Experiment: PYE with CHIR-090 4 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_01948 and CCNA_01949 overlap by 20 nucleotides CCNA_01949 and CCNA_01950 are separated by 77 nucleotides
CCNA_01948: CCNA_01948 - peptidoglycan-specific endopeptidase, M23 family, at 2,090,363 to 2,091,514
_01948
CCNA_01949: CCNA_01949 - bactofilin A BacA, at 2,091,495 to 2,091,980
_01949
CCNA_01950: CCNA_01950 - peptide chain Release factor 2 (RF-2), at 2,092,058 to 2,093,173
_01950
Position (kb)
2091
2092 Strain fitness (log2 ratio)
-1
0
1
2 at 2090.526 kb on - strand, within CCNA_01948 at 2090.548 kb on + strand, within CCNA_01948 at 2090.614 kb on + strand, within CCNA_01948 at 2090.614 kb on + strand, within CCNA_01948 at 2090.636 kb on + strand, within CCNA_01948 at 2090.636 kb on + strand, within CCNA_01948 at 2090.644 kb on - strand, within CCNA_01948 at 2090.644 kb on - strand, within CCNA_01948 at 2090.644 kb on - strand, within CCNA_01948 at 2090.659 kb on + strand, within CCNA_01948 at 2090.791 kb on - strand, within CCNA_01948 at 2090.816 kb on - strand, within CCNA_01948 at 2090.829 kb on - strand, within CCNA_01948 at 2090.840 kb on + strand, within CCNA_01948 at 2090.840 kb on + strand, within CCNA_01948 at 2090.840 kb on + strand, within CCNA_01948 at 2090.849 kb on + strand, within CCNA_01948 at 2090.864 kb on + strand, within CCNA_01948 at 2090.864 kb on + strand, within CCNA_01948 at 2090.884 kb on - strand, within CCNA_01948 at 2090.889 kb on - strand, within CCNA_01948 at 2091.008 kb on + strand, within CCNA_01948 at 2091.068 kb on - strand, within CCNA_01948 at 2091.197 kb on - strand, within CCNA_01948 at 2091.216 kb on + strand, within CCNA_01948 at 2091.224 kb on - strand, within CCNA_01948 at 2091.278 kb on - strand, within CCNA_01948 at 2091.356 kb on - strand, within CCNA_01948 at 2091.405 kb on - strand at 2091.458 kb on + strand at 2091.458 kb on + strand at 2091.466 kb on - strand at 2091.536 kb on - strand at 2091.599 kb on + strand, within CCNA_01949 at 2091.607 kb on - strand, within CCNA_01949 at 2091.622 kb on + strand, within CCNA_01949 at 2091.622 kb on + strand, within CCNA_01949 at 2091.732 kb on - strand, within CCNA_01949 at 2091.742 kb on - strand, within CCNA_01949 at 2091.759 kb on - strand, within CCNA_01949 at 2091.895 kb on - strand, within CCNA_01949 at 2091.975 kb on + strand at 2091.993 kb on - strand at 2092.008 kb on + strand at 2092.055 kb on + strand at 2092.082 kb on + strand at 2092.082 kb on + strand at 2092.090 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with CHIR-090 4 ug/mL remove 2,090,526 - CCNA_01948 0.14 -0.1 2,090,548 + CCNA_01948 0.16 +0.2 2,090,614 + CCNA_01948 0.22 +1.0 2,090,614 + CCNA_01948 0.22 +0.7 2,090,636 + CCNA_01948 0.24 +0.5 2,090,636 + CCNA_01948 0.24 +0.4 2,090,644 - CCNA_01948 0.24 -0.2 2,090,644 - CCNA_01948 0.24 -0.3 2,090,644 - CCNA_01948 0.24 -0.5 2,090,659 + CCNA_01948 0.26 +0.4 2,090,791 - CCNA_01948 0.37 -1.0 2,090,816 - CCNA_01948 0.39 -0.6 2,090,829 - CCNA_01948 0.40 -1.6 2,090,840 + CCNA_01948 0.41 +1.7 2,090,840 + CCNA_01948 0.41 +0.6 2,090,840 + CCNA_01948 0.41 +1.7 2,090,849 + CCNA_01948 0.42 +0.4 2,090,864 + CCNA_01948 0.43 +0.1 2,090,864 + CCNA_01948 0.43 +0.1 2,090,884 - CCNA_01948 0.45 +1.2 2,090,889 - CCNA_01948 0.46 -0.1 2,091,008 + CCNA_01948 0.56 +0.7 2,091,068 - CCNA_01948 0.61 -0.7 2,091,197 - CCNA_01948 0.72 +0.8 2,091,216 + CCNA_01948 0.74 +0.7 2,091,224 - CCNA_01948 0.75 +0.5 2,091,278 - CCNA_01948 0.79 +0.2 2,091,356 - CCNA_01948 0.86 -0.8 2,091,405 - -0.3 2,091,458 + +0.1 2,091,458 + +0.1 2,091,466 - +0.2 2,091,536 - +1.0 2,091,599 + CCNA_01949 0.21 +0.3 2,091,607 - CCNA_01949 0.23 -0.4 2,091,622 + CCNA_01949 0.26 +0.1 2,091,622 + CCNA_01949 0.26 +0.2 2,091,732 - CCNA_01949 0.49 +0.7 2,091,742 - CCNA_01949 0.51 +0.1 2,091,759 - CCNA_01949 0.54 +0.2 2,091,895 - CCNA_01949 0.82 -0.8 2,091,975 + +0.0 2,091,993 - +1.8 2,092,008 + +1.0 2,092,055 + -0.1 2,092,082 + -0.7 2,092,082 + +0.3 2,092,090 - +0.6
Or see this region's nucleotide sequence