Experiment: MS_media with m-arsenite 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_3345 and EX28DRAFT_3346 are separated by 37 nucleotides EX28DRAFT_3346 and EX28DRAFT_3347 are separated by 189 nucleotides EX28DRAFT_3347 and EX28DRAFT_3348 are separated by 27 nucleotides EX28DRAFT_3348 and EX28DRAFT_3349 overlap by 4 nucleotides EX28DRAFT_3349 and EX28DRAFT_3350 are separated by 138 nucleotides
EX28DRAFT_3345: EX28DRAFT_3345 - Predicted small secreted protein, at 14,630 to 14,776
_3345
EX28DRAFT_3346: EX28DRAFT_3346 - Response regulator containing a CheY-like receiver domain and an HTH DNA-binding domain, at 14,814 to 15,413
_3346
EX28DRAFT_3347: EX28DRAFT_3347 - RNA, at 15,603 to 15,647
_3347
EX28DRAFT_3348: EX28DRAFT_3348 - Membrane transporters of cations and cationic drugs, at 15,675 to 15,992
_3348
EX28DRAFT_3349: EX28DRAFT_3349 - Bacterial lipocalin, at 15,989 to 16,519
_3349
EX28DRAFT_3350: EX28DRAFT_3350 - Beta-lactamase class C and other penicillin binding proteins, at 16,658 to 17,800
_3350
Position (kb)
15
16 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 14.695 kb on + strand, within EX28DRAFT_3345 at 14.698 kb on + strand, within EX28DRAFT_3345 at 14.761 kb on + strand, within EX28DRAFT_3345 at 14.774 kb on + strand at 14.775 kb on - strand at 14.778 kb on + strand at 14.783 kb on + strand at 14.784 kb on - strand at 14.784 kb on - strand at 14.784 kb on - strand at 14.785 kb on - strand at 14.893 kb on + strand, within EX28DRAFT_3346 at 14.893 kb on + strand, within EX28DRAFT_3346 at 14.893 kb on + strand, within EX28DRAFT_3346 at 14.894 kb on - strand, within EX28DRAFT_3346 at 14.897 kb on + strand, within EX28DRAFT_3346 at 14.897 kb on + strand, within EX28DRAFT_3346 at 14.897 kb on + strand, within EX28DRAFT_3346 at 14.898 kb on - strand, within EX28DRAFT_3346 at 14.898 kb on - strand, within EX28DRAFT_3346 at 14.898 kb on - strand, within EX28DRAFT_3346 at 14.898 kb on - strand, within EX28DRAFT_3346 at 14.899 kb on + strand, within EX28DRAFT_3346 at 14.900 kb on - strand, within EX28DRAFT_3346 at 14.900 kb on - strand, within EX28DRAFT_3346 at 15.222 kb on - strand, within EX28DRAFT_3346 at 15.322 kb on + strand, within EX28DRAFT_3346 at 15.322 kb on + strand, within EX28DRAFT_3346 at 15.413 kb on + strand at 15.471 kb on + strand at 15.471 kb on + strand at 15.492 kb on - strand at 15.492 kb on - strand at 15.514 kb on - strand at 15.523 kb on - strand at 15.534 kb on + strand at 15.584 kb on + strand at 15.654 kb on + strand at 15.654 kb on + strand at 15.655 kb on - strand at 15.657 kb on + strand at 15.657 kb on + strand at 15.658 kb on - strand at 15.658 kb on - strand at 15.695 kb on + strand at 15.719 kb on - strand, within EX28DRAFT_3348 at 15.775 kb on + strand, within EX28DRAFT_3348 at 15.775 kb on + strand, within EX28DRAFT_3348 at 15.800 kb on + strand, within EX28DRAFT_3348 at 15.800 kb on + strand, within EX28DRAFT_3348 at 15.800 kb on + strand, within EX28DRAFT_3348 at 15.801 kb on - strand, within EX28DRAFT_3348 at 15.812 kb on - strand, within EX28DRAFT_3348 at 15.849 kb on + strand, within EX28DRAFT_3348 at 16.081 kb on + strand, within EX28DRAFT_3349 at 16.173 kb on + strand, within