Experiment: MS_media with m-arsenite 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_2943 and EX28DRAFT_2944 are separated by 5 nucleotides EX28DRAFT_2944 and EX28DRAFT_2945 are separated by 28 nucleotides EX28DRAFT_2945 and EX28DRAFT_2946 are separated by 101 nucleotides EX28DRAFT_2946 and EX28DRAFT_2947 are separated by 6 nucleotides
EX28DRAFT_2943: EX28DRAFT_2943 - single-stranded-DNA-specific exonuclease RecJ, at 253,527 to 255,260
_2943
EX28DRAFT_2944: EX28DRAFT_2944 - Protein-disulfide isomerase, at 255,266 to 255,979
_2944
EX28DRAFT_2945: EX28DRAFT_2945 - tyrosine recombinase XerD, at 256,008 to 256,904
_2945
EX28DRAFT_2946: EX28DRAFT_2946 - flavodoxin, long chain, at 257,006 to 257,527
_2946
EX28DRAFT_2947: EX28DRAFT_2947 - Protein of unknown function (DUF1434), at 257,534 to 257,944
_2947
Position (kb) 256
257 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 255.268 kb on - strand at 255.272 kb on - strand at 255.503 kb on - strand, within EX28DRAFT_2944 at 255.585 kb on + strand, within EX28DRAFT_2944 at 255.673 kb on + strand, within EX28DRAFT_2944 at 255.763 kb on - strand, within EX28DRAFT_2944 at 255.934 kb on + strand at 255.979 kb on + strand at 256.170 kb on + strand, within EX28DRAFT_2945 at 256.477 kb on + strand, within EX28DRAFT_2945 at 256.478 kb on - strand, within EX28DRAFT_2945 at 256.543 kb on - strand, within EX28DRAFT_2945 at 256.936 kb on + strand at 256.996 kb on + strand at 257.001 kb on - strand at 257.011 kb on + strand at 257.012 kb on - strand at 257.025 kb on - strand at 257.141 kb on + strand, within EX28DRAFT_2946 at 257.142 kb on - strand, within EX28DRAFT_2946 at 257.187 kb on + strand, within EX28DRAFT_2946 at 257.206 kb on + strand, within EX28DRAFT_2946 at 257.206 kb on + strand, within EX28DRAFT_2946 at 257.357 kb on + strand, within EX28DRAFT_2946 at 257.597 kb on - strand, within EX28DRAFT_2947 at 257.738 kb on + strand, within EX28DRAFT_2947 at 257.744 kb on - strand, within EX28DRAFT_2947 at 257.830 kb on + strand, within EX28DRAFT_2947 at 257.831 kb on - strand, within EX28DRAFT_2947 at 257.831 kb on - strand, within EX28DRAFT_2947 at 257.831 kb on - strand, within EX28DRAFT_2947 at 257.831 kb on - strand, within EX28DRAFT_2947 at 257.833 kb on - strand, within EX28DRAFT_2947 at 257.833 kb on - strand, within EX28DRAFT_2947 at 257.888 kb on + strand, within EX28DRAFT_2947
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media with m-arsenite 1 mM remove 255,268 - +1.0 255,272 - -0.0 255,503 - EX28DRAFT_2944 0.33 -0.0 255,585 + EX28DRAFT_2944 0.45 +0.5 255,673 + EX28DRAFT_2944 0.57 -1.1 255,763 - EX28DRAFT_2944 0.70 +1.0 255,934 + -1.3 255,979 + -1.6 256,170 + EX28DRAFT_2945 0.18 -0.8 256,477 + EX28DRAFT_2945 0.52 +0.7 256,478 - EX28DRAFT_2945 0.52 +0.2 256,543 - EX28DRAFT_2945 0.60 -4.7 256,936 + -0.1 256,996 + -0.3 257,001 - -0.5 257,011 + -0.4 257,012 - -3.7 257,025 - -0.3 257,141 + EX28DRAFT_2946 0.26 -4.4 257,142 - EX28DRAFT_2946 0.26 -2.8 257,187 + EX28DRAFT_2946 0.35 -2.2 257,206 + EX28DRAFT_2946 0.38 -4.1 257,206 + EX28DRAFT_2946 0.38 -4.4 257,357 + EX28DRAFT_2946 0.67 -4.1 257,597 - EX28DRAFT_2947 0.15 +0.6 257,738 + EX28DRAFT_2947 0.50 -3.0 257,744 - EX28DRAFT_2947 0.51 +0.6 257,830 + EX28DRAFT_2947 0.72 +0.2 257,831 - EX28DRAFT_2947 0.72 +0.3 257,831 - EX28DRAFT_2947 0.72 +0.3 257,831 - EX28DRAFT_2947 0.72 +0.3 257,831 - EX28DRAFT_2947 0.72 -0.2 257,833 - EX28DRAFT_2947 0.73 +0.9 257,833 - EX28DRAFT_2947 0.73 -1.7 257,888 + EX28DRAFT_2947 0.86 +0.3
Or see this region's nucleotide sequence