Experiment: PYE with CHIR-090 4 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_01450 and CCNA_R0036 are separated by 129 nucleotides CCNA_R0036 and CCNA_R0037 are separated by 45 nucleotides CCNA_R0037 and CCNA_01451 are separated by 172 nucleotides CCNA_01451 and CCNA_01452 overlap by 32 nucleotides
CCNA_01450: CCNA_01450 - single-stranded-DNA-specific exonuclease recJ, at 1,562,926 to 1,564,746
_01450
CCNA_R0036: CCNA_R0036 - tRNA-Glu, at 1,564,876 to 1,564,950
_R0036
CCNA_R0037: CCNA_R0037 - tRNA-Glu, at 1,564,996 to 1,565,070
_R0037
CCNA_01451: CCNA_01451 - cold shock protein cspD, at 1,565,243 to 1,565,833
_01451
CCNA_01452: CCNA_01452 - hypothetical protein, at 1,565,802 to 1,566,356
_01452
Position (kb)
1564
1565 Strain fitness (log2 ratio)
-1
0
1
2 at 1564.715 kb on + strand at 1564.721 kb on - strand at 1564.723 kb on - strand at 1564.749 kb on - strand at 1564.762 kb on + strand at 1564.771 kb on - strand at 1564.776 kb on - strand at 1564.943 kb on - strand at 1565.055 kb on + strand, within CCNA_R0037 at 1565.182 kb on - strand at 1565.228 kb on + strand at 1565.228 kb on + strand at 1565.228 kb on + strand at 1565.236 kb on - strand at 1565.236 kb on - strand at 1565.236 kb on - strand at 1565.236 kb on - strand at 1565.245 kb on - strand at 1565.330 kb on + strand, within CCNA_01451 at 1565.330 kb on + strand, within CCNA_01451 at 1565.332 kb on - strand, within CCNA_01451 at 1565.332 kb on - strand, within CCNA_01451 at 1565.338 kb on - strand, within CCNA_01451 at 1565.355 kb on + strand, within CCNA_01451 at 1565.363 kb on - strand, within CCNA_01451 at 1565.363 kb on - strand, within CCNA_01451 at 1565.363 kb on - strand, within CCNA_01451 at 1565.373 kb on - strand, within CCNA_01451 at 1565.447 kb on - strand, within CCNA_01451 at 1565.542 kb on + strand, within CCNA_01451 at 1565.609 kb on - strand, within CCNA_01451 at 1565.662 kb on + strand, within CCNA_01451 at 1565.663 kb on + strand, within CCNA_01451 at 1565.671 kb on - strand, within CCNA_01451 at 1565.671 kb on - strand, within CCNA_01451 at 1565.688 kb on - strand, within CCNA_01451 at 1565.713 kb on + strand, within CCNA_01451 at 1565.725 kb on + strand, within CCNA_01451 at 1565.773 kb on - strand, within CCNA_01451 at 1565.795 kb on - strand at 1565.855 kb on + strand at 1565.933 kb on - strand, within CCNA_01452
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with CHIR-090 4 ug/mL remove 1,564,715 + +0.2 1,564,721 - -0.8 1,564,723 - +0.5 1,564,749 - -0.5 1,564,762 + +2.3 1,564,771 - +0.5 1,564,776 - -1.2 1,564,943 - -0.7 1,565,055 + CCNA_R0037 0.79 +1.0 1,565,182 - -1.4 1,565,228 + +1.0 1,565,228 + -0.6 1,565,228 + +0.7 1,565,236 - +0.5 1,565,236 - -0.6 1,565,236 - -0.6 1,565,236 - -1.6 1,565,245 - -1.5 1,565,330 + CCNA_01451 0.15 -0.0 1,565,330 + CCNA_01451 0.15 +0.7 1,565,332 - CCNA_01451 0.15 -0.5 1,565,332 - CCNA_01451 0.15 +0.0 1,565,338 - CCNA_01451 0.16 -0.5 1,565,355 + CCNA_01451 0.19 +0.0 1,565,363 - CCNA_01451 0.20 -0.5 1,565,363 - CCNA_01451 0.20 -1.3 1,565,363 - CCNA_01451 0.20 -1.2 1,565,373 - CCNA_01451 0.22 -0.6 1,565,447 - CCNA_01451 0.35 -1.3 1,565,542 + CCNA_01451 0.51 -0.6 1,565,609 - CCNA_01451 0.62 -0.0 1,565,662 + CCNA_01451 0.71 +1.0 1,565,663 + CCNA_01451 0.71 +2.1 1,565,671 - CCNA_01451 0.72 +1.0 1,565,671 - CCNA_01451 0.72 -0.9 1,565,688 - CCNA_01451 0.75 -1.6 1,565,713 + CCNA_01451 0.80 +0.1 1,565,725 + CCNA_01451 0.82 +0.4 1,565,773 - CCNA_01451 0.90 -0.4 1,565,795 - -1.1 1,565,855 + +0.7 1,565,933 - CCNA_01452 0.24 +1.4
Or see this region's nucleotide sequence