Experiment: MS_media with m-arsenite 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0184 and EX28DRAFT_0185 are separated by 120 nucleotides EX28DRAFT_0185 and EX28DRAFT_0186 overlap by 1 nucleotides EX28DRAFT_0186 and EX28DRAFT_0187 are separated by 73 nucleotides EX28DRAFT_0187 and EX28DRAFT_0188 are separated by 38 nucleotides
EX28DRAFT_0184: EX28DRAFT_0184 - phosphoribosylaminoimidazole-succinocarboxamide synthase (EC 6.3.2.6), at 133,605 to 134,318
_0184
EX28DRAFT_0185: EX28DRAFT_0185 - Predicted metalloprotease, at 134,439 to 135,308
_0185
EX28DRAFT_0186: EX28DRAFT_0186 - Predicted P-loop ATPase fused to an acetyltransferase, at 135,308 to 137,254
_0186
EX28DRAFT_0187: EX28DRAFT_0187 - Predicted esterase, at 137,328 to 138,023
_0187
EX28DRAFT_0188: EX28DRAFT_0188 - Uncharacterised protein family (UPF0370), at 138,062 to 138,259
_0188
Position (kb) 135
136
137
138 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 134.361 kb on - strand at 134.378 kb on + strand at 134.407 kb on - strand at 134.407 kb on - strand at 134.410 kb on + strand at 134.410 kb on + strand at 134.411 kb on - strand at 134.411 kb on - strand at 134.415 kb on + strand at 134.415 kb on + strand at 134.415 kb on + strand at 134.415 kb on + strand at 134.415 kb on + strand at 134.473 kb on + strand at 134.473 kb on + strand at 134.586 kb on + strand, within EX28DRAFT_0185 at 134.781 kb on + strand, within EX28DRAFT_0185 at 134.839 kb on - strand, within EX28DRAFT_0185 at 134.931 kb on + strand, within EX28DRAFT_0185 at 134.931 kb on + strand, within EX28DRAFT_0185 at 134.932 kb on - strand, within EX28DRAFT_0185 at 135.140 kb on - strand, within EX28DRAFT_0185 at 135.239 kb on + strand at 135.240 kb on - strand at 135.240 kb on - strand at 135.284 kb on + strand at 135.285 kb on - strand at 135.285 kb on - strand at 135.287 kb on + strand at 135.288 kb on - strand at 135.288 kb on - strand at 135.306 kb on + strand at 135.307 kb on - strand at 135.307 kb on - strand at 135.307 kb on - strand at 135.764 kb on - strand, within EX28DRAFT_0186 at 136.148 kb on + strand, within EX28DRAFT_0186 at 136.148 kb on + strand, within EX28DRAFT_0186 at 136.149 kb on - strand, within EX28DRAFT_0186 at 136.177 kb on + strand, within EX28DRAFT_0186 at 136.177 kb on + strand, within EX28DRAFT_0186 at 136.178 kb on - strand, within EX28DRAFT_0186 at 136.218 kb on + strand, within EX28DRAFT_0186 at 136.219 kb on - strand, within EX28DRAFT_0186 at 136.219 kb on - strand, within EX28DRAFT_0186 at 136.219 kb on - strand, within EX28DRAFT_0186 at 136.223 kb on - strand, within EX28DRAFT_0186 at 136.384 kb on - strand, within EX28DRAFT_0186 at 136.384 kb on - strand, within EX28DRAFT_0186 at 136.385 kb on + strand, within EX28DRAFT_0186 at 136.665 kb on + strand, within EX28DRAFT_0186 at 136.665 kb on + strand, within EX28DRAFT_0186 at 136.665 kb on + strand, within EX28DRAFT_0186 at 136.753 kb on + strand, within EX28DRAFT_0186 at 136.839 kb on - strand, within EX28DRAFT_0186 at 137.264 kb on + strand at 137.312 kb on + strand at 137.312 kb on + strand at 137.312 kb on + strand at 137.313 kb on - strand at 137.380 kb on + strand at 137.402 kb on - strand, within EX28DRAFT_0187 at 137.421 kb on - strand, within EX28DRAFT_0187 at 137.913 kb on - strand, within EX28DRAFT_0187 at 137.962 kb on - strand at 137.962 kb on - strand at 138.017 kb on + strand at 138.063 kb on + strand at 138.241 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media with m-arsenite 1 mM remove 134,361 - -0.5 134,378 + +0.2 134,407 - +0.3 134,407 - +0.0 134,410 + +0.8 134,410 + +0.7 134,411 - +0.7 134,411 - +0.1 134,415 + +1.1 134,415 + -0.2 134,415 + -0.1 134,415 + -0.3 134,415 + -0.0 134,473 + -1.0 134,473 + -0.1 134,586 + EX28DRAFT_0185 0.17 -0.1 134,781 + EX28DRAFT_0185 0.39 +0.0 134,839 - EX28DRAFT_0185 0.46 +0.1 134,931 + EX28DRAFT_0185 0.57 +0.3 134,931 + EX28DRAFT_0185 0.57 +2.7 134,932 - EX28DRAFT_0185 0.57 -0.1 135,140 - EX28DRAFT_0185 0.81 +0.5 135,239 + -0.4 135,240 - -3.4 135,240 - -0.5 135,284 + -0.2 135,285 - +0.4 135,285 - +1.3 135,287 + +0.1 135,288 - +0.5 135,288 - +0.1 135,306 + -0.1 135,307 - +0.0 135,307 - +1.2 135,307 - -0.3 135,764 - EX28DRAFT_0186 0.23 +0.8 136,148 + EX28DRAFT_0186 0.43 +1.4 136,148 + EX28DRAFT_0186 0.43 +0.5 136,149 - EX28DRAFT_0186 0.43 -0.0 136,177 + EX28DRAFT_0186 0.45 +1.3 136,177 + EX28DRAFT_0186 0.45 +0.0 136,178 - EX28DRAFT_0186 0.45 +2.2 136,218 + EX28DRAFT_0186 0.47 +2.1 136,219 - EX28DRAFT_0186 0.47 +0.7 136,219 - EX28DRAFT_0186 0.47 +0.8 136,219 - EX28DRAFT_0186 0.47 -0.4 136,223 - EX28DRAFT_0186 0.47 -0.3 136,384 - EX28DRAFT_0186 0.55 +0.1 136,384 - EX28DRAFT_0186 0.55 +1.0 136,385 + EX28DRAFT_0186 0.55 +1.7 136,665 + EX28DRAFT_0186 0.70 -0.6 136,665 + EX28DRAFT_0186 0.70 +1.8 136,665 + EX28DRAFT_0186 0.70 +0.6 136,753 + EX28DRAFT_0186 0.74 +0.2 136,839 - EX28DRAFT_0186 0.79 +1.0 137,264 + +0.5 137,312 + -0.7 137,312 + +0.1 137,312 + -0.2 137,313 - -0.7 137,380 + -1.3 137,402 - EX28DRAFT_0187 0.11 +0.8 137,421 - EX28DRAFT_0187 0.13 -0.4 137,913 - EX28DRAFT_0187 0.84 -0.2 137,962 - -0.9 137,962 - -0.0 138,017 + +0.4 138,063 + -0.9 138,241 - +0.2
Or see this region's nucleotide sequence