Experiment: MS_media with m-arsenite 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_2752 and EX28DRAFT_2753 are separated by 9 nucleotides EX28DRAFT_2753 and EX28DRAFT_2754 are separated by 79 nucleotides EX28DRAFT_2754 and EX28DRAFT_2755 are separated by 37 nucleotides EX28DRAFT_2755 and EX28DRAFT_2756 are separated by 495 nucleotides EX28DRAFT_2756 and EX28DRAFT_2757 are separated by 32 nucleotides
EX28DRAFT_2752: EX28DRAFT_2752 - Predicted transcriptional regulators, at 62,579 to 62,878
_2752
EX28DRAFT_2753: EX28DRAFT_2753 - Rhodanese-related sulfurtransferase, at 62,888 to 63,409
_2753
EX28DRAFT_2754: EX28DRAFT_2754 - hypothetical protein, at 63,489 to 63,668
_2754
EX28DRAFT_2755: EX28DRAFT_2755 - DNA-binding protein H-NS, at 63,706 to 64,110
_2755
EX28DRAFT_2756: EX28DRAFT_2756 - Protein of unknown function (DUF1144), at 64,606 to 65,055
_2756
EX28DRAFT_2757: EX28DRAFT_2757 - Protein of unknown function (DUF2002), at 65,088 to 65,432
_2757
Position (kb) 63
64
65 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 62.728 kb on - strand, within EX28DRAFT_2752 at 62.814 kb on - strand, within EX28DRAFT_2752 at 62.835 kb on + strand, within EX28DRAFT_2752 at 62.868 kb on - strand at 63.037 kb on + strand, within EX28DRAFT_2753 at 63.278 kb on + strand, within EX28DRAFT_2753 at 63.414 kb on + strand at 63.426 kb on + strand at 63.485 kb on + strand at 63.486 kb on - strand at 63.486 kb on - strand at 63.486 kb on - strand at 63.488 kb on + strand at 63.507 kb on + strand, within EX28DRAFT_2754 at 63.508 kb on - strand, within EX28DRAFT_2754 at 63.508 kb on - strand, within EX28DRAFT_2754 at 63.553 kb on + strand, within EX28DRAFT_2754 at 63.553 kb on + strand, within EX28DRAFT_2754 at 63.554 kb on - strand, within EX28DRAFT_2754 at 63.606 kb on + strand, within EX28DRAFT_2754 at 63.606 kb on + strand, within EX28DRAFT_2754 at 63.606 kb on + strand, within EX28DRAFT_2754 at 63.607 kb on - strand, within EX28DRAFT_2754 at 63.608 kb on + strand, within EX28DRAFT_2754 at 63.628 kb on + strand, within EX28DRAFT_2754 at 63.822 kb on - strand, within EX28DRAFT_2755 at 63.971 kb on - strand, within EX28DRAFT_2755 at 64.048 kb on + strand, within EX28DRAFT_2755 at 64.084 kb on - strand at 64.087 kb on - strand at 64.114 kb on - strand at 64.114 kb on - strand at 64.144 kb on - strand at 64.147 kb on + strand at 64.148 kb on - strand at 64.182 kb on + strand at 64.214 kb on + strand at 64.215 kb on - strand at 64.218 kb on + strand at 64.221 kb on - strand at 64.294 kb on - strand at 64.500 kb on - strand at 64.519 kb on - strand at 64.525 kb on + strand at 64.697 kb on + strand, within EX28DRAFT_2756 at 64.697 kb on + strand, within EX28DRAFT_2756 at 64.762 kb on - strand, within EX28DRAFT_2756 at 64.765 kb on - strand, within EX28DRAFT_2756 at 64.781 kb on - strand, within EX28DRAFT_2756 at 64.867 kb on + strand, within EX28DRAFT_2756 at 64.868 kb on - strand, within EX28DRAFT_2756 at 65.023 kb on - strand at 65.035 kb on + strand at 65.090 kb on - strand at 65.094 kb on + strand at 65.094 kb on + strand at 65.095 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media with m-arsenite 1 mM remove 62,728 - EX28DRAFT_2752 0.50 +0.6 62,814 - EX28DRAFT_2752 0.78 -0.8 62,835 + EX28DRAFT_2752 0.85 +0.6 62,868 - +0.3 63,037 + EX28DRAFT_2753 0.29 -1.5 63,278 + EX28DRAFT_2753 0.75 +1.0 63,414 + +0.3 63,426 + -0.2 63,485 + +1.2 63,486 - +0.6 63,486 - +0.4 63,486 - +0.8 63,488 + -0.9 63,507 + EX28DRAFT_2754 0.10 +0.3 63,508 - EX28DRAFT_2754 0.11 +0.6 63,508 - EX28DRAFT_2754 0.11 -0.8 63,553 + EX28DRAFT_2754 0.36 -0.1 63,553 + EX28DRAFT_2754 0.36 -2.2 63,554 - EX28DRAFT_2754 0.36 +0.5 63,606 + EX28DRAFT_2754 0.65 -3.1 63,606 + EX28DRAFT_2754 0.65 +0.3 63,606 + EX28DRAFT_2754 0.65 +0.9 63,607 - EX28DRAFT_2754 0.66 +1.1 63,608 + EX28DRAFT_2754 0.66 +0.1 63,628 + EX28DRAFT_2754 0.77 +0.2 63,822 - EX28DRAFT_2755 0.29 +0.4 63,971 - EX28DRAFT_2755 0.65 -0.4 64,048 + EX28DRAFT_2755 0.84 -1.0 64,084 - -2.4 64,087 - -0.6 64,114 - -0.4 64,114 - +0.4 64,144 - -0.4 64,147 + -4.5 64,148 - +0.0 64,182 + +0.0 64,214 + +1.8 64,215 - +1.4 64,218 + +0.2 64,221 - +1.4 64,294 - +0.4 64,500 - +0.4 64,519 - +0.7 64,525 + -0.5 64,697 + EX28DRAFT_2756 0.20 -1.3 64,697 + EX28DRAFT_2756 0.20 +1.8 64,762 - EX28DRAFT_2756 0.35 -0.3 64,765 - EX28DRAFT_2756 0.35 +0.1 64,781 - EX28DRAFT_2756 0.39 +0.1 64,867 + EX28DRAFT_2756 0.58 -0.2 64,868 - EX28DRAFT_2756 0.58 +1.3 65,023 - +0.7 65,035 + -0.1 65,090 - -0.1 65,094 + +0.2 65,094 + +1.7 65,095 - -0.0
Or see this region's nucleotide sequence