Experiment: PYE with CHIR-090 4 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_02961 and CCNA_03983 are separated by 123 nucleotides CCNA_03983 and CCNA_02962 overlap by 11 nucleotides CCNA_02962 and CCNA_02963 are separated by 64 nucleotides CCNA_02963 and CCNA_03984 are separated by 24 nucleotides CCNA_03984 and CCNA_02964 are separated by 60 nucleotides
CCNA_02961: CCNA_02961 - NeuB-family N-acetylneuraminate synthase, at 3,117,581 to 3,118,651
_02961
CCNA_03983: CCNA_03983 - HicA-related toxin-antitoxin protein, at 3,118,775 to 3,119,035
_03983
CCNA_02962: CCNA_02962 - HicB-family RNAse H fold protein, at 3,119,025 to 3,119,252
_02962
CCNA_02963: CCNA_02963 - hypothetical protein, at 3,119,317 to 3,119,577
_02963
CCNA_03984: CCNA_03984 - hypothetical protein, at 3,119,602 to 3,119,790
_03984
CCNA_02964: CCNA_02964 - thioredoxin-disulfide reductase, at 3,119,851 to 3,120,858
_02964
Position (kb)
3119
3120 Strain fitness (log2 ratio)
-2
-1
0
1 at 3118.030 kb on - strand, within CCNA_02961 at 3118.032 kb on + strand, within CCNA_02961 at 3118.040 kb on - strand, within CCNA_02961 at 3118.068 kb on + strand, within CCNA_02961 at 3118.075 kb on - strand, within CCNA_02961 at 3118.109 kb on + strand, within CCNA_02961 at 3118.109 kb on + strand, within CCNA_02961 at 3118.117 kb on - strand, within CCNA_02961 at 3118.119 kb on - strand, within CCNA_02961 at 3118.121 kb on + strand, within CCNA_02961 at 3118.124 kb on - strand, within CCNA_02961 at 3118.129 kb on - strand, within CCNA_02961 at 3118.184 kb on - strand, within CCNA_02961 at 3118.205 kb on - strand, within CCNA_02961 at 3118.236 kb on + strand, within CCNA_02961 at 3118.238 kb on + strand, within CCNA_02961 at 3118.252 kb on + strand, within CCNA_02961 at 3118.262 kb on + strand, within CCNA_02961 at 3118.302 kb on - strand, within CCNA_02961 at 3118.360 kb on + strand, within CCNA_02961 at 3118.362 kb on + strand, within CCNA_02961 at 3118.362 kb on + strand, within CCNA_02961 at 3118.369 kb on - strand, within CCNA_02961 at 3118.370 kb on - strand, within CCNA_02961 at 3118.379 kb on - strand, within CCNA_02961 at 3118.392 kb on + strand, within CCNA_02961 at 3118.400 kb on + strand, within CCNA_02961 at 3118.409 kb on - strand, within CCNA_02961 at 3118.455 kb on - strand, within CCNA_02961 at 3118.472 kb on - strand, within CCNA_02961 at 3118.477 kb on - strand, within CCNA_02961 at 3118.477 kb on - strand, within CCNA_02961 at 3118.477 kb on - strand, within CCNA_02961 at 3118.539 kb on - strand, within CCNA_02961 at 3118.539 kb on - strand, within CCNA_02961 at 3118.542 kb on + strand, within CCNA_02961 at 3118.551 kb on + strand at 3118.569 kb on - strand at 3118.642 kb on + strand at 3118.654 kb on - strand at 3118.671 kb on - strand at 3118.750 kb on + strand at 3118.777 kb on + strand at 3118.851 kb on - strand, within CCNA_03983 at 3118.854 kb on + strand, within CCNA_03983 at 3118.868 kb on + strand, within CCNA_03983 at 3118.870 kb on - strand, within CCNA_03983 at 3118.899 kb on + strand, within CCNA_03983 at 3118.914 kb on - strand, within CCNA_03983 at 3118.985 kb on - strand, within CCNA_03983 at 3119.039 kb on + strand at 3119.188 kb on + strand, within CCNA_02962 at 3119.245 kb on + strand at 3119.277 kb on - strand at 3119.283 kb on - strand at 3119.328 kb on + strand at 3119.366 kb on - strand, within CCNA_02963 at 3119.484 kb on + strand, within CCNA_02963 at 3119.487 kb on - strand, within CCNA_02963 at 3119.492 kb on - strand, within CCNA_02963 at 3119.492 kb on - strand, within CCNA_02963 at 3119.496 kb on + strand, within CCNA_02963 at 3119.542 kb on - strand, within CCNA_02963 at 3119.636 kb on - strand, within CCNA_03984 at 3119.643 kb on + strand, within CCNA_03984 at 3119.707 kb on + strand, within CCNA_03984 at 3119.715 kb on - strand, within CCNA_03984 at 3119.751 kb on + strand, within CCNA_03984 at 3119.767 kb on + strand, within CCNA_03984 at 3119.769 kb on - strand, within CCNA_03984 at 3119.772 kb on + strand at 3119.777 kb on + strand at 3119.835 kb on + strand at 3119.837 kb on + strand at 3119.852 kb on + strand at 3119.852 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with CHIR-090 4 ug/mL remove 3,118,030 - CCNA_02961 0.42 +0.8 3,118,032 + CCNA_02961 0.42 -0.6 3,118,040 - CCNA_02961 0.43 +0.5 3,118,068 + CCNA_02961 0.45 +0.1 3,118,075 - CCNA_02961 0.46 +0.6 3,118,109 + CCNA_02961 0.49 +0.4 3,118,109 + CCNA_02961 0.49 -2.0 3,118,117 - CCNA_02961 0.50 +0.9 3,118,119 - CCNA_02961 0.50 -0.3 3,118,121 + CCNA_02961 0.50 -0.8 3,118,124 - CCNA_02961 0.51 +0.1 3,118,129 - CCNA_02961 0.51 +0.2 3,118,184 - CCNA_02961 0.56 +0.7 3,118,205 - CCNA_02961 0.58 +0.5 3,118,236 + CCNA_02961 0.61 +0.0 3,118,238 + CCNA_02961 0.61 -1.3 3,118,252 + CCNA_02961 0.63 -0.2 3,118,262 + CCNA_02961 0.64 +0.9 3,118,302 - CCNA_02961 0.67 -0.8 3,118,360 + CCNA_02961 0.73 -0.9 3,118,362 + CCNA_02961 0.73 +0.3 3,118,362 + CCNA_02961 0.73 +0.0 3,118,369 - CCNA_02961 0.74 +0.1 3,118,370 - CCNA_02961 0.74 +0.4 3,118,379 - CCNA_02961 0.75 -0.2 3,118,392 + CCNA_02961 0.76 -1.5 3,118,400 + CCNA_02961 0.76 -1.0 3,118,409 - CCNA_02961 0.77 -1.0 3,118,455 - CCNA_02961 0.82 -0.9 3,118,472 - CCNA_02961 0.83 -0.7 3,118,477 - CCNA_02961 0.84 -0.5 3,118,477 - CCNA_02961 0.84 -1.8 3,118,477 - CCNA_02961 0.84 -1.2 3,118,539 - CCNA_02961 0.89 -0.3 3,118,539 - CCNA_02961 0.89 +0.9 3,118,542 + CCNA_02961 0.90 +0.4 3,118,551 + -0.9 3,118,569 - -0.1 3,118,642 + -0.2 3,118,654 - +0.1 3,118,671 - -0.1 3,118,750 + +0.6 3,118,777 + +1.6 3,118,851 - CCNA_03983 0.29 -1.0 3,118,854 + CCNA_03983 0.30 +0.4 3,118,868 + CCNA_03983 0.36 -0.9 3,118,870 - CCNA_03983 0.36 -0.9 3,118,899 + CCNA_03983 0.48 +0.3 3,118,914 - CCNA_03983 0.53 +0.9 3,118,985 - CCNA_03983 0.80 -0.6 3,119,039 + -0.4 3,119,188 + CCNA_02962 0.71 -2.0 3,119,245 + +0.0 3,119,277 - -0.1 3,119,283 - +0.1 3,119,328 + -0.2 3,119,366 - CCNA_02963 0.19 -0.1 3,119,484 + CCNA_02963 0.64 -1.4 3,119,487 - CCNA_02963 0.65 +0.5 3,119,492 - CCNA_02963 0.67 -0.7 3,119,492 - CCNA_02963 0.67 +1.4 3,119,496 + CCNA_02963 0.69 -0.6 3,119,542 - CCNA_02963 0.86 +0.2 3,119,636 - CCNA_03984 0.18 +0.0 3,119,643 + CCNA_03984 0.22 -0.0 3,119,707 + CCNA_03984 0.56 +0.7 3,119,715 - CCNA_03984 0.60 +0.1 3,119,751 + CCNA_03984 0.79 -0.8 3,119,767 + CCNA_03984 0.87 -1.3 3,119,769 - CCNA_03984 0.88 +0.4 3,119,772 + +1.4 3,119,777 + -0.2 3,119,835 + +0.3 3,119,837 + +1.0 3,119,852 + -0.6 3,119,852 + -0.5
Or see this region's nucleotide sequence