Experiment: PYE with CHIR-090 4 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_02386 and CCNA_02387 overlap by 4 nucleotides CCNA_02387 and CCNA_02388 are separated by 42 nucleotides CCNA_02388 and CCNA_02389 overlap by 4 nucleotides
CCNA_02386: CCNA_02386 - O-antigen ligase related enzyme, at 2,529,502 to 2,530,725
_02386
CCNA_02387: CCNA_02387 - glutathione reductase, at 2,530,722 to 2,532,122
_02387
CCNA_02388: CCNA_02388 - ribose 5-phosphate isomerase, at 2,532,165 to 2,532,848
_02388
CCNA_02389: CCNA_02389 - glucosamine-1-phosphate acetyltransferase/UDP-N-acetylglucosamine pyrophosphorylase, at 2,532,845 to 2,534,233
_02389
Position (kb)
2530
2531
2532
2533 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2529.743 kb on + strand, within CCNA_02386 at 2529.769 kb on - strand, within CCNA_02386 at 2529.770 kb on + strand, within CCNA_02386 at 2529.828 kb on - strand, within CCNA_02386 at 2529.899 kb on + strand, within CCNA_02386 at 2529.935 kb on + strand, within CCNA_02386 at 2529.959 kb on + strand, within CCNA_02386 at 2530.084 kb on - strand, within CCNA_02386 at 2530.086 kb on - strand, within CCNA_02386 at 2530.180 kb on - strand, within CCNA_02386 at 2530.212 kb on + strand, within CCNA_02386 at 2530.227 kb on + strand, within CCNA_02386 at 2530.266 kb on - strand, within CCNA_02386 at 2530.335 kb on + strand, within CCNA_02386 at 2530.343 kb on - strand, within CCNA_02386 at 2530.343 kb on - strand, within CCNA_02386 at 2530.343 kb on - strand, within CCNA_02386 at 2530.343 kb on - strand, within CCNA_02386 at 2530.343 kb on - strand, within CCNA_02386 at 2530.405 kb on - strand, within CCNA_02386 at 2530.433 kb on - strand, within CCNA_02386 at 2530.434 kb on - strand, within CCNA_02386 at 2530.603 kb on + strand at 2530.611 kb on - strand at 2530.679 kb on - strand at 2530.724 kb on + strand at 2530.981 kb on + strand, within CCNA_02387 at 2531.710 kb on + strand, within CCNA_02387 at 2531.718 kb on - strand, within CCNA_02387 at 2532.150 kb on + strand at 2532.158 kb on - strand at 2532.163 kb on + strand at 2532.163 kb on + strand at 2532.171 kb on - strand at 2532.180 kb on + strand at 2532.194 kb on - strand at 2532.195 kb on - strand at 2532.403 kb on - strand, within CCNA_02388
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with CHIR-090 4 ug/mL remove 2,529,743 + CCNA_02386 0.20 -2.4 2,529,769 - CCNA_02386 0.22 +0.1 2,529,770 + CCNA_02386 0.22 -0.4 2,529,828 - CCNA_02386 0.27 -1.5 2,529,899 + CCNA_02386 0.32 -0.2 2,529,935 + CCNA_02386 0.35 +0.5 2,529,959 + CCNA_02386 0.37 +0.5 2,530,084 - CCNA_02386 0.48 +1.1 2,530,086 - CCNA_02386 0.48 -0.1 2,530,180 - CCNA_02386 0.55 -1.5 2,530,212 + CCNA_02386 0.58 -0.3 2,530,227 + CCNA_02386 0.59 +0.5 2,530,266 - CCNA_02386 0.62 -0.7 2,530,335 + CCNA_02386 0.68 -0.3 2,530,343 - CCNA_02386 0.69 -2.4 2,530,343 - CCNA_02386 0.69 +0.2 2,530,343 - CCNA_02386 0.69 +0.0 2,530,343 - CCNA_02386 0.69 +0.2 2,530,343 - CCNA_02386 0.69 -0.1 2,530,405 - CCNA_02386 0.74 -0.4 2,530,433 - CCNA_02386 0.76 +1.8 2,530,434 - CCNA_02386 0.76 -0.1 2,530,603 + -2.5 2,530,611 - +0.6 2,530,679 - -0.1 2,530,724 + +0.1 2,530,981 + CCNA_02387 0.18 +0.5 2,531,710 + CCNA_02387 0.71 +0.2 2,531,718 - CCNA_02387 0.71 -1.1 2,532,150 + +1.2 2,532,158 - +0.3 2,532,163 + -1.0 2,532,163 + +1.1 2,532,171 - -0.1 2,532,180 + -0.7 2,532,194 - +0.2 2,532,195 - +1.2 2,532,403 - CCNA_02388 0.35 +1.4
Or see this region's nucleotide sequence