4 300,549 + +2.1 300,550 - -0.1 300,550 - -0.2 300,563 + -0.6 300,563 + +0.1 300,564 - -0.1 300,568 - +2.0 300,568 - -0.2 300,586 - +0.9 300,589 - -2.2 300,589 - +0.4 300,689 + -1.2 300,775 + EX28DRAFT_0339 0.12 +0.2 301,071 + EX28DRAFT_0339 0.35 -0.7 301,072 - EX28DRAFT_0339 0.35 -0.3 301,271 + EX28DRAFT_0339 0.51 +0.0 301,279 + EX28DRAFT_0339 0.51 -0.0 301,470 + EX28DRAFT_0339 0.66 +0.8 301,470 + EX28DRAFT_0339 0.66 -0.1 301,488 + EX28DRAFT_0339 0.67 +1.1 301,529 + EX28DRAFT_0339 0.70 +0.3 301,558 - EX28DRAFT_0339 0.73 +0.2 301,632 - EX28DRAFT_0339 0.78 +0.7 301,739 + EX28DRAFT_0339 0.87 -0.3 301,824 + -0.1 301,872 + +0.7 301,910 - -1.0 301,937 + -2.2 301,965 + -0.3 301,965 + -0.2 301,966 - +0.1 301,966 - +0.2 301,966 - -0.6 301,968 - +0.3 302,018 - -0.2 302,081 - -0.8 302,081 - -0.1 302,081 - -0.1 302,304 + EX28DRAFT_0340 0.18 +0.1 302,425 + EX28DRAFT_0340 0.25 +1.8 302,426 - EX28DRAFT_0340 0.25 +0.2 302,426 - EX28DRAFT_0340 0.25 -0.7 302,495 - EX28DRAFT_0340 0.30 +0.7 302,522 + EX28DRAFT_0340 0.31 +1.3
Or see this region's nucleotide sequence