Experiment: MS_media with m-arsenite 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_3700 and EX28DRAFT_3701 are separated by 10 nucleotides EX28DRAFT_3701 and EX28DRAFT_3702 are separated by 174 nucleotides EX28DRAFT_3702 and EX28DRAFT_3703 are separated by 87 nucleotides EX28DRAFT_3703 and EX28DRAFT_3704 are separated by 9 nucleotides
EX28DRAFT_3700: EX28DRAFT_3700 - Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB, at 370,509 to 371,891
_3700
EX28DRAFT_3701: EX28DRAFT_3701 - prepilin-type N-terminal cleavage/methylation domain, at 371,902 to 372,339
_3701
EX28DRAFT_3702: EX28DRAFT_3702 - nicotinate-nucleotide pyrophosphorylase [carboxylating] (EC 2.4.2.19), at 372,514 to 373,407
_3702
EX28DRAFT_3703: EX28DRAFT_3703 - N-acetylmuramoyl-L-alanine amidase, at 373,495 to 374,046
_3703
EX28DRAFT_3704: EX28DRAFT_3704 - Membrane protein required for beta-lactamase induction, at 374,056 to 374,910
_3704
Position (kb) 372
373
374 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 371.635 kb on + strand, within EX28DRAFT_3700 at 371.645 kb on + strand, within EX28DRAFT_3700 at 371.698 kb on - strand, within EX28DRAFT_3700 at 371.965 kb on - strand, within EX28DRAFT_3701 at 372.124 kb on - strand, within EX28DRAFT_3701 at 372.217 kb on - strand, within EX28DRAFT_3701 at 372.339 kb on + strand at 372.340 kb on - strand at 372.340 kb on - strand at 372.350 kb on + strand at 372.578 kb on + strand at 372.578 kb on + strand at 372.578 kb on + strand at 372.578 kb on + strand at 372.579 kb on - strand at 372.579 kb on - strand at 372.579 kb on - strand at 372.766 kb on + strand, within EX28DRAFT_3702 at 372.838 kb on - strand, within EX28DRAFT_3702 at 372.838 kb on - strand, within EX28DRAFT_3702 at 372.838 kb on - strand, within EX28DRAFT_3702 at 372.915 kb on - strand, within EX28DRAFT_3702 at 372.915 kb on - strand, within EX28DRAFT_3702 at 372.915 kb on - strand, within EX28DRAFT_3702 at 373.347 kb on + strand at 373.350 kb on + strand at 373.350 kb on + strand at 373.350 kb on + strand at 373.350 kb on + strand at 373.350 kb on + strand at 373.351 kb on - strand at 373.351 kb on - strand at 373.351 kb on - strand at 373.351 kb on - strand at 373.351 kb on - strand at 373.351 kb on - strand at 373.354 kb on - strand at 373.391 kb on + strand at 373.410 kb on + strand at 373.411 kb on - strand at 373.411 kb on - strand at 373.437 kb on - strand at 373.452 kb on + strand at 374.045 kb on - strand at 374.052 kb on + strand at 374.052 kb on + strand at 374.052 kb on + strand at 374.068 kb on - strand at 374.214 kb on + strand, within EX28DRAFT_3704 at 374.214 kb on + strand, within EX28DRAFT_3704 at 374.214 kb on + strand, within EX28DRAFT_3704 at 374.215 kb on - strand, within EX28DRAFT_3704 at 374.239 kb on + strand, within EX28DRAFT_3704 at 374.335 kb on + strand, within EX28DRAFT_3704 at 374.336 kb on - strand, within EX28DRAFT_3704
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media with m-arsenite 1 mM remove 371,635 + EX28DRAFT_3700 0.81 +1.1 371,645 + EX28DRAFT_3700 0.82 +0.4 371,698 - EX28DRAFT_3700 0.86 +0.2 371,965 - EX28DRAFT_3701 0.14 +0.0 372,124 - EX28DRAFT_3701 0.51 +0.6 372,217 - EX28DRAFT_3701 0.72 +0.7 372,339 + +0.2 372,340 - -0.8 372,340 - -3.4 372,350 + +0.1 372,578 + +1.1 372,578 + -0.7 372,578 + -2.3 372,578 + +0.9 372,579 - -0.2 372,579 - -0.8 372,579 - +2.6 372,766 + EX28DRAFT_3702 0.28 +0.6 372,838 - EX28DRAFT_3702 0.36 +1.3 372,838 - EX28DRAFT_3702 0.36 -0.1 372,838 - EX28DRAFT_3702 0.36 +0.8 372,915 - EX28DRAFT_3702 0.45 -2.0 372,915 - EX28DRAFT_3702 0.45 -3.1 372,915 - EX28DRAFT_3702 0.45 +1.0 373,347 + +0.1 373,350 + -0.1 373,350 + -2.1 373,350 + -0.9 373,350 + -0.2 373,350 + -4.4 373,351 - +1.2 373,351 - -1.1 373,351 - +0.1 373,351 - +0.7 373,351 - -1.7 373,351 - +0.5 373,354 - -0.2 373,391 + -1.6 373,410 + +0.2 373,411 - -0.6 373,411 - -0.7 373,437 - -0.1 373,452 + +0.2 374,045 - +0.2 374,052 + -1.2 374,052 + -1.5 374,052 + +1.0 374,068 - +0.5 374,214 + EX28DRAFT_3704 0.18 +0.5 374,214 + EX28DRAFT_3704 0.18 -1.3 374,214 + EX28DRAFT_3704 0.18 +0.8 374,215 - EX28DRAFT_3704 0.19 +1.1 374,239 + EX28DRAFT_3704 0.21 +0.8 374,335 + EX28DRAFT_3704 0.33 +0.0 374,336 - EX28DRAFT_3704 0.33 +0.5
Or see this region's nucleotide sequence