Experiment: MS_media with m-arsenite 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_3381 and EX28DRAFT_3382 are separated by 148 nucleotides EX28DRAFT_3382 and EX28DRAFT_3383 are separated by 158 nucleotides EX28DRAFT_3383 and EX28DRAFT_3384 are separated by 169 nucleotides EX28DRAFT_3384 and EX28DRAFT_3385 are separated by 341 nucleotides
EX28DRAFT_3381: EX28DRAFT_3381 - Protein of unknown function (DUF1471), at 52,428 to 52,703
_3381
EX28DRAFT_3382: EX28DRAFT_3382 - Protein of unknown function (DUF1471), at 52,852 to 53,160
_3382
EX28DRAFT_3383: EX28DRAFT_3383 - Alpha/beta hydrolase family, at 53,319 to 54,041
_3383
EX28DRAFT_3384: EX28DRAFT_3384 - Protein of unknown function (DUF1471), at 54,211 to 54,486
_3384
EX28DRAFT_3385: EX28DRAFT_3385 - ribosomal protein S6, at 54,828 to 55,223
_3385
Position (kb)
53
54
55 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 52.429 kb on + strand at 52.527 kb on + strand, within EX28DRAFT_3381 at 52.572 kb on - strand, within EX28DRAFT_3381 at 52.722 kb on + strand at 52.722 kb on + strand at 52.723 kb on - strand at 52.756 kb on + strand at 52.790 kb on - strand at 52.811 kb on - strand at 52.811 kb on - strand at 52.828 kb on + strand at 52.854 kb on - strand at 52.857 kb on + strand at 52.857 kb on + strand at 52.858 kb on - strand at 52.858 kb on - strand at 52.858 kb on - strand at 52.894 kb on + strand, within EX28DRAFT_3382 at 52.926 kb on - strand, within EX28DRAFT_3382 at 52.926 kb on - strand, within EX28DRAFT_3382 at 52.929 kb on - strand, within EX28DRAFT_3382 at 53.024 kb on - strand, within EX28DRAFT_3382 at 53.154 kb on + strand at 53.160 kb on + strand at 53.160 kb on + strand at 53.160 kb on + strand at 53.161 kb on - strand at 53.181 kb on + strand at 53.181 kb on + strand at 53.182 kb on - strand at 53.186 kb on + strand at 53.186 kb on + strand at 53.186 kb on + strand at 53.186 kb on + strand at 53.187 kb on - strand at 53.242 kb on - strand at 53.242 kb on - strand at 53.272 kb on - strand at 53.289 kb on + strand at 53.298 kb on + strand at 53.298 kb on + strand at 53.336 kb on - strand at 53.614 kb on - strand, within EX28DRAFT_3383 at 54.065 kb on - strand at 54.109 kb on - strand at 54.112 kb on - strand at 54.112 kb on - strand at 54.112 kb on - strand at 54.163 kb on + strand at 54.163 kb on + strand at 54.163 kb on + strand at 54.164 kb on - strand at 54.164 kb on - strand at 54.164 kb on - strand at 54.164 kb on - strand at 54.169 kb on + strand at 54.218 kb on + strand at 54.222 kb on + strand at 54.223 kb on - strand at 54.284 kb on + strand, within EX28DRAFT_3384 at 54.284 kb on + strand, within EX28DRAFT_3384 at 54.285 kb on - strand, within EX28DRAFT_3384 at 54.285 kb on - strand, within EX28DRAFT_3384 at 54.352 kb on - strand, within EX28DRAFT_3384 at 54.363 kb on - strand, within EX28DRAFT_3384 at 54.415 kb on + strand, within EX28DRAFT_3384 at 54.416 kb on - strand, within EX28DRAFT_3384 at 54.490 kb on - strand at 54.504 kb on + strand at 54.505 kb on - strand at 54.557 kb on - strand at 54.563 kb on + strand at 54.563 kb on + strand at 54.645 kb on - strand at 54.658 kb on - strand at 54.658 kb on - strand at 54.684 kb on + strand at 54.685 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media with m-arsenite 1 mM remove 52,429 + -1.7 52,527 + EX28DRAFT_3381 0.36 +0.5 52,572 - EX28DRAFT_3381 0.52 +2.3 52,722 + -0.2 52,722 + +0.3 52,723 - -0.5 52,756 + +1.1 52,790 - -3.1 52,811 - -0.3 52,811 - -0.3 52,828 + -1.3 52,854 - +0.3 52,857 + +0.1 52,857 + +0.8 52,858 - -0.4 52,858 - +0.4 52,858 - +1.3 52,894 + EX28DRAFT_3382 0.14 +0.6 52,926 - EX28DRAFT_3382 0.24 +1.2 52,926 - EX28DRAFT_3382 0.24 -0.7 52,929 - EX28DRAFT_3382 0.25 +0.0 53,024 - EX28DRAFT_3382 0.56 -0.5 53,154 + +1.1 53,160 + -0.1 53,160 + -0.1 53,160 + +0.4 53,161 - +0.1 53,181 + +0.3 53,181 + +0.8 53,182 - -1.1 53,186 + +0.7 53,186 + +0.2 53,186 + +0.1 53,186 + -3.6 53,187 - -1.4 53,242 - +0.2 53,242 - -0.1 53,272 - -1.8 53,289 + -0.1 53,298 + +1.1 53,298 + +1.0 53,336 - +0.1 53,614 - EX28DRAFT_3383 0.41 +0.8 54,065 - -0.7 54,109 - +0.1 54,112 - -3.6 54,112 - -0.1 54,112 - +0.0 54,163 + +1.4 54,163 + -0.0 54,163 + +0.3 54,164 - -0.4 54,164 - -0.7 54,164 - +0.5 54,164 - -0.1 54,169 + -0.2 54,218 + -0.4 54,222 + +0.4 54,223 - +1.1 54,284 + EX28DRAFT_3384 0.26 -0.2 54,284 + EX28DRAFT_3384 0.26 +1.3 54,285 - EX28DRAFT_3384 0.27 +0.2 54,285 - EX28DRAFT_3384 0.27 +2.2 54,352 - EX28DRAFT_3384 0.51 -1.5 54,363 - EX28DRAFT_3384 0.55 +0.2 54,415 + EX28DRAFT_3384 0.74 +0.1 54,416 - EX28DRAFT_3384 0.74 +1.0 54,490 - +0.9 54,504 + -1.6 54,505 - -0.6 54,557 - -1.0 54,563 + +0.1 54,563 + +0.3 54,645 - -1.8 54,658 - -0.3 54,658 - +0.3 54,684 + +1.2 54,685 - -0.7
Or see this region's nucleotide sequence