Experiment: MS_media with m-arsenite 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0040 and EX28DRAFT_0041 are separated by 25 nucleotides EX28DRAFT_0041 and EX28DRAFT_0042 are separated by 112 nucleotides EX28DRAFT_0042 and EX28DRAFT_0043 overlap by 29 nucleotides EX28DRAFT_0043 and EX28DRAFT_0044 are separated by 26 nucleotides
EX28DRAFT_0040: EX28DRAFT_0040 - methionyl-tRNA formyltransferase, at 20,391 to 21,338
_0040
EX28DRAFT_0041: EX28DRAFT_0041 - peptide deformylase, at 21,364 to 21,873
_0041
EX28DRAFT_0042: EX28DRAFT_0042 - DNA protecting protein DprA, at 21,986 to 23,125
_0042
EX28DRAFT_0043: EX28DRAFT_0043 - Uncharacterized protein conserved in bacteria, at 23,097 to 23,570
_0043
EX28DRAFT_0044: EX28DRAFT_0044 - Zn-finger domain associated with topoisomerase type I, at 23,597 to 24,151
_0044
Position (kb)
21
22
23
24 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 21.398 kb on - strand at 21.904 kb on - strand at 21.910 kb on - strand at 21.919 kb on - strand at 21.924 kb on - strand at 22.032 kb on + strand at 22.032 kb on + strand at 22.033 kb on - strand at 22.033 kb on - strand at 22.059 kb on + strand at 22.060 kb on - strand at 22.060 kb on - strand at 22.060 kb on - strand at 22.288 kb on - strand, within EX28DRAFT_0042 at 22.291 kb on + strand, within EX28DRAFT_0042 at 22.315 kb on + strand, within EX28DRAFT_0042 at 22.315 kb on + strand, within EX28DRAFT_0042 at 22.318 kb on + strand, within EX28DRAFT_0042 at 22.318 kb on + strand, within EX28DRAFT_0042 at 22.665 kb on + strand, within EX28DRAFT_0042 at 22.761 kb on + strand, within EX28DRAFT_0042 at 22.765 kb on + strand, within EX28DRAFT_0042 at 22.766 kb on - strand, within EX28DRAFT_0042 at 22.827 kb on + strand, within EX28DRAFT_0042 at 22.853 kb on + strand, within EX28DRAFT_0042 at 22.853 kb on + strand, within EX28DRAFT_0042 at 22.950 kb on + strand, within EX28DRAFT_0042 at 22.951 kb on - strand, within EX28DRAFT_0042 at 22.962 kb on + strand, within EX28DRAFT_0042 at 22.962 kb on + strand, within EX28DRAFT_0042 at 23.041 kb on - strand at 23.107 kb on + strand at 23.108 kb on - strand at 23.108 kb on - strand at 23.115 kb on + strand at 23.115 kb on + strand at 23.116 kb on - strand at 23.130 kb on + strand at 23.130 kb on + strand at 23.130 kb on + strand at 23.131 kb on - strand at 23.217 kb on + strand, within EX28DRAFT_0043 at 23.218 kb on - strand, within EX28DRAFT_0043 at 23.314 kb on - strand, within EX28DRAFT_0043 at 23.558 kb on + strand at 23.558 kb on + strand at 23.559 kb on - strand at 23.559 kb on - strand at 23.569 kb on - strand at 23.569 kb on - strand at 23.596 kb on + strand at 23.614 kb on - strand at 23.723 kb on + strand, within EX28DRAFT_0044 at 23.723 kb on + strand, within EX28DRAFT_0044 at 23.724 kb on - strand, within EX28DRAFT_0044 at 23.724 kb on - strand, within EX28DRAFT_0044 at 23.758 kb on + strand, within EX28DRAFT_0044 at 23.759 kb on - strand, within EX28DRAFT_0044 at 23.808 kb on + strand, within EX28DRAFT_0044 at 23.872 kb on + strand, within EX28DRAFT_0044 at 23.872 kb on + strand, within EX28DRAFT_0044 at 23.873 kb on - strand, within EX28DRAFT_0044 at 23.873 kb on - strand, within EX28DRAFT_0044 at 23.940 kb on + strand, within EX28DRAFT_0044 at 23.963 kb on - strand, within EX28DRAFT_0044 at 23.963 kb on - strand, within EX28DRAFT_0044 at 24.058 kb on - strand, within EX28DRAFT_0044 at 24.063 kb on + strand, within EX28DRAFT_0044 at 24.107 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media with m-arsenite 1 mM remove 21,398 - -1.6 21,904 - +0.3 21,910 - +0.6 21,919 - +0.1 21,924 - +1.3 22,032 + -0.7 22,032 + -0.2 22,033 - +0.2 22,033 - -0.4 22,059 + +0.8 22,060 - -0.1 22,060 - +0.6 22,060 - +0.4 22,288 - EX28DRAFT_0042 0.26 +0.3 22,291 + EX28DRAFT_0042 0.27 +0.1 22,315 + EX28DRAFT_0042 0.29 +1.1 22,315 + EX28DRAFT_0042 0.29 -0.0 22,318 + EX28DRAFT_0042 0.29 +0.2 22,318 + EX28DRAFT_0042 0.29 +0.5 22,665 + EX28DRAFT_0042 0.60 +0.5 22,761 + EX28DRAFT_0042 0.68 -0.4 22,765 + EX28DRAFT_0042 0.68 -0.9 22,766 - EX28DRAFT_0042 0.68 +0.5 22,827 + EX28DRAFT_0042 0.74 +0.1 22,853 + EX28DRAFT_0042 0.76 +1.4 22,853 + EX28DRAFT_0042 0.76 -2.3 22,950 + EX28DRAFT_0042 0.85 +1.1 22,951 - EX28DRAFT_0042 0.85 +0.1 22,962 + EX28DRAFT_0042 0.86 +0.4 22,962 + EX28DRAFT_0042 0.86 +1.2 23,041 - +0.5 23,107 + -1.3 23,108 - +0.7 23,108 - +0.1 23,115 + -0.1 23,115 + +0.7 23,116 - +0.2 23,130 + +0.3 23,130 + -2.2 23,130 + +0.2 23,131 - +0.5 23,217 + EX28DRAFT_0043 0.25 -2.6 23,218 - EX28DRAFT_0043 0.26 -0.1 23,314 - EX28DRAFT_0043 0.46 +0.4 23,558 + +0.4 23,558 + +0.3 23,559 - -0.6 23,559 - -2.0 23,569 - -1.5 23,569 - +0.8 23,596 + +0.2 23,614 - -1.5 23,723 + EX28DRAFT_0044 0.23 -0.3 23,723 + EX28DRAFT_0044 0.23 -0.5 23,724 - EX28DRAFT_0044 0.23 -2.2 23,724 - EX28DRAFT_0044 0.23 -2.4 23,758 + EX28DRAFT_0044 0.29 -0.0 23,759 - EX28DRAFT_0044 0.29 +0.9 23,808 + EX28DRAFT_0044 0.38 +0.0 23,872 + EX28DRAFT_0044 0.50 +0.3 23,872 + EX28DRAFT_0044 0.50 -0.1 23,873 - EX28DRAFT_0044 0.50 -3.8 23,873 - EX28DRAFT_0044 0.50 -0.7 23,940 + EX28DRAFT_0044 0.62 +0.6 23,963 - EX28DRAFT_0044 0.66 -1.6 23,963 - EX28DRAFT_0044 0.66 -1.3 24,058 - EX28DRAFT_0044 0.83 -0.4 24,063 + EX28DRAFT_0044 0.84 -1.0 24,107 - +1.4
Or see this region's nucleotide sequence