Experiment: PYE with CHIR-090 4 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_03256 and CCNA_03990 are separated by 113 nucleotides CCNA_03990 and CCNA_03257 are separated by 27 nucleotides CCNA_03257 and CCNA_03258 are separated by 48 nucleotides CCNA_03258 and CCNA_03259 are separated by 11 nucleotides
CCNA_03256: CCNA_03256 - chorismate synthase, at 3,427,166 to 3,428,284
_03256
CCNA_03990: CCNA_03990 - hypothetical protein, at 3,428,398 to 3,428,430
_03990
CCNA_03257: CCNA_03257 - chemotaxis receiver domain protein CheYIII, at 3,428,458 to 3,429,000
_03257
CCNA_03258: CCNA_03258 - hypothetical protein, at 3,429,049 to 3,429,288
_03258
CCNA_03259: CCNA_03259 - mannose-6-phosphate isomerase, at 3,429,300 to 3,429,683
_03259
Position (kb)
3428
3429 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3427.405 kb on + strand, within CCNA_03256 at 3427.418 kb on + strand, within CCNA_03256 at 3427.486 kb on - strand, within CCNA_03256 at 3427.607 kb on + strand, within CCNA_03256 at 3427.623 kb on + strand, within CCNA_03256 at 3427.687 kb on - strand, within CCNA_03256 at 3427.689 kb on + strand, within CCNA_03256 at 3427.698 kb on - strand, within CCNA_03256 at 3427.719 kb on + strand, within CCNA_03256 at 3427.815 kb on + strand, within CCNA_03256 at 3427.843 kb on + strand, within CCNA_03256 at 3427.930 kb on - strand, within CCNA_03256 at 3427.946 kb on + strand, within CCNA_03256 at 3427.959 kb on - strand, within CCNA_03256 at 3428.004 kb on - strand, within CCNA_03256 at 3428.202 kb on - strand at 3428.286 kb on - strand at 3428.296 kb on - strand at 3428.356 kb on + strand at 3428.361 kb on + strand at 3428.361 kb on + strand at 3428.372 kb on + strand at 3428.470 kb on + strand at 3428.551 kb on - strand, within CCNA_03257 at 3428.620 kb on - strand, within CCNA_03257 at 3428.629 kb on + strand, within CCNA_03257 at 3428.635 kb on - strand, within CCNA_03257 at 3428.675 kb on - strand, within CCNA_03257 at 3428.690 kb on + strand, within CCNA_03257 at 3428.698 kb on - strand, within CCNA_03257 at 3428.786 kb on - strand, within CCNA_03257 at 3428.798 kb on - strand, within CCNA_03257 at 3428.809 kb on + strand, within CCNA_03257 at 3428.819 kb on + strand, within CCNA_03257 at 3428.827 kb on - strand, within CCNA_03257 at 3428.925 kb on - strand, within CCNA_03257 at 3428.936 kb on + strand, within CCNA_03257 at 3428.969 kb on - strand at 3429.003 kb on - strand at 3429.003 kb on - strand at 3429.011 kb on + strand at 3429.025 kb on + strand at 3429.047 kb on + strand at 3429.047 kb on + strand at 3429.077 kb on + strand, within CCNA_03258 at 3429.077 kb on + strand, within CCNA_03258 at 3429.081 kb on + strand, within CCNA_03258 at 3429.089 kb on - strand, within CCNA_03258 at 3429.106 kb on - strand, within CCNA_03258 at 3429.142 kb on + strand, within CCNA_03258 at 3429.206 kb on + strand, within CCNA_03258 at 3429.326 kb on + strand at 3429.349 kb on - strand, within CCNA_03259 at 3429.373 kb on + strand, within CCNA_03259 at 3429.374 kb on + strand, within CCNA_03259 at 3429.374 kb on + strand, within CCNA_03259 at 3429.374 kb on + strand, within CCNA_03259 at 3429.374 kb on + strand, within CCNA_03259 at 3429.382 kb on - strand, within CCNA_03259 at 3429.382 kb on - strand, within CCNA_03259 at 3429.382 kb on - strand, within CCNA_03259
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with CHIR-090 4 ug/mL remove 3,427,405 + CCNA_03256 0.21 +0.3 3,427,418 + CCNA_03256 0.23 -0.5 3,427,486 - CCNA_03256 0.29 -0.0 3,427,607 + CCNA_03256 0.39 +1.4 3,427,623 + CCNA_03256 0.41 +1.4 3,427,687 - CCNA_03256 0.47 +1.1 3,427,689 + CCNA_03256 0.47 -0.2 3,427,698 - CCNA_03256 0.48 -1.2 3,427,719 + CCNA_03256 0.49 +1.9 3,427,815 + CCNA_03256 0.58 +0.7 3,427,843 + CCNA_03256 0.61 -1.2 3,427,930 - CCNA_03256 0.68 +0.8 3,427,946 + CCNA_03256 0.70 -0.6 3,427,959 - CCNA_03256 0.71 +0.7 3,428,004 - CCNA_03256 0.75 +1.4 3,428,202 - -1.1 3,428,286 - +0.4 3,428,296 - -0.3 3,428,356 + +0.2 3,428,361 + +0.5 3,428,361 + +0.9 3,428,372 + +1.0 3,428,470 + +0.2 3,428,551 - CCNA_03257 0.17 -0.3 3,428,620 - CCNA_03257 0.30 +0.4 3,428,629 + CCNA_03257 0.31 +0.0 3,428,635 - CCNA_03257 0.33 +0.1 3,428,675 - CCNA_03257 0.40 -0.6 3,428,690 + CCNA_03257 0.43 +1.0 3,428,698 - CCNA_03257 0.44 +0.0 3,428,786 - CCNA_03257 0.60 +0.3 3,428,798 - CCNA_03257 0.63 -0.4 3,428,809 + CCNA_03257 0.65 +0.7 3,428,819 + CCNA_03257 0.66 +0.0 3,428,827 - CCNA_03257 0.68 -2.2 3,428,925 - CCNA_03257 0.86 -0.6 3,428,936 + CCNA_03257 0.88 -0.2 3,428,969 - +0.6 3,429,003 - -0.6 3,429,003 - -2.7 3,429,011 + +0.2 3,429,025 + -0.0 3,429,047 + -0.2 3,429,047 + +0.3 3,429,077 + CCNA_03258 0.12 +0.6 3,429,077 + CCNA_03258 0.12 +0.4 3,429,081 + CCNA_03258 0.13 -0.3 3,429,089 - CCNA_03258 0.17 -0.2 3,429,106 - CCNA_03258 0.24 -0.7 3,429,142 + CCNA_03258 0.39 -1.0 3,429,206 + CCNA_03258 0.65 -1.0 3,429,326 + +0.1 3,429,349 - CCNA_03259 0.13 -0.3 3,429,373 + CCNA_03259 0.19 +0.7 3,429,374 + CCNA_03259 0.19 +0.1 3,429,374 + CCNA_03259 0.19 +0.2 3,429,374 + CCNA_03259 0.19 +0.5 3,429,374 + CCNA_03259 0.19 +0.1 3,429,382 - CCNA_03259 0.21 +1.0 3,429,382 - CCNA_03259 0.21 +0.9 3,429,382 - CCNA_03259 0.21 +0.1
Or see this region's nucleotide sequence