Experiment: PYE with CHIR-090 4 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_02846 and CCNA_02847 are separated by 179 nucleotides CCNA_02847 and CCNA_02848 overlap by 4 nucleotides
CCNA_02846: CCNA_02846 - DegP/HtrA-family serine protease, at 2,998,630 to 3,000,222
_02846
CCNA_02847: CCNA_02847 - cytochrome c-type biogenesis protein ccmH, at 3,000,402 to 3,000,854
_02847
CCNA_02848: CCNA_02848 - heme chaperone-apocytochrome heme-lyase, at 3,000,851 to 3,002,833
_02848
Position (kb)
3000
3001 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2999.467 kb on + strand, within CCNA_02846 at 2999.482 kb on + strand, within CCNA_02846 at 2999.508 kb on + strand, within CCNA_02846 at 2999.521 kb on - strand, within CCNA_02846 at 2999.566 kb on + strand, within CCNA_02846 at 2999.571 kb on + strand, within CCNA_02846 at 2999.642 kb on + strand, within CCNA_02846 at 2999.650 kb on + strand, within CCNA_02846 at 2999.652 kb on - strand, within CCNA_02846 at 2999.657 kb on + strand, within CCNA_02846 at 2999.657 kb on + strand, within CCNA_02846 at 2999.659 kb on - strand, within CCNA_02846 at 2999.663 kb on + strand, within CCNA_02846 at 2999.677 kb on - strand, within CCNA_02846 at 2999.696 kb on - strand, within CCNA_02846 at 2999.702 kb on - strand, within CCNA_02846 at 2999.714 kb on - strand, within CCNA_02846 at 2999.721 kb on + strand, within CCNA_02846 at 2999.754 kb on - strand, within CCNA_02846 at 2999.756 kb on + strand, within CCNA_02846 at 2999.807 kb on - strand, within CCNA_02846 at 2999.952 kb on + strand, within CCNA_02846 at 2999.952 kb on + strand, within CCNA_02846 at 3000.001 kb on - strand, within CCNA_02846 at 3000.006 kb on - strand, within CCNA_02846 at 3000.006 kb on - strand, within CCNA_02846 at 3000.104 kb on + strand at 3000.117 kb on - strand at 3000.164 kb on - strand at 3000.259 kb on + strand at 3000.267 kb on - strand at 3000.267 kb on - strand at 3000.290 kb on + strand at 3000.329 kb on - strand at 3000.329 kb on - strand at 3000.417 kb on - strand at 3000.419 kb on + strand at 3000.634 kb on + strand, within CCNA_02847 at 3000.642 kb on - strand, within CCNA_02847 at 3000.642 kb on - strand, within CCNA_02847 at 3000.642 kb on - strand, within CCNA_02847 at 3000.642 kb on - strand, within CCNA_02847 at 3000.642 kb on - strand, within CCNA_02847 at 3000.642 kb on - strand, within CCNA_02847 at 3000.730 kb on + strand, within CCNA_02847 at 3000.738 kb on - strand, within CCNA_02847 at 3000.738 kb on - strand, within CCNA_02847 at 3000.738 kb on - strand, within CCNA_02847 at 3000.738 kb on - strand, within CCNA_02847 at 3000.738 kb on - strand, within CCNA_02847 at 3000.738 kb on - strand, within CCNA_02847 at 3000.738 kb on - strand, within CCNA_02847 at 3000.738 kb on - strand, within CCNA_02847 at 3000.738 kb on - strand, within CCNA_02847 at 3000.738 kb on - strand, within CCNA_02847 at 3000.740 kb on - strand, within CCNA_02847 at 3000.836 kb on - strand at 3000.836 kb on - strand at 3000.836 kb on - strand at 3000.846 kb on - strand at 3001.007 kb on + strand at 3001.030 kb on - strand at 3001.345 kb on - strand, within CCNA_02848 at 3001.421 kb on - strand, within CCNA_02848 at 3001.492 kb on - strand, within CCNA_02848 at 3001.697 kb on - strand, within CCNA_02848 at 3001.705 kb on - strand, within CCNA_02848
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with CHIR-090 4 ug/mL remove 2,999,467 + CCNA_02846 0.53 -1.6 2,999,482 + CCNA_02846 0.53 +0.2 2,999,508 + CCNA_02846 0.55 -0.1 2,999,521 - CCNA_02846 0.56 +0.7 2,999,566 + CCNA_02846 0.59 -1.2 2,999,571 + CCNA_02846 0.59 -0.0 2,999,642 + CCNA_02846 0.64 +0.2 2,999,650 + CCNA_02846 0.64 -1.6 2,999,652 - CCNA_02846 0.64 +1.1 2,999,657 + CCNA_02846 0.64 -0.0 2,999,657 + CCNA_02846 0.64 -1.7 2,999,659 - CCNA_02846 0.65 +0.4 2,999,663 + CCNA_02846 0.65 -0.2 2,999,677 - CCNA_02846 0.66 +0.5 2,999,696 - CCNA_02846 0.67 +0.7 2,999,702 - CCNA_02846 0.67 +0.7 2,999,714 - CCNA_02846 0.68 -0.4 2,999,721 + CCNA_02846 0.68 +2.6 2,999,754 - CCNA_02846 0.71 -0.1 2,999,756 + CCNA_02846 0.71 -1.6 2,999,807 - CCNA_02846 0.74 -0.1 2,999,952 + CCNA_02846 0.83 -0.0 2,999,952 + CCNA_02846 0.83 +0.6 3,000,001 - CCNA_02846 0.86 -1.0 3,000,006 - CCNA_02846 0.86 +0.7 3,000,006 - CCNA_02846 0.86 +0.2 3,000,104 + +0.4 3,000,117 - +0.3 3,000,164 - +0.1 3,000,259 + -2.3 3,000,267 - +0.2 3,000,267 - +0.2 3,000,290 + -0.9 3,000,329 - +0.4 3,000,329 - +0.1 3,000,417 - +0.5 3,000,419 + -1.6 3,000,634 + CCNA_02847 0.51 -1.8 3,000,642 - CCNA_02847 0.53 -0.8 3,000,642 - CCNA_02847 0.53 -0.0 3,000,642 - CCNA_02847 0.53 +0.8 3,000,642 - CCNA_02847 0.53 -0.5 3,000,642 - CCNA_02847 0.53 +0.9 3,000,642 - CCNA_02847 0.53 -1.1 3,000,730 + CCNA_02847 0.72 +0.6 3,000,738 - CCNA_02847 0.74 +1.0 3,000,738 - CCNA_02847 0.74 +0.2 3,000,738 - CCNA_02847 0.74 -1.0 3,000,738 - CCNA_02847 0.74 -1.4 3,000,738 - CCNA_02847 0.74 -1.1 3,000,738 - CCNA_02847 0.74 -0.3 3,000,738 - CCNA_02847 0.74 +1.8 3,000,738 - CCNA_02847 0.74 +0.6 3,000,738 - CCNA_02847 0.74 +0.3 3,000,738 - CCNA_02847 0.74 -0.2 3,000,740 - CCNA_02847 0.75 -0.3 3,000,836 - -1.4 3,000,836 - +0.8 3,000,836 - -1.8 3,000,846 - +0.3 3,001,007 + -0.9 3,001,030 - +0.6 3,001,345 - CCNA_02848 0.25 -1.1 3,001,421 - CCNA_02848 0.29 -0.1 3,001,492 - CCNA_02848 0.32 -0.9 3,001,697 - CCNA_02848 0.43 +0.8 3,001,705 - CCNA_02848 0.43 -0.8
Or see this region's nucleotide sequence