Experiment: PYE with CHIR-090 4 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_01795 and CCNA_01797 are separated by 353 nucleotides CCNA_01797 and CCNA_01799 are separated by 165 nucleotides
CCNA_01795: CCNA_01795 - phosphopyruvate hydratase, at 1,921,855 to 1,923,135
_01795
CCNA_01797: CCNA_01797 - FtsB-related septum formation initiator, at 1,923,489 to 1,923,791
_01797
CCNA_01799: CCNA_01799 - pyruvate dehydrogenase E1 component alpha subunit, at 1,923,957 to 1,924,988
_01799
Position (kb)
1923
1924 Strain fitness (log2 ratio)
-2
-1
0
1 at 1922.542 kb on + strand, within CCNA_01795 at 1922.738 kb on + strand, within CCNA_01795 at 1922.808 kb on + strand, within CCNA_01795 at 1922.832 kb on + strand, within CCNA_01795 at 1922.835 kb on - strand, within CCNA_01795 at 1922.926 kb on + strand, within CCNA_01795 at 1922.934 kb on - strand, within CCNA_01795 at 1922.968 kb on - strand, within CCNA_01795 at 1922.985 kb on + strand, within CCNA_01795 at 1923.012 kb on + strand at 1923.049 kb on + strand at 1923.054 kb on + strand at 1923.108 kb on - strand at 1923.126 kb on - strand at 1923.145 kb on + strand at 1923.155 kb on + strand at 1923.190 kb on + strand at 1923.289 kb on + strand at 1923.312 kb on + strand at 1923.356 kb on - strand at 1923.406 kb on + strand at 1923.483 kb on + strand at 1923.758 kb on + strand, within CCNA_01797 at 1923.773 kb on + strand at 1923.773 kb on + strand at 1923.798 kb on - strand at 1923.821 kb on + strand at 1923.864 kb on + strand at 1924.395 kb on + strand, within CCNA_01799 at 1924.538 kb on + strand, within CCNA_01799
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with CHIR-090 4 ug/mL remove 1,922,542 + CCNA_01795 0.54 +0.6 1,922,738 + CCNA_01795 0.69 -0.4 1,922,808 + CCNA_01795 0.74 +0.2 1,922,832 + CCNA_01795 0.76 +0.0 1,922,835 - CCNA_01795 0.77 +0.0 1,922,926 + CCNA_01795 0.84 -2.0 1,922,934 - CCNA_01795 0.84 +0.6 1,922,968 - CCNA_01795 0.87 -0.2 1,922,985 + CCNA_01795 0.88 -1.0 1,923,012 + +0.3 1,923,049 + -0.8 1,923,054 + -1.7 1,923,108 - +0.0 1,923,126 - -0.2 1,923,145 + -0.1 1,923,155 + -1.2 1,923,190 + -0.2 1,923,289 + +0.3 1,923,312 + -0.3 1,923,356 - +1.1 1,923,406 + +0.7 1,923,483 + -0.4 1,923,758 + CCNA_01797 0.89 -0.6 1,923,773 + -1.0 1,923,773 + -1.2 1,923,798 - +0.0 1,923,821 + +0.8 1,923,864 + +0.6 1,924,395 + CCNA_01799 0.42 +0.6 1,924,538 + CCNA_01799 0.56 +0.5
Or see this region's nucleotide sequence