Experiment: PYE with CHIR-090 4 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_00255 and CCNA_00256 are separated by 18 nucleotides CCNA_00256 and CCNA_00257 are separated by 118 nucleotides CCNA_00257 and CCNA_00259 are separated by 342 nucleotides
CCNA_00255: CCNA_00255 - esterase, at 268,063 to 269,187
_00255
CCNA_00256: CCNA_00256 - pyridoxine kinase, at 269,206 to 270,057
_00256
CCNA_00257: CCNA_00257 - adenosylhomocysteinase, at 270,176 to 271,567
_00257
CCNA_00259: CCNA_00259 - hypothetical protein, at 271,910 to 272,620
_00259
Position (kb) 270
271
272 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 269.193 kb on + strand at 269.193 kb on + strand at 269.196 kb on - strand at 269.202 kb on - strand at 269.204 kb on + strand at 269.212 kb on - strand at 269.269 kb on + strand at 269.320 kb on - strand, within CCNA_00256 at 269.356 kb on + strand, within CCNA_00256 at 269.356 kb on + strand, within CCNA_00256 at 269.364 kb on - strand, within CCNA_00256 at 269.364 kb on - strand, within CCNA_00256 at 269.364 kb on - strand, within CCNA_00256 at 269.364 kb on - strand, within CCNA_00256 at 269.364 kb on - strand, within CCNA_00256 at 269.364 kb on - strand, within CCNA_00256 at 269.423 kb on - strand, within CCNA_00256 at 269.425 kb on + strand, within CCNA_00256 at 269.429 kb on + strand, within CCNA_00256 at 269.429 kb on + strand, within CCNA_00256 at 269.429 kb on + strand, within CCNA_00256 at 269.433 kb on - strand, within CCNA_00256 at 269.475 kb on - strand, within CCNA_00256 at 269.490 kb on - strand, within CCNA_00256 at 269.490 kb on - strand, within CCNA_00256 at 269.494 kb on + strand, within CCNA_00256 at 269.494 kb on + strand, within CCNA_00256 at 269.499 kb on + strand, within CCNA_00256 at 269.501 kb on + strand, within CCNA_00256 at 269.504 kb on + strand, within CCNA_00256 at 269.504 kb on + strand, within CCNA_00256 at 269.504 kb on + strand, within CCNA_00256 at 269.507 kb on - strand, within CCNA_00256 at 269.507 kb on - strand, within CCNA_00256 at 269.507 kb on - strand, within CCNA_00256 at 269.512 kb on - strand, within CCNA_00256 at 269.519 kb on + strand, within CCNA_00256 at 269.600 kb on - strand, within CCNA_00256 at 269.602 kb on - strand, within CCNA_00256 at 269.602 kb on - strand, within CCNA_00256 at 269.634 kb on + strand, within CCNA_00256 at 269.644 kb on + strand, within CCNA_00256 at 269.679 kb on - strand, within CCNA_00256 at 269.679 kb on - strand, within CCNA_00256 at 269.680 kb on - strand, within CCNA_00256 at 269.725 kb on + strand, within CCNA_00256 at 269.733 kb on - strand, within CCNA_00256 at 269.733 kb on - strand, within CCNA_00256 at 269.733 kb on - strand, within CCNA_00256 at 269.765 kb on + strand, within CCNA_00256 at 269.773 kb on - strand, within CCNA_00256 at 269.799 kb on - strand, within CCNA_00256 at 269.801 kb on - strand, within CCNA_00256 at 269.827 kb on - strand, within CCNA_00256 at 269.876 kb on + strand, within CCNA_00256 at 269.884 kb on - strand, within CCNA_00256 at 269.969 kb on + strand, within CCNA_00256 at 269.969 kb on + strand, within CCNA_00256 at 269.969 kb on + strand, within CCNA_00256 at 269.969 kb on + strand, within CCNA_00256 at 269.969 kb on + strand, within CCNA_00256 at 269.977 kb on - strand at 269.977 kb on - strand at 269.977 kb on - strand at 269.977 kb on - strand at 269.977 kb on - strand at 269.987 kb on + strand at 269.987 kb on + strand at 269.992 kb on + strand at 269.992 kb on + strand at 269.992 kb on + strand at 269.995 kb on - strand at 270.000 kb on - strand at 270.030 kb on + strand at 270.040 kb on + strand at 270.048 kb on - strand at 270.048 kb on - strand at 270.082 kb on - strand at 270.084 kb on + strand at 270.084 kb on + strand at 270.084 kb on + strand at 270.084 kb on + strand at 270.084 kb on + strand at 270.084 kb on + strand at 270.084 kb on + strand at 270.084 kb on + strand at 270.084 kb on + strand at 270.092 kb on - strand at 270.092 kb on - strand at 270.092 kb on - strand at 270.092 kb on - strand at 270.092 kb on - strand at 270.092 kb on - strand at 270.094 kb on - strand at 270.097 kb on - strand at 270.162 kb on + strand at 270.182 kb on - strand at 270.198 kb on - strand at 270.203 kb on - strand at 270.219 kb on - strand at 270.240 kb on + strand at 270.248 kb on - strand at 270.328 kb on - strand, within CCNA_00257 at 270.329 kb on - strand, within CCNA_00257 at 270.378 kb on - strand, within CCNA_00257 at 270.390 kb on - strand, within CCNA_00257 at 270.533 kb on + strand, within CCNA_00257 at 270.583 kb on - strand, within CCNA_00257 at 270.636 kb on - strand, within CCNA_00257 at 270.695 kb on - strand, within CCNA_00257 at 270.731 kb on - strand, within CCNA_00257 at 270.732 kb on + strand, within CCNA_00257 at 270.736 kb on - strand, within CCNA_00257 at 270.773 kb on - strand, within CCNA_00257 at 270.817 kb on - strand, within CCNA_00257 at 270.846 kb on - strand, within CCNA_00257 at 270.864 kb on + strand, within CCNA_00257 at 270.888 kb on + strand, within CCNA_00257 at 270.896 kb on - strand, within CCNA_00257 at 270.896 kb on - strand, within CCNA_00257 at 270.903 kb on + strand, within CCNA_00257 at 270.973 kb on + strand, within CCNA_00257 at 270.977 kb on - strand, within CCNA_00257 at 270.991 kb on - strand, within CCNA_00257 at 271.011 kb on - strand, within CCNA_00257 at 271.024 kb on - strand, within CCNA_00257 at 271.115 kb on + strand, within CCNA_00257 at 271.123 kb on - strand, within CCNA_00257 at 271.123 kb on - strand, within CCNA_00257 at 271.160 kb on + strand, within CCNA_00257 at 271.168 kb on - strand, within CCNA_00257 at 271.168 kb on - strand, within CCNA_00257 at 271.173 kb on - strand, within CCNA_00257 at 271.200 kb on + strand, within CCNA_00257 at 271.247 kb on + strand, within CCNA_00257 at 271.288 kb on - strand, within CCNA_00257 at 271.319 kb on + strand, within CCNA_00257 at 271.322 kb on - strand, within CCNA_00257 at 271.322 kb on - strand, within CCNA_00257 at 271.327 kb on - strand, within CCNA_00257 at 271.327 kb on - strand, within CCNA_00257 at 271.390 kb on + strand, within CCNA_00257 at 271.407 kb on + strand, within CCNA_00257 at 271.586 kb on + strand at 271.610 kb on - strand at 271.650 kb on + strand at 271.689 kb on + strand at 271.718 kb on + strand at 271.722 kb on + strand at 271.738 kb on - strand at 