EX28DRAFT_3349 at 16.283 kb on - strand, within EX28DRAFT_3349 at 16.329 kb on + strand, within EX28DRAFT_3349 at 16.329 kb on + strand, within EX28DRAFT_3349 at 16.349 kb on + strand, within EX28DRAFT_3349 at 16.350 kb on - strand, within EX28DRAFT_3349 at 16.389 kb on + strand, within EX28DRAFT_3349 at 16.390 kb on - strand, within EX28DRAFT_3349 at 16.463 kb on - strand, within EX28DRAFT_3349 at 16.519 kb on + strand at 16.519 kb on + strand at 16.520 kb on - strand at 16.545 kb on + strand at 16.552 kb on - strand at 16.656 kb on + strand at 16.656 kb on + strand at 16.660 kb on - strand at 16.660 kb on - strand at 16.768 kb on - strand at 16.768 kb on - strand at 16.768 kb on - strand at 16.768 kb on - strand at 16.798 kb on + strand, within EX28DRAFT_3350 at 16.874 kb on - strand, within EX28DRAFT_3350 at 16.919 kb on - strand, within EX28DRAFT_3350 at 16.944 kb on + strand, within EX28DRAFT_3350 at 16.945 kb on - strand, within EX28DRAFT_3350 at 16.945 kb on - strand, within EX28DRAFT_3350
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media with m-arsenite 1 mM remove 14,695 + EX28DRAFT_3345 0.44 +1.5 14,698 + EX28DRAFT_3345 0.46 +1.2 14,761 + EX28DRAFT_3345 0.89 +0.5 14,774 + -0.3 14,775 - +1.1 14,778 + -0.5 14,783 + -0.3 14,784 - -0.5 14,784 - -0.3 14,784 - +0.1 14,785 - +2.1 14,893 + EX28DRAFT_3346 0.13 +0.0 14,893 + EX28DRAFT_3346 0.13 -0.1 14,893 + EX28DRAFT_3346 0.13 +1.0 14,894 - EX28DRAFT_3346 0.13 -0.5 14,897 + EX28DRAFT_3346 0.14 -0.4 14,897 + EX28DRAFT_3346 0.14 -0.3 14,897 + EX28DRAFT_3346 0.14 -0.1 14,898 - EX28DRAFT_3346 0.14 -0.5 14,898 - EX28DRAFT_3346 0.14 +0.6 14,898 - EX28DRAFT_3346 0.14 +0.8 14,898 - EX28DRAFT_3346 0.14 +0.3 14,899 + EX28DRAFT_3346 0.14 +0.4 14,900 - EX28DRAFT_3346 0.14 -0.1 14,900 - EX28DRAFT_3346 0.14 -0.9 15,222 - EX28DRAFT_3346 0.68 -1.6 15,322 + EX28DRAFT_3346 0.85 +0.1 15,322 + EX28DRAFT_3346 0.85 -0.3 15,413 + +0.8 15,471 + -0.6 15,471 + -0.2 15,492 - +0.5 15,492 - +0.7 15,514 - +0.3 15,523 - -0.1 15,534 + +2.3 15,584 + +0.2 15,654 + +0.5 15,654 + -0.7 15,655 - -0.3 15,657 + +0.1 15,657 + -1.5 15,658 - +0.9 15,658 - +0.6 15,695 + -0.8 15,719 - EX28DRAFT_3348 0.14 +0.2 15,775 + EX28DRAFT_3348 0.31 -3.2 15,775 + EX28DRAFT_3348 0.31 -0.8 15,800 + EX28DRAFT_3348 0.39 +1.1 15,800 + EX28DRAFT_3348 0.39 -0.1 15,800 + EX28DRAFT_3348 0.39 +1.7 15,801 - EX28DRAFT_3348 0.40 +1.0 15,812 - EX28DRAFT_3348 0.43 +0.4 15,849 + EX28DRAFT_3348 0.55 +0.5 16,081 + EX28DRAFT_3349 0.17 +0.2 16,173 + EX28DRAFT_3349 0.35 +0.4 16,283 - EX28DRAFT_3349 0.55 +0.2 16,329 + EX28DRAFT_3349 0.64 -0.3 16,329 + EX28DRAFT_3349 0.64 -0.1 16,349 + EX28DRAFT_3349 0.68 -0.8 16,350 - EX28DRAFT_3349 0.68 -0.1 16,389 + EX28DRAFT_3349 0.75 +0.0 16,390 - EX28DRAFT_3349 0.76 +0.2 16,463 - EX28DRAFT_3349 0.89 -0.1 16,519 + -1.6 16,519 + +0.3 16,520 - +0.7 16,545 + +0.6 16,552 - -2.6 16,656 + +0.4 16,656 + -3.7 16,660 - -0.7 16,660 - +0.0 16,768 - +0.1 16,768 - +1.2 16,768 - +0.2 16,768 - -0.1 16,798 + EX28DRAFT_3350 0.12 -0.2 16,874 - EX28DRAFT_3350 0.19 +1.1 16,919 - EX28DRAFT_3350 0.23 +1.1 16,944 + EX28DRAFT_3350 0.25 -0.8 16,945 - EX28DRAFT_3350 0.25 +0.9 16,945 - EX28DRAFT_3350 0.25 -2.1
Or see this region's nucleotide sequence