Experiment: MS_media with m-arsenite 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0209 and EX28DRAFT_0210 are separated by 5 nucleotides EX28DRAFT_0210 and EX28DRAFT_0211 are separated by 44 nucleotides EX28DRAFT_0211 and EX28DRAFT_0212 are separated by 40 nucleotides
EX28DRAFT_0209: EX28DRAFT_0209 - cysteine synthase B, at 163,470 to 164,381
_0209
EX28DRAFT_0210: EX28DRAFT_0210 - pyridoxal kinase, at 164,387 to 165,223
_0210
EX28DRAFT_0211: EX28DRAFT_0211 - PTS system, glucose subfamily, IIA component, at 165,268 to 165,777
_0211
EX28DRAFT_0212: EX28DRAFT_0212 - phosphoenolpyruvate-protein phosphotransferase, at 165,818 to 167,545
_0212
Position (kb)
164
165
166 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 163.406 kb on + strand at 163.455 kb on + strand at 164.359 kb on - strand at 164.379 kb on + strand at 164.379 kb on + strand at 164.457 kb on + strand at 164.457 kb on + strand at 164.498 kb on - strand, within EX28DRAFT_0210 at 164.545 kb on + strand, within EX28DRAFT_0210 at 164.546 kb on - strand, within EX28DRAFT_0210 at 164.614 kb on + strand, within EX28DRAFT_0210 at 164.615 kb on - strand, within EX28DRAFT_0210 at 164.672 kb on - strand, within EX28DRAFT_0210 at 164.785 kb on + strand, within EX28DRAFT_0210 at 164.786 kb on - strand, within EX28DRAFT_0210 at 164.789 kb on + strand, within EX28DRAFT_0210 at 164.790 kb on - strand, within EX28DRAFT_0210 at 164.790 kb on - strand, within EX28DRAFT_0210 at 164.852 kb on - strand, within EX28DRAFT_0210 at 164.864 kb on + strand, within EX28DRAFT_0210 at 164.880 kb on + strand, within EX28DRAFT_0210 at 164.899 kb on + strand, within EX28DRAFT_0210 at 165.180 kb on - strand at 165.180 kb on - strand at 165.197 kb on + strand at 165.208 kb on + strand at 165.209 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media with m-arsenite 1 mM remove 163,406 + +0.4 163,455 + -0.4 164,359 - +0.3 164,379 + -0.7 164,379 + -1.4 164,457 + +0.1 164,457 + -0.0 164,498 - EX28DRAFT_0210 0.13 +0.0 164,545 + EX28DRAFT_0210 0.19 -0.2 164,546 - EX28DRAFT_0210 0.19 +0.8 164,614 + EX28DRAFT_0210 0.27 -2.7 164,615 - EX28DRAFT_0210 0.27 -0.4 164,672 - EX28DRAFT_0210 0.34 +0.1 164,785 + EX28DRAFT_0210 0.48 -0.1 164,786 - EX28DRAFT_0210 0.48 -0.0 164,789 + EX28DRAFT_0210 0.48 -2.2 164,790 - EX28DRAFT_0210 0.48 -0.1 164,790 - EX28DRAFT_0210 0.48 +1.6 164,852 - EX28DRAFT_0210 0.56 +0.9 164,864 + EX28DRAFT_0210 0.57 +2.6 164,880 + EX28DRAFT_0210 0.59 -0.7 164,899 + EX28DRAFT_0210 0.61 +0.4 165,180 - +0.0 165,180 - +0.4 165,197 + +0.3 165,208 + -0.2 165,209 - +0.0
Or see this region's nucleotide sequence