Experiment: PYE with CHIR-090 4 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_02296 and CCNA_02297 are separated by 138 nucleotides CCNA_02297 and CCNA_02298 overlap by 59 nucleotides CCNA_02298 and CCNA_02299 are separated by 58 nucleotides CCNA_02299 and CCNA_02300 overlap by 4 nucleotides
CCNA_02296: CCNA_02296 - diaminopimelate decarboxylase, at 2,442,876 to 2,444,141
_02296
CCNA_02297: CCNA_02297 - hypoxanthine-guanine phosphoribosyltransferase, at 2,444,280 to 2,444,861
_02297
CCNA_02298: CCNA_02298 - MJ0042 family finger-like domain protein, at 2,444,803 to 2,445,762
_02298
CCNA_02299: CCNA_02299 - cell division ATP-binding protein FtsE, at 2,445,821 to 2,446,528
_02299
CCNA_02300: CCNA_02300 - cell division protein FtsX, at 2,446,525 to 2,447,418
_02300
Position (kb)
2444
2445
2446 Strain fitness (log2 ratio)
-1
0
1 at 2444.215 kb on + strand at 2444.222 kb on - strand at 2444.222 kb on - strand at 2444.278 kb on + strand at 2444.278 kb on + strand at 2444.304 kb on - strand at 2444.316 kb on - strand at 2444.333 kb on - strand at 2444.400 kb on - strand, within CCNA_02297 at 2444.407 kb on - strand, within CCNA_02297 at 2444.417 kb on - strand, within CCNA_02297 at 2444.438 kb on - strand, within CCNA_02297 at 2444.442 kb on + strand, within CCNA_02297 at 2444.455 kb on + strand, within CCNA_02297 at 2444.473 kb on + strand, within CCNA_02297 at 2444.473 kb on + strand, within CCNA_02297 at 2444.481 kb on - strand, within CCNA_02297 at 2444.484 kb on + strand, within CCNA_02297 at 2444.489 kb on + strand, within CCNA_02297 at 2444.571 kb on + strand, within CCNA_02297 at 2444.633 kb on - strand, within CCNA_02297 at 2444.646 kb on - strand, within CCNA_02297 at 2444.697 kb on + strand, within CCNA_02297 at 2444.697 kb on + strand, within CCNA_02297 at 2444.778 kb on + strand, within CCNA_02297 at 2444.838 kb on - strand at 2444.903 kb on - strand, within CCNA_02298 at 2444.935 kb on - strand, within CCNA_02298 at 2444.942 kb on - strand, within CCNA_02298 at 2444.954 kb on + strand, within CCNA_02298 at 2444.969 kb on + strand, within CCNA_02298 at 2444.976 kb on - strand, within CCNA_02298 at 2445.018 kb on + strand, within CCNA_02298 at 2445.018 kb on + strand, within CCNA_02298 at 2445.021 kb on + strand, within CCNA_02298 at 2445.027 kb on - strand, within CCNA_02298 at 2445.029 kb on - strand, within CCNA_02298 at 2445.029 kb on - strand, within CCNA_02298 at 2445.072 kb on - strand, within CCNA_02298 at 2445.171 kb on + strand, within CCNA_02298 at 2445.178 kb on - strand, within CCNA_02298 at 2445.216 kb on - strand, within CCNA_02298 at 2445.226 kb on + strand, within CCNA_02298 at 2445.238 kb on + strand, within CCNA_02298 at 2445.245 kb on + strand, within CCNA_02298 at 2445.281 kb on - strand, within CCNA_02298 at 2445.303 kb on - strand, within CCNA_02298 at 2445.322 kb on + strand, within CCNA_02298 at 2445.355 