Experiment: MS_media with m-arsenite 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_2393 and EX28DRAFT_2394 are separated by 33 nucleotides EX28DRAFT_2394 and EX28DRAFT_2395 are separated by 201 nucleotides EX28DRAFT_2395 and EX28DRAFT_2396 are separated by 70 nucleotides
EX28DRAFT_2393: EX28DRAFT_2393 - Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase], at 2,418,261 to 2,419,955
_2393
EX28DRAFT_2394: EX28DRAFT_2394 - Acetyltransferases, including N-acetylases of ribosomal proteins, at 2,419,989 to 2,420,687
_2394
EX28DRAFT_2395: EX28DRAFT_2395 - ABC-type dipeptide transport system, periplasmic component, at 2,420,889 to 2,422,457
_2395
EX28DRAFT_2396: EX28DRAFT_2396 - ABC-type dipeptide/oligopeptide/nickel transport systems, permease components, at 2,422,528 to 2,423,541
_2396
Position (kb)
2420
2421
2422
2423 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 2419.972 kb on + strand at 2419.972 kb on + strand at 2419.978 kb on - strand at 2419.981 kb on + strand at 2419.981 kb on + strand at 2419.982 kb on - strand at 2419.982 kb on - strand at 2419.982 kb on - strand at 2419.982 kb on - strand at 2419.990 kb on + strand at 2419.990 kb on + strand at 2419.991 kb on - strand at 2419.991 kb on - strand at 2419.993 kb on - strand at 2420.015 kb on + strand at 2420.103 kb on - strand, within EX28DRAFT_2394 at 2420.103 kb on - strand, within EX28DRAFT_2394 at 2420.272 kb on - strand, within EX28DRAFT_2394 at 2420.371 kb on + strand, within EX28DRAFT_2394 at 2420.371 kb on + strand, within EX28DRAFT_2394 at 2420.371 kb on + strand, within EX28DRAFT_2394 at 2420.372 kb on - strand, within EX28DRAFT_2394 at 2420.372 kb on - strand, within EX28DRAFT_2394 at 2420.372 kb on - strand, within EX28DRAFT_2394 at 2420.372 kb on - strand, within EX28DRAFT_2394 at 2420.372 kb on - strand, within EX28DRAFT_2394 at 2420.543 kb on - strand, within EX28DRAFT_2394 at 2420.617 kb on - strand, within EX28DRAFT_2394 at 2420.620 kb on - strand at 2420.723 kb on - strand at 2420.731 kb on - strand at 2420.758 kb on + strand at 2420.792 kb on + strand at 2420.792 kb on + strand at 2420.794 kb on + strand at 2420.794 kb on + strand at 2420.795 kb on - strand at 2420.827 kb on - strand at 2420.853 kb on + strand at 2420.867 kb on + strand at 2420.867 kb on + strand at 2420.868 kb on - strand at 2420.870 kb on - strand at 2421.070 kb on + strand, within EX28DRAFT_2395 at 2421.165 kb on - strand, within EX28DRAFT_2395 at 2421.226 kb on - strand, within EX28DRAFT_2395 at 2421.381 kb on - strand, within EX28DRAFT_2395 at 2421.676 kb on + strand, within EX28DRAFT_2395 at 2421.677 kb on - strand, within EX28DRAFT_2395 at 2421.859 kb on - strand, within EX28DRAFT_2395 at 2422.098 kb on + strand, within EX28DRAFT_2395 at 2422.309 kb on - strand at 2422.367 kb on + strand at 2422.679 kb on - strand, within EX28DRAFT_2396 at 2422.680 kb on - strand, within EX28DRAFT_2396 at 2423.058 kb on + strand, within EX28DRAFT_2396 at 2423.095 kb on - strand, within EX28DRAFT_2396 at 2423.171 kb on - strand, within EX28DRAFT_2396 at 2423.173 kb on + strand, within EX28DRAFT_2396 at 2423.236 kb on + strand, within EX28DRAFT_2396 at 2423.237 kb on - strand, within EX28DRAFT_2396 at 2423.274 kb on - strand, within EX28DRAFT_2396
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media with m-arsenite 1 mM remove 2,419,972 + +0.5 2,419,972 + -4.9 2,419,978 - -1.1 2,419,981 + -0.2 2,419,981 + -0.1 2,419,982 - -0.8 2,419,982 - +0.9 2,419,982 - -3.9 2,419,982 - +0.2 2,419,990 + -1.9 2,419,990 + +0.6 2,419,991 - +0.3 2,419,991 - +1.9 2,419,993 - +1.2 2,420,015 + +0.1 2,420,103 - EX28DRAFT_2394 0.16 +0.1 2,420,103 - EX28DRAFT_2394 0.16 -1.3 2,420,272 - EX28DRAFT_2394 0.40 -2.2 2,420,371 + EX28DRAFT_2394 0.55 +0.1 2,420,371 + EX28DRAFT_2394 0.55 -1.5 2,420,371 + EX28DRAFT_2394 0.55 -0.0 2,420,372 - EX28DRAFT_2394 0.55 -0.2 2,420,372 - EX28DRAFT_2394 0.55 -0.3 2,420,372 - EX28DRAFT_2394 0.55 +0.3 2,420,372 - EX28DRAFT_2394 0.55 +0.3 2,420,372 - EX28DRAFT_2394 0.55 -0.2 2,420,543 - EX28DRAFT_2394 0.79 -0.8 2,420,617 - EX28DRAFT_2394 0.90 -0.2 2,420,620 - -4.6 2,420,723 - -1.7 2,420,731 - +0.4 2,420,758 + -0.1 2,420,792 + -1.1 2,420,792 + -0.0 2,420,794 + -0.1 2,420,794 + +0.1 2,420,795 - -3.6 2,420,827 - -1.0 2,420,853 + -0.5 2,420,867 + -0.2 2,420,867 + -0.8 2,420,868 - +0.2 2,420,870 - +0.1 2,421,070 + EX28DRAFT_2395 0.12 -1.8 2,421,165 - EX28DRAFT_2395 0.18 -0.5 2,421,226 - EX28DRAFT_2395 0.21 +0.6 2,421,381 - EX28DRAFT_2395 0.31 -3.2 2,421,676 + EX28DRAFT_2395 0.50 +0.5 2,421,677 - EX28DRAFT_2395 0.50 -0.8 2,421,859 - EX28DRAFT_2395 0.62 -0.3 2,422,098 + EX28DRAFT_2395 0.77 -0.5 2,422,309 - +0.7 2,422,367 + +0.2 2,422,679 - EX28DRAFT_2396 0.15 +1.3 2,422,680 - EX28DRAFT_2396 0.15 -2.4 2,423,058 + EX28DRAFT_2396 0.52 +0.5 2,423,095 - EX28DRAFT_2396 0.56 -4.3 2,423,171 - EX28DRAFT_2396 0.63 -0.3 2,423,173 + EX28DRAFT_2396 0.64 -1.4 2,423,236 + EX28DRAFT_2396 0.70 -0.1 2,423,237 - EX28DRAFT_2396 0.70 -4.6 2,423,274 - EX28DRAFT_2396 0.74 -0.3
Or see this region's nucleotide sequence