Experiment: MS_media with m-arsenite 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_3616 and EX28DRAFT_3617 are separated by 257 nucleotides EX28DRAFT_3617 and EX28DRAFT_3618 are separated by 32 nucleotides EX28DRAFT_3618 and EX28DRAFT_3619 are separated by 66 nucleotides
EX28DRAFT_3616: EX28DRAFT_3616 - threonine synthase, at 275,764 to 277,050
_3616
EX28DRAFT_3617: EX28DRAFT_3617 - Protein of unknown function (DUF2502), at 277,308 to 277,610
_3617
EX28DRAFT_3618: EX28DRAFT_3618 - Uncharacterized protein conserved in bacteria, at 277,643 to 278,416
_3618
EX28DRAFT_3619: EX28DRAFT_3619 - amino acid carrier protein, at 278,483 to 279,913
_3619
Position (kb) 277
278
279 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 276.807 kb on + strand, within EX28DRAFT_3616 at 276.808 kb on - strand, within EX28DRAFT_3616 at 276.808 kb on - strand, within EX28DRAFT_3616 at 276.920 kb on + strand, within EX28DRAFT_3616 at 277.087 kb on + strand at 277.112 kb on + strand at 277.200 kb on - strand at 277.267 kb on + strand at 277.313 kb on + strand at 277.393 kb on + strand, within EX28DRAFT_3617 at 277.394 kb on - strand, within EX28DRAFT_3617 at 277.394 kb on - strand, within EX28DRAFT_3617 at 277.403 kb on - strand, within EX28DRAFT_3617 at 277.428 kb on - strand, within EX28DRAFT_3617 at 277.488 kb on + strand, within EX28DRAFT_3617 at 277.489 kb on - strand, within EX28DRAFT_3617 at 277.489 kb on - strand, within EX28DRAFT_3617 at 277.644 kb on + strand at 277.704 kb on + strand at 277.890 kb on + strand, within EX28DRAFT_3618 at 277.890 kb on + strand, within EX28DRAFT_3618 at 277.890 kb on + strand, within EX28DRAFT_3618 at 277.891 kb on - strand, within EX28DRAFT_3618 at 277.891 kb on - strand, within EX28DRAFT_3618 at 277.891 kb on - strand, within EX28DRAFT_3618 at 277.891 kb on - strand, within EX28DRAFT_3618 at 277.893 kb on + strand, within EX28DRAFT_3618 at 277.894 kb on - strand, within EX28DRAFT_3618 at 277.894 kb on - strand, within EX28DRAFT_3618 at 277.986 kb on + strand, within EX28DRAFT_3618 at 278.155 kb on - strand, within EX28DRAFT_3618 at 278.155 kb on - strand, within EX28DRAFT_3618 at 278.168 kb on - strand, within EX28DRAFT_3618 at 278.299 kb on + strand, within EX28DRAFT_3618 at 278.328 kb on + strand, within EX28DRAFT_3618 at 278.416 kb on + strand at 278.477 kb on + strand at 278.485 kb on - strand at 278.669 kb on - strand, within EX28DRAFT_3619 at 278.732 kb on + strand, within EX28DRAFT_3619 at 278.732 kb on + strand, within EX28DRAFT_3619 at 278.733 kb on - strand, within EX28DRAFT_3619 at 278.788 kb on + strand, within EX28DRAFT_3619 at 278.789 kb on - strand, within EX28DRAFT_3619 at 278.806 kb on - strand, within EX28DRAFT_3619 at 278.811 kb on + strand, within EX28DRAFT_3619 at 278.989 kb on + strand, within EX28DRAFT_3619 at 279.211 kb on + strand, within EX28DRAFT_3619
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media with m-arsenite 1 mM remove 276,807 + EX28DRAFT_3616 0.81 -1.1 276,808 - EX28DRAFT_3616 0.81 -1.7 276,808 - EX28DRAFT_3616 0.81 -0.9 276,920 + EX28DRAFT_3616 0.90 +0.3 277,087 + -3.3 277,112 + +0.3 277,200 - -2.0 277,267 + +0.3 277,313 + -0.4 277,393 + EX28DRAFT_3617 0.28 -0.6 277,394 - EX28DRAFT_3617 0.28 +0.3 277,394 - EX28DRAFT_3617 0.28 -0.4 277,403 - EX28DRAFT_3617 0.31 +0.5 277,428 - EX28DRAFT_3617 0.40 +0.3 277,488 + EX28DRAFT_3617 0.59 +0.1 277,489 - EX28DRAFT_3617 0.60 +0.6 277,489 - EX28DRAFT_3617 0.60 -1.7 277,644 + +0.3 277,704 + -4.1 277,890 + EX28DRAFT_3618 0.32 -0.7 277,890 + EX28DRAFT_3618 0.32 +0.6 277,890 + EX28DRAFT_3618 0.32 +0.4 277,891 - EX28DRAFT_3618 0.32 -1.9 277,891 - EX28DRAFT_3618 0.32 +0.7 277,891 - EX28DRAFT_3618 0.32 +0.2 277,891 - EX28DRAFT_3618 0.32 +0.1 277,893 + EX28DRAFT_3618 0.32 +0.9 277,894 - EX28DRAFT_3618 0.32 -0.7 277,894 - EX28DRAFT_3618 0.32 +0.2 277,986 + EX28DRAFT_3618 0.44 -2.1 278,155 - EX28DRAFT_3618 0.66 -0.3 278,155 - EX28DRAFT_3618 0.66 +0.5 278,168 - EX28DRAFT_3618 0.68 -0.7 278,299 + EX28DRAFT_3618 0.85 +0.2 278,328 + EX28DRAFT_3618 0.89 -1.0 278,416 + -0.6 278,477 + +1.3 278,485 - +0.5 278,669 - EX28DRAFT_3619 0.13 +0.0 278,732 + EX28DRAFT_3619 0.17 -0.2 278,732 + EX28DRAFT_3619 0.17 +0.1 278,733 - EX28DRAFT_3619 0.17 +0.4 278,788 + EX28DRAFT_3619 0.21 +0.8 278,789 - EX28DRAFT_3619 0.21 +0.6 278,806 - EX28DRAFT_3619 0.23 +0.1 278,811 + EX28DRAFT_3619 0.23 -0.3 278,989 + EX28DRAFT_3619 0.35 +0.1 279,211 + EX28DRAFT_3619 0.51 +0.4
Or see this region's nucleotide sequence