kb on + strand, within CCNA_02298 at 2445.363 kb on - strand, within CCNA_02298 at 2445.383 kb on - strand, within CCNA_02298 at 2445.409 kb on - strand, within CCNA_02298 at 2445.409 kb on - strand, within CCNA_02298 at 2445.454 kb on - strand, within CCNA_02298 at 2445.461 kb on + strand, within CCNA_02298 at 2445.484 kb on + strand, within CCNA_02298 at 2445.571 kb on - strand, within CCNA_02298 at 2445.609 kb on + strand, within CCNA_02298 at 2445.640 kb on - strand, within CCNA_02298 at 2445.683 kb on + strand at 2445.683 kb on + strand at 2445.684 kb on + strand at 2445.691 kb on - strand at 2445.727 kb on + strand at 2445.735 kb on - strand at 2445.806 kb on + strand at 2445.836 kb on + strand at 2445.836 kb on + strand at 2445.841 kb on + strand at 2445.849 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with CHIR-090 4 ug/mL remove 2,444,215 + +0.0 2,444,222 - +0.8 2,444,222 - -0.4 2,444,278 + +1.6 2,444,278 + +0.5 2,444,304 - +1.2 2,444,316 - -0.5 2,444,333 - +0.3 2,444,400 - CCNA_02297 0.21 +0.4 2,444,407 - CCNA_02297 0.22 +0.1 2,444,417 - CCNA_02297 0.24 +0.4 2,444,438 - CCNA_02297 0.27 +0.4 2,444,442 + CCNA_02297 0.28 +0.2 2,444,455 + CCNA_02297 0.30 -0.0 2,444,473 + CCNA_02297 0.33 +0.1 2,444,473 + CCNA_02297 0.33 -0.6 2,444,481 - CCNA_02297 0.35 -0.2 2,444,484 + CCNA_02297 0.35 -0.1 2,444,489 + CCNA_02297 0.36 -0.2 2,444,571 + CCNA_02297 0.50 -0.3 2,444,633 - CCNA_02297 0.61 +0.4 2,444,646 - CCNA_02297 0.63 +0.6 2,444,697 + CCNA_02297 0.72 -1.1 2,444,697 + CCNA_02297 0.72 +1.5 2,444,778 + CCNA_02297 0.86 +0.0 2,444,838 - +0.3 2,444,903 - CCNA_02298 0.10 -0.2 2,444,935 - CCNA_02298 0.14 -0.0 2,444,942 - CCNA_02298 0.14 +1.0 2,444,954 + CCNA_02298 0.16 +0.1 2,444,969 + CCNA_02298 0.17 -1.2 2,444,976 - CCNA_02298 0.18 -0.0 2,445,018 + CCNA_02298 0.22 +0.3 2,445,018 + CCNA_02298 0.22 -0.0 2,445,021 + CCNA_02298 0.23 +0.7 2,445,027 - CCNA_02298 0.23 +0.7 2,445,029 - CCNA_02298 0.24 -0.7 2,445,029 - CCNA_02298 0.24 +0.4 2,445,072 - CCNA_02298 0.28 +1.0 2,445,171 + CCNA_02298 0.38 -0.8 2,445,178 - CCNA_02298 0.39 +0.6 2,445,216 - CCNA_02298 0.43 +0.8 2,445,226 + CCNA_02298 0.44 -1.1 2,445,238 + CCNA_02298 0.45 -0.6 2,445,245 + CCNA_02298 0.46 -0.4 2,445,281 - CCNA_02298 0.50 +0.4 2,445,303 - CCNA_02298 0.52 +0.0 2,445,322 + CCNA_02298 0.54 -0.4 2,445,355 + CCNA_02298 0.57 -1.1 2,445,363 - CCNA_02298 0.58 +0.5 2,445,383 - CCNA_02298 0.60 +0.2 2,445,409 - CCNA_02298 0.63 +0.5 2,445,409 - CCNA_02298 0.63 +0.3 2,445,454 - CCNA_02298 0.68 +0.9 2,445,461 + CCNA_02298 0.69 -0.3 2,445,484 + CCNA_02298 0.71 +0.3 2,445,571 - CCNA_02298 0.80 +0.1 2,445,609 + CCNA_02298 0.84 -0.2 2,445,640 - CCNA_02298 0.87 +1.0 2,445,683 + -1.5 2,445,683 + -0.2 2,445,684 + +0.3 2,445,691 - +0.0 2,445,727 + -0.4 2,445,735 - +0.2 2,445,806 + -0.7 2,445,836 + -1.1 2,445,836 + -0.6 2,445,841 + +0.0 2,445,849 - +0.6
Or see this region's nucleotide sequence