Experiment: MS_media with m-arsenite 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_2527 and EX28DRAFT_2528 are separated by 99 nucleotides EX28DRAFT_2528 and EX28DRAFT_2529 are separated by 148 nucleotides EX28DRAFT_2529 and EX28DRAFT_2530 are separated by 212 nucleotides EX28DRAFT_2530 and EX28DRAFT_2531 are separated by 210 nucleotides
EX28DRAFT_2527: EX28DRAFT_2527 - acyl-CoA thioester hydrolase, YbgC/YbaW family, at 2,558,386 to 2,558,784
_2527
EX28DRAFT_2528: EX28DRAFT_2528 - competence protein ComEA helix-hairpin-helix repeat region, at 2,558,884 to 2,559,264
_2528
EX28DRAFT_2529: EX28DRAFT_2529 - Parvulin-like peptidyl-prolyl isomerase, at 2,559,413 to 2,561,284
_2529
EX28DRAFT_2530: EX28DRAFT_2530 - Bacterial nucleoid DNA-binding protein, at 2,561,497 to 2,561,769
_2530
EX28DRAFT_2531: EX28DRAFT_2531 - endopeptidase La, at 2,561,980 to 2,564,334
_2531
Position (kb)
2559
2560
2561
2562 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 2558.527 kb on + strand, within EX28DRAFT_2527 at 2558.528 kb on - strand, within EX28DRAFT_2527 at 2558.810 kb on + strand at 2558.882 kb on - strand at 2558.885 kb on + strand at 2558.885 kb on + strand at 2558.885 kb on + strand at 2558.886 kb on - strand at 2558.975 kb on + strand, within EX28DRAFT_2528 at 2558.975 kb on + strand, within EX28DRAFT_2528 at 2559.066 kb on + strand, within EX28DRAFT_2528 at 2559.270 kb on + strand at 2559.271 kb on - strand at 2559.271 kb on - strand at 2559.313 kb on + strand at 2559.410 kb on + strand at 2559.411 kb on - strand at 2559.411 kb on - strand at 2559.411 kb on - strand at 2559.414 kb on + strand at 2559.414 kb on + strand at 2559.415 kb on - strand at 2559.415 kb on - strand at 2559.931 kb on + strand, within EX28DRAFT_2529 at 2559.932 kb on - strand, within EX28DRAFT_2529 at 2560.134 kb on + strand, within EX28DRAFT_2529 at 2560.258 kb on + strand, within EX28DRAFT_2529 at 2560.258 kb on + strand, within EX28DRAFT_2529 at 2560.258 kb on + strand, within EX28DRAFT_2529 at 2560.259 kb on - strand, within EX28DRAFT_2529 at 2560.367 kb on - strand, within EX28DRAFT_2529 at 2560.580 kb on - strand, within EX28DRAFT_2529 at 2560.581 kb on + strand, within EX28DRAFT_2529 at 2560.581 kb on + strand, within EX28DRAFT_2529 at 2560.581 kb on + strand, within EX28DRAFT_2529 at 2560.633 kb on - strand, within EX28DRAFT_2529 at 2560.636 kb on - strand, within EX28DRAFT_2529 at 2560.831 kb on - strand, within EX28DRAFT_2529 at 2561.163 kb on + strand at 2561.188 kb on - strand at 2561.271 kb on - strand at 2561.290 kb on - strand at 2561.362 kb on - strand at 2561.465 kb on + strand at 2561.466 kb on - strand at 2561.524 kb on - strand at 2561.686 kb on + strand, within EX28DRAFT_2530 at 2561.695 kb on + strand, within EX28DRAFT_2530 at 2561.788 kb on - strand at 2561.795 kb on - strand at 2561.795 kb on - strand at 2561.836 kb on + strand at 2561.858 kb on + strand at 2561.859 kb on - strand at 2561.859 kb on - strand at 2561.859 kb on - strand at 2561.859 kb on - strand at 2561.886 kb on - strand at 2561.886 kb on - strand at 2561.886 kb on - strand at 2561.898 kb on - strand at 2561.902 kb on + strand at 2561.902 kb on + strand at 2561.906 kb on + strand at 2561.907 kb on - strand at 2561.908 kb on + strand at 2561.940 kb on + strand at 2561.941 kb on - strand at 2561.955 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media with m-arsenite 1 mM remove 2,558,527 + EX28DRAFT_2527 0.35 +0.6 2,558,528 - EX28DRAFT_2527 0.36 -1.1 2,558,810 + +0.2 2,558,882 - +0.0 2,558,885 + -0.1 2,558,885 + +0.6 2,558,885 + +0.1 2,558,886 - +1.5 2,558,975 + EX28DRAFT_2528 0.24 -0.3 2,558,975 + EX28DRAFT_2528 0.24 -0.2 2,559,066 + EX28DRAFT_2528 0.48 +0.7 2,559,270 + -0.4 2,559,271 - -1.0 2,559,271 - +0.5 2,559,313 + +0.1 2,559,410 + -0.7 2,559,411 - -0.0 2,559,411 - -3.4 2,559,411 - -0.1 2,559,414 + -0.7 2,559,414 + +0.6 2,559,415 - +0.1 2,559,415 - +0.3 2,559,931 + EX28DRAFT_2529 0.28 +0.2 2,559,932 - EX28DRAFT_2529 0.28 +1.0 2,560,134 + EX28DRAFT_2529 0.39 +0.4 2,560,258 + EX28DRAFT_2529 0.45 +0.4 2,560,258 + EX28DRAFT_2529 0.45 +0.2 2,560,258 + EX28DRAFT_2529 0.45 -1.0 2,560,259 - EX28DRAFT_2529 0.45 -0.5 2,560,367 - EX28DRAFT_2529 0.51 -2.9 2,560,580 - EX28DRAFT_2529 0.62 -0.7 2,560,581 + EX28DRAFT_2529 0.62 -0.5 2,560,581 + EX28DRAFT_2529 0.62 -1.1 2,560,581 + EX28DRAFT_2529 0.62 -2.4 2,560,633 - EX28DRAFT_2529 0.65 -0.4 2,560,636 - EX28DRAFT_2529 0.65 -2.3 2,560,831 - EX28DRAFT_2529 0.76 -4.9 2,561,163 + -0.6 2,561,188 - -1.1 2,561,271 - +0.3 2,561,290 - -0.5 2,561,362 - -0.6 2,561,465 + -0.4 2,561,466 - -0.8 2,561,524 - -0.4 2,561,686 + EX28DRAFT_2530 0.69 -2.5 2,561,695 + EX28DRAFT_2530 0.73 -0.1 2,561,788 - -1.0 2,561,795 - -2.2 2,561,795 - -0.9 2,561,836 + -1.2 2,561,858 + -0.6 2,561,859 - -4.4 2,561,859 - -0.0 2,561,859 - -4.7 2,561,859 - -1.8 2,561,886 - -1.5 2,561,886 - -0.4 2,561,886 - -0.5 2,561,898 - -1.3 2,561,902 + +0.1 2,561,902 + +0.2 2,561,906 + -3.5 2,561,907 - -1.2 2,561,908 + +0.0 2,561,940 + +0.4 2,561,941 - -1.5 2,561,955 + +0.3
Or see this region's nucleotide sequence