Experiment: MS_media with m-arsenite 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0193 and EX28DRAFT_0194 are separated by 66 nucleotides EX28DRAFT_0194 and EX28DRAFT_0195 are separated by 101 nucleotides
EX28DRAFT_0193: EX28DRAFT_0193 - glutamate synthase small subunit family protein, proteobacterial, at 145,432 to 147,408
_0193
EX28DRAFT_0194: EX28DRAFT_0194 - nudix-type nucleoside diphosphatase, YffH/AdpP family, at 147,475 to 148,068
_0194
EX28DRAFT_0195: EX28DRAFT_0195 - Protein of unknown function (DUF1176), at 148,170 to 149,213
_0195
Position (kb)
147
148
149 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 146.495 kb on - strand, within EX28DRAFT_0193 at 146.514 kb on - strand, within EX28DRAFT_0193 at 146.585 kb on + strand, within EX28DRAFT_0193 at 146.585 kb on + strand, within EX28DRAFT_0193 at 146.586 kb on - strand, within EX28DRAFT_0193 at 146.589 kb on + strand, within EX28DRAFT_0193 at 146.654 kb on + strand, within EX28DRAFT_0193 at 146.666 kb on - strand, within EX28DRAFT_0193 at 146.666 kb on - strand, within EX28DRAFT_0193 at 146.679 kb on + strand, within EX28DRAFT_0193 at 146.695 kb on + strand, within EX28DRAFT_0193 at 146.696 kb on - strand, within EX28DRAFT_0193 at 146.834 kb on + strand, within EX28DRAFT_0193 at 146.835 kb on - strand, within EX28DRAFT_0193 at 147.406 kb on + strand at 147.441 kb on - strand at 147.441 kb on - strand at 147.443 kb on + strand at 147.444 kb on - strand at 147.444 kb on - strand at 147.445 kb on + strand at 147.446 kb on - strand at 147.523 kb on + strand at 147.537 kb on + strand, within EX28DRAFT_0194 at 147.547 kb on + strand, within EX28DRAFT_0194 at 147.547 kb on + strand, within EX28DRAFT_0194 at 147.555 kb on + strand, within EX28DRAFT_0194 at 147.598 kb on + strand, within EX28DRAFT_0194 at 147.599 kb on - strand, within EX28DRAFT_0194 at 147.631 kb on + strand, within EX28DRAFT_0194 at 147.631 kb on + strand, within EX28DRAFT_0194 at 147.631 kb on + strand, within EX28DRAFT_0194 at 147.632 kb on - strand, within EX28DRAFT_0194 at 147.632 kb on - strand, within EX28DRAFT_0194 at 147.827 kb on + strand, within EX28DRAFT_0194 at 147.828 kb on - strand, within EX28DRAFT_0194 at 147.828 kb on - strand, within EX28DRAFT_0194 at 147.831 kb on + strand, within EX28DRAFT_0194 at 147.831 kb on + strand, within EX28DRAFT_0194 at 147.831 kb on + strand, within EX28DRAFT_0194 at 147.832 kb on - strand, within EX28DRAFT_0194 at 147.832 kb on - strand, within EX28DRAFT_0194 at 147.881 kb on - strand, within EX28DRAFT_0194 at 147.999 kb on + strand, within EX28DRAFT_0194 at 148.067 kb on - strand at 148.084 kb on + strand at 148.092 kb on - strand at 148.092 kb on - strand at 148.128 kb on + strand at 148.268 kb on - strand at 148.500 kb on - strand, within EX28DRAFT_0195 at 148.500 kb on - strand, within EX28DRAFT_0195 at 148.577 kb on + strand, within EX28DRAFT_0195 at 148.786 kb on - strand, within EX28DRAFT_0195 at 148.906 kb on - strand, within EX28DRAFT_0195 at 148.908 kb on - strand, within EX28DRAFT_0195
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media with m-arsenite 1 mM remove 146,495 - EX28DRAFT_0193 0.54 +0.2 146,514 - EX28DRAFT_0193 0.55 +0.5 146,585 + EX28DRAFT_0193 0.58 -0.5 146,585 + EX28DRAFT_0193 0.58 +0.5 146,586 - EX28DRAFT_0193 0.58 +0.5 146,589 + EX28DRAFT_0193 0.59 +0.5 146,654 + EX28DRAFT_0193 0.62 -0.3 146,666 - EX28DRAFT_0193 0.62 +0.3 146,666 - EX28DRAFT_0193 0.62 -0.2 146,679 + EX28DRAFT_0193 0.63 +0.6 146,695 + EX28DRAFT_0193 0.64 +1.3 146,696 - EX28DRAFT_0193 0.64 -1.3 146,834 + EX28DRAFT_0193 0.71 -1.0 146,835 - EX28DRAFT_0193 0.71 -0.7 147,406 + -0.6 147,441 - -0.6 147,441 - +1.0 147,443 + +0.2 147,444 - -0.0 147,444 - -2.9 147,445 + +0.2 147,446 - -0.4 147,523 + +2.6 147,537 + EX28DRAFT_0194 0.10 +0.4 147,547 + EX28DRAFT_0194 0.12 +0.3 147,547 + EX28DRAFT_0194 0.12 -1.0 147,555 + EX28DRAFT_0194 0.13 +0.2 147,598 + EX28DRAFT_0194 0.21 +0.3 147,599 - EX28DRAFT_0194 0.21 +0.1 147,631 + EX28DRAFT_0194 0.26 -1.0 147,631 + EX28DRAFT_0194 0.26 +0.1 147,631 + EX28DRAFT_0194 0.26 -0.1 147,632 - EX28DRAFT_0194 0.26 -0.1 147,632 - EX28DRAFT_0194 0.26 -0.3 147,827 + EX28DRAFT_0194 0.59 -0.0 147,828 - EX28DRAFT_0194 0.59 +0.1 147,828 - EX28DRAFT_0194 0.59 +0.3 147,831 + EX28DRAFT_0194 0.60 -0.7 147,831 + EX28DRAFT_0194 0.60 -0.1 147,831 + EX28DRAFT_0194 0.60 +2.2 147,832 - EX28DRAFT_0194 0.60 +1.2 147,832 - EX28DRAFT_0194 0.60 +0.6 147,881 - EX28DRAFT_0194 0.68 +0.3 147,999 + EX28DRAFT_0194 0.88 +0.8 148,067 - +0.2 148,084 + -0.2 148,092 - -0.4 148,092 - +0.1 148,128 + +0.4 148,268 - -0.2 148,500 - EX28DRAFT_0195 0.32 -0.4 148,500 - EX28DRAFT_0195 0.32 +0.1 148,577 + EX28DRAFT_0195 0.39 -0.3 148,786 - EX28DRAFT_0195 0.59 +0.4 148,906 - EX28DRAFT_0195 0.70 +0.8 148,908 - EX28DRAFT_0195 0.71 +0.2
Or see this region's nucleotide sequence