271.741 kb on - strand at 271.741 kb on - strand at 271.759 kb on - strand at 271.761 kb on + strand at 271.766 kb on + strand at 271.769 kb on - strand at 271.769 kb on - strand at 271.769 kb on - strand at 271.769 kb on - strand at 271.774 kb on - strand at 271.809 kb on + strand at 271.809 kb on + strand at 271.819 kb on - strand at 271.824 kb on + strand at 271.834 kb on + strand at 271.839 kb on + strand at 271.839 kb on + strand at 271.839 kb on + strand at 271.839 kb on + strand at 271.842 kb on - strand at 271.842 kb on - strand at 271.844 kb on + strand at 271.844 kb on + strand at 271.844 kb on + strand at 271.844 kb on + strand at 271.847 kb on - strand at 271.847 kb on - strand at 271.847 kb on - strand at 271.852 kb on - strand at 271.852 kb on - strand at 271.852 kb on - strand at 271.852 kb on - strand at 271.852 kb on - strand at 271.852 kb on - strand at 271.852 kb on - strand at 271.869 kb on + strand at 271.877 kb on - strand at 271.891 kb on + strand at 271.899 kb on - strand at 271.912 kb on + strand at 271.919 kb on + strand at 271.919 kb on + strand at 271.924 kb on + strand at 271.951 kb on - strand at 271.970 kb on + strand at 271.978 kb on - strand at 271.991 kb on + strand, within CCNA_00259 at 271.991 kb on + strand, within CCNA_00259 at 272.005 kb on + strand, within CCNA_00259 at 272.029 kb on + strand, within CCNA_00259 at 272.037 kb on - strand, within CCNA_00259 at 272.049 kb on + strand, within CCNA_00259 at 272.049 kb on + strand, within CCNA_00259 at 272.049 kb on + strand, within CCNA_00259 at 272.049 kb on + strand, within CCNA_00259 at 272.057 kb on - strand, within CCNA_00259 at 272.057 kb on - strand, within CCNA_00259 at 272.093 kb on - strand, within CCNA_00259 at 272.110 kb on + strand, within CCNA_00259 at 272.110 kb on + strand, within CCNA_00259 at 272.110 kb on + strand, within CCNA_00259 at 272.117 kb on + strand, within CCNA_00259 at 272.117 kb on + strand, within CCNA_00259 at 272.117 kb on + strand, within CCNA_00259 at 272.118 kb on - strand, within CCNA_00259 at 272.125 kb on - strand, within CCNA_00259 at 272.125 kb on - strand, within CCNA_00259 at 272.130 kb on - strand, within CCNA_00259 at 272.137 kb on - strand, within CCNA_00259 at 272.188 kb on + strand, within CCNA_00259 at 272.188 kb on + strand, within CCNA_00259 at 272.188 kb on + strand, within CCNA_00259 at 272.191 kb on - strand, within CCNA_00259 at 272.196 kb on - strand, within CCNA_00259 at 272.196 kb on - strand, within CCNA_00259 at 272.218 kb on + strand, within CCNA_00259 at 272.265 kb on + strand, within CCNA_00259 at 272.266 kb on - strand, within CCNA_00259 at 272.276 kb on + strand, within CCNA_00259 at 272.315 kb on + strand, within CCNA_00259 at 272.342 kb on + strand, within CCNA_00259 at 272.347 kb on + strand, within CCNA_00259 at 272.399 kb on + strand, within CCNA_00259 at 272.408 kb on + strand, within CCNA_00259 at 272.420 kb on + strand, within CCNA_00259 at 272.429 kb on + strand, within CCNA_00259 at 272.459 kb on - strand, within CCNA_00259 at 272.492 kb on + strand, within CCNA_00259 at 272.519 kb on - strand, within CCNA_00259 at 272.519 kb on - strand, within CCNA_00259 at 272.522 kb on + strand, within CCNA_00259 at 272.522 kb on + strand, within CCNA_00259 at 272.522 kb on + strand, within CCNA_00259 at 272.534 kb on + strand, within CCNA_00259 at 272.543 kb on + strand, within CCNA_00259
