Experiment: MS_media with m-arsenite 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_2852 and EX28DRAFT_2853 overlap by 1 nucleotides EX28DRAFT_2853 and EX28DRAFT_2854 are separated by 306 nucleotides EX28DRAFT_2854 and EX28DRAFT_2855 are separated by 85 nucleotides
EX28DRAFT_2852: EX28DRAFT_2852 - sulfite reductase (NADPH) hemoprotein, beta-component, at 152,958 to 154,670
_2852
EX28DRAFT_2853: EX28DRAFT_2853 - sulfite reductase [NADPH] flavoprotein, alpha-component, at 154,670 to 156,475
_2853
EX28DRAFT_2854: EX28DRAFT_2854 - queuosine biosynthesis protein QueD, at 156,782 to 157,144
_2854
EX28DRAFT_2855: EX28DRAFT_2855 - putative 7-cyano-7-deazaguanosine (preQ0) biosynthesis protein QueE, at 157,230 to 157,901
_2855
Position (kb) 154
155
156
157 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 153.745 kb on + strand, within EX28DRAFT_2852 at 153.746 kb on - strand, within EX28DRAFT_2852 at 153.746 kb on - strand, within EX28DRAFT_2852 at 153.746 kb on - strand, within EX28DRAFT_2852 at 153.746 kb on - strand, within EX28DRAFT_2852 at 153.746 kb on - strand, within EX28DRAFT_2852 at 153.872 kb on - strand, within EX28DRAFT_2852 at 153.923 kb on + strand, within EX28DRAFT_2852 at 154.039 kb on + strand, within EX28DRAFT_2852 at 154.040 kb on - strand, within EX28DRAFT_2852 at 154.159 kb on + strand, within EX28DRAFT_2852 at 154.345 kb on + strand, within EX28DRAFT_2852 at 154.499 kb on - strand at 154.499 kb on - strand at 154.499 kb on - strand at 154.503 kb on - strand at 154.632 kb on - strand at 154.821 kb on + strand at 154.821 kb on + strand at 154.822 kb on - strand at 154.822 kb on - strand at 154.822 kb on - strand at 154.822 kb on - strand at 154.822 kb on - strand at 154.886 kb on + strand, within EX28DRAFT_2853 at 154.886 kb on + strand, within EX28DRAFT_2853 at 154.887 kb on - strand, within EX28DRAFT_2853 at 154.887 kb on - strand, within EX28DRAFT_2853 at 154.887 kb on - strand, within EX28DRAFT_2853 at 154.890 kb on + strand, within EX28DRAFT_2853 at 154.890 kb on + strand, within EX28DRAFT_2853 at 154.941 kb on - strand, within EX28DRAFT_2853 at 154.956 kb on + strand, within EX28DRAFT_2853 at 154.957 kb on - strand, within EX28DRAFT_2853 at 155.059 kb on + strand, within EX28DRAFT_2853 at 155.060 kb on - strand, within EX28DRAFT_2853 at 156.003 kb on + strand, within EX28DRAFT_2853 at 156.173 kb on - strand, within EX28DRAFT_2853 at 156.533 kb on + strand at 156.533 kb on + strand at 156.534 kb on - strand at 156.555 kb on - strand at 156.561 kb on + strand at 156.561 kb on + strand at 156.578 kb on + strand at 156.585 kb on + strand at 156.608 kb on + strand at 156.609 kb on - strand at 156.617 kb on + strand at 156.618 kb on - strand at 156.718 kb on + strand at 156.746 kb on + strand at 156.746 kb on + strand at 156.853 kb on + strand, within EX28DRAFT_2854 at 156.854 kb on - strand, within EX28DRAFT_2854 at 156.854 kb on - strand, within EX28DRAFT_2854 at 156.974 kb on + strand, within EX28DRAFT_2854 at 156.975 kb on - strand, within EX28DRAFT_2854 at 156.975 kb on - strand, within EX28DRAFT_2854 at 157.127 kb on - strand at 157.127 kb on - strand at 157.130 kb on + strand at 157.239 kb on - strand at 157.338 kb on + strand, within EX28DRAFT_2855 at 157.427 kb on + strand, within EX28DRAFT_2855 at 157.461 kb on + strand, within EX28DRAFT_2855 at 157.462 kb on - strand, within EX28DRAFT_2855
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media with m-arsenite 1 mM remove 153,745 + EX28DRAFT_2852 0.46 +0.5 153,746 - EX28DRAFT_2852 0.46 -2.9 153,746 - EX28DRAFT_2852 0.46 -2.9 153,746 - EX28DRAFT_2852 0.46 -1.3 153,746 - EX28DRAFT_2852 0.46 -2.5 153,746 - EX28DRAFT_2852 0.46 -0.2 153,872 - EX28DRAFT_2852 0.53 -0.8 153,923 + EX28DRAFT_2852 0.56 +0.3 154,039 + EX28DRAFT_2852 0.63 -2.6 154,040 - EX28DRAFT_2852 0.63 -1.7 154,159 + EX28DRAFT_2852 0.70 -1.3 154,345 + EX28DRAFT_2852 0.81 -4.6 154,499 - -0.4 154,499 - -3.1 154,499 - -2.4 154,503 - -0.6 154,632 - -0.5 154,821 + +1.7 154,821 + -1.1 154,822 - -2.9 154,822 - -4.5 154,822 - -4.1 154,822 - -1.4 154,822 - -2.9 154,886 + EX28DRAFT_2853 0.12 -1.8 154,886 + EX28DRAFT_2853 0.12 -1.7 154,887 - EX28DRAFT_2853 0.12 -2.2 154,887 - EX28DRAFT_2853 0.12 -4.5 154,887 - EX28DRAFT_2853 0.12 -3.7 154,890 + EX28DRAFT_2853 0.12 -2.9 154,890 + EX28DRAFT_2853 0.12 -2.8 154,941 - EX28DRAFT_2853 0.15 -2.5 154,956 + EX28DRAFT_2853 0.16 -3.6 154,957 - EX28DRAFT_2853 0.16 -1.0 155,059 + EX28DRAFT_2853 0.22 -1.1 155,060 - EX28DRAFT_2853 0.22 -4.9 156,003 + EX28DRAFT_2853 0.74 -4.7 156,173 - EX28DRAFT_2853 0.83 -2.4 156,533 + -0.9 156,533 + -3.0 156,534 - -0.3 156,555 - -3.6 156,561 + +1.2 156,561 + +0.7 156,578 + -2.3 156,585 + -3.1 156,608 + -0.5 156,609 - +1.1 156,617 + +0.5 156,618 - +0.3 156,718 + +0.4 156,746 + +1.1 156,746 + +0.8 156,853 + EX28DRAFT_2854 0.20 -0.4 156,854 - EX28DRAFT_2854 0.20 -0.4 156,854 - EX28DRAFT_2854 0.20 -0.4 156,974 + EX28DRAFT_2854 0.53 +0.3 156,975 - EX28DRAFT_2854 0.53 -0.5 156,975 - EX28DRAFT_2854 0.53 -1.2 157,127 - -3.5 157,127 - -0.4 157,130 + +0.1 157,239 - +0.0 157,338 + EX28DRAFT_2855 0.16 -0.7 157,427 + EX28DRAFT_2855 0.29 +0.5 157,461 + EX28DRAFT_2855 0.34 +0.3 157,462 - EX28DRAFT_2855 0.35 +0.3
Or see this region's nucleotide sequence