Experiment: PYE with CHIR-090 4 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_00048 and CCNA_00049 are separated by 119 nucleotides CCNA_00049 and CCNA_00050 are separated by 27 nucleotides CCNA_00050 and CCNA_00051 overlap by 4 nucleotides CCNA_00051 and CCNA_00052 are separated by 8 nucleotides
CCNA_00048: CCNA_00048 - S-adenosylmethionine synthetase, at 50,902 to 52,125
_00048
CCNA_00049: CCNA_00049 - HTH transcriptional regulator, at 52,245 to 52,679
_00049
CCNA_00050: CCNA_00050 - apolipoprotein N-acyltransferase, at 52,707 to 54,299
_00050
CCNA_00051: CCNA_00051 - magnesium and cobalt efflux protein CorC, at 54,296 to 55,222
_00051
CCNA_00052: CCNA_00052 - metal-dependent hydrolase/metalloproteinase, at 55,231 to 55,686
_00052
Position (kb) 52
53
54
55 Strain fitness (log2 ratio) -2
-1
0
1
2 at 52.230 kb on - strand at 52.251 kb on - strand at 52.258 kb on - strand at 52.324 kb on + strand, within CCNA_00049 at 52.331 kb on + strand, within CCNA_00049 at 52.331 kb on + strand, within CCNA_00049 at 52.331 kb on + strand, within CCNA_00049 at 52.393 kb on + strand, within CCNA_00049 at 52.393 kb on + strand, within CCNA_00049 at 52.430 kb on + strand, within CCNA_00049 at 52.430 kb on + strand, within CCNA_00049 at 52.430 kb on + strand, within CCNA_00049 at 52.438 kb on - strand, within CCNA_00049 at 52.469 kb on + strand, within CCNA_00049 at 52.554 kb on - strand, within CCNA_00049 at 52.577 kb on + strand, within CCNA_00049 at 52.636 kb on + strand at 52.636 kb on + strand at 52.644 kb on - strand at 52.753 kb on + strand at 52.775 kb on + strand at 53.088 kb on - strand, within CCNA_00050 at 53.637 kb on - strand, within CCNA_00050 at 53.958 kb on - strand, within CCNA_00050 at 54.262 kb on + strand at 54.262 kb on + strand at 54.262 kb on + strand at 54.262 kb on + strand at 54.270 kb on - strand at 54.309 kb on + strand at 54.329 kb on - strand at 54.479 kb on - strand, within CCNA_00051 at 54.502 kb on - strand, within CCNA_00051 at 54.534 kb on - strand, within CCNA_00051 at 54.534 kb on - strand, within CCNA_00051 at 54.541 kb on - strand, within CCNA_00051 at 54.551 kb on - strand, within CCNA_00051 at 54.551 kb on - strand, within CCNA_00051 at 54.561 kb on - strand, within CCNA_00051 at 54.564 kb on + strand, within CCNA_00051 at 54.631 kb on - strand, within CCNA_00051 at 54.634 kb on - strand, within CCNA_00051 at 54.667 kb on + strand, within CCNA_00051 at 54.721 kb on - strand, within CCNA_00051 at 54.757 kb on - strand, within CCNA_00051 at 54.757 kb on - strand, within CCNA_00051 at 54.774 kb on - strand, within CCNA_00051 at 54.784 kb on + strand, within CCNA_00051 at 54.793 kb on - strand, within CCNA_00051 at 54.795 kb on + strand, within CCNA_00051 at 54.814 kb on + strand, within CCNA_00051 at 54.870 kb on + strand, within CCNA_00051 at 54.878 kb on - strand, within CCNA_00051 at 54.907 kb on - strand, within CCNA_00051 at 55.011 kb on + strand, within CCNA_00051 at 55.084 kb on - strand, within CCNA_00051 at 55.196 kb on + strand at 55.208 kb on - strand at 55.219 kb on + strand at 55.224 kb on + strand at 55.224 kb on + strand at 55.224 kb on + strand at 55.224 kb on + strand at 55.224 kb on + strand at 55.232 kb on - strand at 55.232 kb on - strand at 55.232 kb on - strand at 55.232 kb on - strand at 55.232 kb on - strand at 55.260 kb on - strand at 55.261 kb on - strand at 55.270 kb on + strand at 55.278 kb on - strand, within CCNA_00052
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with CHIR-090 4 ug/mL remove 52,230 - +1.1 52,251 - -0.5 52,258 - +0.9 52,324 + CCNA_00049 0.18 -1.6 52,331 + CCNA_00049 0.20 +0.6 52,331 + CCNA_00049 0.20 -0.8 52,331 + CCNA_00049 0.20 -0.2 52,393 + CCNA_00049 0.34 +0.3 52,393 + CCNA_00049 0.34 +1.6 52,430 + CCNA_00049 0.43 -0.9 52,430 + CCNA_00049 0.43 -1.0 52,430 + CCNA_00049 0.43 +0.6 52,438 - CCNA_00049 0.44 +0.5 52,469 + CCNA_00049 0.51 +0.3 52,554 - CCNA_00049 0.71 -0.9 52,577 + CCNA_00049 0.76 +1.0 52,636 + -0.1 52,636 + -1.0 52,644 - +0.5 52,753 + -1.1 52,775 + +1.1 53,088 - CCNA_00050 0.24 -0.0 53,637 - CCNA_00050 0.58 -0.9 53,958 - CCNA_00050 0.79 -0.7 54,262 + -0.5 54,262 + +0.1 54,262 + -0.3 54,262 + +0.2 54,270 - +0.0 54,309 + +0.5 54,329 - -1.1 54,479 - CCNA_00051 0.20 +0.4 54,502 - CCNA_00051 0.22 +1.3 54,534 - CCNA_00051 0.26 +0.5 54,534 - CCNA_00051 0.26 +0.1 54,541 - CCNA_00051 0.26 -0.8 54,551 - CCNA_00051 0.28 +0.4 54,551 - CCNA_00051 0.28 +0.9 54,561 - CCNA_00051 0.29 -0.1 54,564 + CCNA_00051 0.29 -0.2 54,631 - CCNA_00051 0.36 -0.5 54,634 - CCNA_00051 0.36 +1.3 54,667 + CCNA_00051 0.40 +0.1 54,721 - CCNA_00051 0.46 -0.8 54,757 - CCNA_00051 0.50 +0.3 54,757 - CCNA_00051 0.50 +0.7 54,774 - CCNA_00051 0.52 -0.3 54,784 + CCNA_00051 0.53 +0.1 54,793 - CCNA_00051 0.54 -0.0 54,795 + CCNA_00051 0.54 +0.6 54,814 + CCNA_00051 0.56 +1.1 54,870 + CCNA_00051 0.62 +1.2 54,878 - CCNA_00051 0.63 +0.3 54,907 - CCNA_00051 0.66 -0.2 55,011 + CCNA_00051 0.77 +0.4 55,084 - CCNA_00051 0.85 +0.5 55,196 + +0.1 55,208 - +1.9 55,219 + +0.8 55,224 + -1.9 55,224 + +1.7 55,224 + +2.3 55,224 + +0.7 55,224 + +1.0 55,232 - +1.3 55,232 - +0.9 55,232 - -0.6 55,232 - -0.3 55,232 - -1.2 55,260 - -0.3 55,261 - +1.1 55,270 + +0.0 55,278 - CCNA_00052 0.10 +0.8
Or see this region's nucleotide sequence