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with CHIR-090 4 ug/mL remove 269,193 + -0.1 269,193 + -0.2 269,196 - +0.3 269,202 - -0.5 269,204 + -0.9 269,212 - +0.3 269,269 + -0.6 269,320 - CCNA_00256 0.13 +1.3 269,356 + CCNA_00256 0.18 -0.0 269,356 + CCNA_00256 0.18 -0.6 269,364 - CCNA_00256 0.19 -0.5 269,364 - CCNA_00256 0.19 +0.1 269,364 - CCNA_00256 0.19 +0.2 269,364 - CCNA_00256 0.19 +0.6 269,364 - CCNA_00256 0.19 +0.6 269,364 - CCNA_00256 0.19 -0.7 269,423 - CCNA_00256 0.25 +0.8 269,425 + CCNA_00256 0.26 -0.3 269,429 + CCNA_00256 0.26 -0.6 269,429 + CCNA_00256 0.26 +0.8 269,429 + CCNA_00256 0.26 -0.2 269,433 - CCNA_00256 0.27 +0.0 269,475 - CCNA_00256 0.32 +0.7 269,490 - CCNA_00256 0.33 +0.4 269,490 - CCNA_00256 0.33 +0.5 269,494 + CCNA_00256 0.34 +0.5 269,494 + CCNA_00256 0.34 -1.5 269,499 + CCNA_00256 0.34 -0.6 269,501 + CCNA_00256 0.35 -0.5 269,504 + CCNA_00256 0.35 -0.1 269,504 + CCNA_00256 0.35 -0.5 269,504 + CCNA_00256 0.35 -0.4 269,507 - CCNA_00256 0.35 +0.3 269,507 - CCNA_00256 0.35 -0.2 269,507 - CCNA_00256 0.35 +2.3 269,512 - CCNA_00256 0.36 +0.5 269,519 + CCNA_00256 0.37 -0.4 269,600 - CCNA_00256 0.46 +1.3 269,602 - CCNA_00256 0.46 -0.7 269,602 - CCNA_00256 0.46 +0.1 269,634 + CCNA_00256 0.50 +0.3 269,644 + CCNA_00256 0.51 -0.2 269,679 - CCNA_00256 0.56 -0.0 269,679 - CCNA_00256 0.56 -0.4 269,680 - CCNA_00256 0.56 +0.1 269,725 + CCNA_00256 0.61 +0.8 269,733 - CCNA_00256 0.62 -0.8 269,733 - CCNA_00256 0.62 +0.7 269,733 - CCNA_00256 0.62 -0.1 269,765 + CCNA_00256 0.66 -0.4 269,773 - CCNA_00256 0.67 +0.1 269,799 - CCNA_00256 0.70 +0.1 269,801 - CCNA_00256 0.70 -0.4 269,827 - CCNA_00256 0.73 +0.0 269,876 + CCNA_00256 0.79 -0.2 269,884 - CCNA_00256 0.80 -0.8 269,969 + CCNA_00256 0.90 -0.3 269,969 + CCNA_00256 0.90 -0.4 269,969 + CCNA_00256 0.90 +0.1 269,969 + CCNA_00256 0.90 +0.6 269,969 + CCNA_00256 0.90 -0.4 269,977 - +0.3 269,977 - +0.2 269,977 - -0.1 269,977 - +0.0 269,977 - +0.1 269,987 + -0.2 269,987 + +0.8 269,992 + -0.6 269,992 + -0.4 269,992 + +0.4 269,995 - +0.1 270,000 - +0.5 270,030 + -0.3 270,040 + -0.2 270,048 - +0.0 270,048 - -0.3 270,082 - +0.4 270,084 + -0.6 270,084 + -1.1 270,084 + +1.3 270,084 + +0.0 270,084 + +0.2 270,084 + +0.5 270,084 + +0.9 270,084 + +0.0 270,084 + -0.4 270,092 - -1.2 270,092 - -1.0 270,092 - +0.5 270,092 - -0.5 270,092 - +0.4 270,092 - +0.3 270,094 - +0.4 270,097 - -0.3 270,162 + +0.1 270,182 - +0.3 270,198 - -1.2 270,203 - -0.5 270,219 - -0.5 270,240 + -0.6 270,248 - -0.8 270,328 - CCNA_00257 0.11 +0.1 270,329 - CCNA_00257 0.11 -1.4 270,378 - CCNA_00257 0.15 -0.1 270,390 - CCNA_00257 0.15 -1.2 270,533 + CCNA_00257 0.26 -0.7 270,583 - CCNA_00257 0.29 -1.1 270,636 - CCNA_00257 0.33 -1.0 270,695 - CCNA_00257 0.37 -0.8 270,731 - CCNA_00257 0.40 -0.2 270,732 + CCNA_00257 0.40 +0.3 270,736 - CCNA_00257 0.40 -0.2 270,773 - CCNA_00257 0.43 -1.6 270,817 - CCNA_00257 0.46 +0.7 270,846 - CCNA_00257 0.48 -0.9 270,864 + CCNA_00257 0.49 -0.5 270,888 + CCNA_00257 0.51 -0.7 270,896 - CCNA_00257 0.52 -1.8 270,896 - CCNA_00257 0.52 -0.9 270,903 + CCNA_00257 0.52 -1.8 270,973 + CCNA_00257 0.57 +0.3 270,977 - CCNA_00257 0.58 +1.3 270,991 - CCNA_00257 0.59 +0.2 271,011 - CCNA_00257 0.60 +0.6 271,024 - CCNA_00257 0.61 -1.1 271,115 + CCNA_00257 0.67 -1.8 271,123 - CCNA_00257 0.68 -0.4 271,123 - CCNA_00257 0.68 -0.7 271,160 + CCNA_00257 0.71 -0.3 271,168 - CCNA_00257 0.71 -0.3 271,168 - CCNA_00257 0.71 -1.9 271,173 - CCNA_00257 0.72 -1.0 271,200 + CCNA_00257 0.74 -1.5 271,247 + CCNA_00257 0.77 -0.3 271,288 - CCNA_00257 0.80 -1.0 271,319 + CCNA_00257 0.82 -1.4 271,322 - CCNA_00257 0.82 -0.7 271,322 - CCNA_00257 0.82 -0.4 271,327 - CCNA_00257 0.83 -1.0 271,327 - CCNA_00257 0.83 -0.3 271,390 + CCNA_00257 0.87 -2.5 271,407 + CCNA_00257 0.88 -0.8 271,586 + -0.6 271,610 - +0.0 271,650 + +0.8 271,689 + +0.6 271,718 + +0.0 271,722 + -0.6 271,738 - -0.3 271,741 - -1.4 271,741 - -0.1 271,759 - -1.0 271,761 + +0.5 271,766 + -0.4 271,769 - -0.2 271,769 - +0.6 271,769 - +0.7 271,769 - +0.2 271,774 - +0.0 271,809 + +0.5 271,809 + -0.9 271,819 - +0.3 271,824 + -0.3 271,834 + +0.2 271,839 + -0.1 271,839 + +0.6 271,839 + -0.5 271,839 + -0.3 271,842 - -0.5 271,842 - +0.7 271,844 + -0.1 271,844 + -0.3 271,844 + +0.4 271,844 + -0.5 271,847 - -0.2 271,847 - +0.6 271,847 - +0.8 271,852 - +0.3 271,852 - +0.4 271,852 - +0.9 271,852 - -1.1 271,852 - +0.4 271,852 - -0.9 271,852 - -1.1 271,869 + -0.3 271,877 - -0.1 271,891 + -0.3 271,899 - -0.7 271,912 + +0.9 271,919 + -2.5 271,919 + -1.7 271,924 + +0.1 271,951 - -0.1 271,970 + -1.7 271,978 - +0.6 271,991 + CCNA_00259 0.11 -0.5 271,991 + CCNA_00259 0.11 +0.6 272,005 + CCNA_00259 0.13 +0.4 272,029 + CCNA_00259 0.17 +0.2 272,037 - CCNA_00259 0.18 +0.7 272,049 + CCNA_00259 0.20 -0.3 272,049 + CCNA_00259 0.20 +2.3 272,049 + CCNA_00259 0.20 -0.4 272,049 + CCNA_00259 0.20 -1.2 272,057 - CCNA_00259 0.21 +0.0 272,057 - CCNA_00259 0.21 +0.8 272,093 - CCNA_00259 0.26 +0.6 272,110 + CCNA_00259 0.28 -0.6 272,110 + CCNA_00259 0.28 -0.6 272,110 + CCNA_00259 0.28 -0.6 272,117 + CCNA_00259 0.29 +2.0 272,117 + CCNA_00259 0.29 -0.0 272,117 + CCNA_00259 0.29 -0.4 272,118 - CCNA_00259 0.29 -0.6 272,125 - CCNA_00259 0.30 -0.4 272,125 - CCNA_00259 0.30 +0.9 272,130 - CCNA_00259 0.31 +0.5 272,137 - CCNA_00259 0.32 -1.7 272,188 + CCNA_00259 0.39 -0.3 272,188 + CCNA_00259 0.39 -2.8 272,188 + CCNA_00259 0.39 -1.0 272,191 - CCNA_00259 0.40 -0.1 272,196 - CCNA_00259 0.40 -0.0 272,196 - CCNA_00259 0.40 -0.9 272,218 + CCNA_00259 0.43 +0.1 272,265 + CCNA_00259 0.50 -0.9 272,266 - CCNA_00259 0.50 +0.1 272,276 + CCNA_00259 0.51 +0.1 272,315 + CCNA_00259 0.57 -0.6 272,342 + CCNA_00259 0.61 -0.0 272,347 + CCNA_00259 0.61 -0.1 272,399 + CCNA_00259 0.69 -2.1 272,408 + CCNA_00259 0.70 -0.6 272,420 + CCNA_00259 0.72 -0.6 272,429 + CCNA_00259 0.73 +0.7 272,459 - CCNA_00259 0.77 +0.1 272,492 + CCNA_00259 0.82 -0.5 272,519 - CCNA_00259 0.86 +1.0 272,519 - CCNA_00259 0.86 -0.0 272,522 + CCNA_00259 0.86 -0.6 272,522 + CCNA_00259 0.86 -0.1 272,522 + CCNA_00259 0.86 +0.2 272,534 + CCNA_00259 0.88 -0.3 272,543 + CCNA_00259 0.89 +0.3
Or see this region's nucleotide sequence