Experiment: MS_media with m-arsenite 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_2269 and EX28DRAFT_2270 overlap by 7 nucleotides EX28DRAFT_2270 and EX28DRAFT_2271 are separated by 263 nucleotides EX28DRAFT_2271 and EX28DRAFT_2272 are separated by 1 nucleotides
EX28DRAFT_2269: EX28DRAFT_2269 - succinate dehydrogenase, hydrophobic membrane anchor protein, at 2,294,251 to 2,294,598
_2269
EX28DRAFT_2270: EX28DRAFT_2270 - succinate dehydrogenase subunit C (EC 1.3.5.1), at 2,294,592 to 2,295,071
_2270
EX28DRAFT_2271: EX28DRAFT_2271 - hypothetical protein, at 2,295,335 to 2,295,634
_2271
EX28DRAFT_2272: EX28DRAFT_2272 - citrate synthase (EC 2.3.3.1), at 2,295,636 to 2,296,919
_2272
Position (kb)
2295
2296 Strain fitness (log2 ratio)
-7
-6
-5
-4
-3
-2
-1
0
1
2
3 at 2294.352 kb on - strand, within EX28DRAFT_2269 at 2294.352 kb on - strand, within EX28DRAFT_2269 at 2294.502 kb on + strand, within EX28DRAFT_2269 at 2294.504 kb on + strand, within EX28DRAFT_2269 at 2294.504 kb on + strand, within EX28DRAFT_2269 at 2294.504 kb on + strand, within EX28DRAFT_2269 at 2294.505 kb on - strand, within EX28DRAFT_2269 at 2294.513 kb on + strand, within EX28DRAFT_2269 at 2294.513 kb on + strand, within EX28DRAFT_2269 at 2294.513 kb on + strand, within EX28DRAFT_2269 at 2294.560 kb on + strand, within EX28DRAFT_2269 at 2294.601 kb on - strand at 2294.690 kb on - strand, within EX28DRAFT_2270 at 2295.107 kb on + strand at 2295.193 kb on - strand at 2295.196 kb on + strand at 2295.196 kb on + strand at 2295.197 kb on - strand at 2295.197 kb on - strand at 2295.298 kb on - strand at 2295.321 kb on - strand at 2295.322 kb on + strand at 2295.322 kb on + strand at 2295.322 kb on + strand at 2295.322 kb on + strand at 2295.323 kb on - strand at 2295.323 kb on - strand at 2295.367 kb on + strand, within EX28DRAFT_2271 at 2295.368 kb on - strand, within EX28DRAFT_2271 at 2295.371 kb on + strand, within EX28DRAFT_2271 at 2295.411 kb on - strand, within EX28DRAFT_2271 at 2295.413 kb on + strand, within EX28DRAFT_2271 at 2295.414 kb on - strand, within EX28DRAFT_2271 at 2295.422 kb on - strand, within EX28DRAFT_2271 at 2295.422 kb on - strand, within EX28DRAFT_2271 at 2295.506 kb on - strand, within EX28DRAFT_2271 at 2295.552 kb on + strand, within EX28DRAFT_2271 at 2295.552 kb on + strand, within EX28DRAFT_2271 at 2295.573 kb on + strand, within EX28DRAFT_2271 at 2295.608 kb on + strand at 2295.633 kb on + strand at 2295.644 kb on + strand at 2295.644 kb on + strand at 2295.645 kb on - strand at 2295.697 kb on + strand at 2295.697 kb on + strand at 2295.698 kb on - strand at 2295.722 kb on + strand at 2295.776 kb on + strand, within EX28DRAFT_2272 at 2295.810 kb on + strand, within EX28DRAFT_2272 at 2295.897 kb on + strand, within EX28DRAFT_2272 at 2295.897 kb on + strand, within EX28DRAFT_2272 at 2295.898 kb on - strand, within EX28DRAFT_2272 at 2295.898 kb on - strand, within EX28DRAFT_2272 at 2295.898 kb on - strand, within EX28DRAFT_2272 at 2295.898 kb on - strand, within EX28DRAFT_2272 at 2295.937 kb on - strand, within EX28DRAFT_2272 at 2296.072 kb on - strand, within EX28DRAFT_2272 at 2296.095 kb on - strand, within EX28DRAFT_2272 at 2296.170 kb on + strand, within EX28DRAFT_2272 at 2296.170 kb on + strand, within EX28DRAFT_2272 at 2296.170 kb on + strand, within EX28DRAFT_2272 at 2296.171 kb on - strand, within EX28DRAFT_2272 at 2296.171 kb on - strand, within EX28DRAFT_2272 at 2296.194 kb on + strand, within EX28DRAFT_2272 at 2296.215 kb on + strand, within EX28DRAFT_2272 at 2296.216 kb on - strand, within EX28DRAFT_2272
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media with m-arsenite 1 mM remove 2,294,352 - EX28DRAFT_2269 0.29 +2.7 2,294,352 - EX28DRAFT_2269 0.29 +2.4 2,294,502 + EX28DRAFT_2269 0.72 +2.6 2,294,504 + EX28DRAFT_2269 0.73 +3.2 2,294,504 + EX28DRAFT_2269 0.73 +2.0 2,294,504 + EX28DRAFT_2269 0.73 +1.6 2,294,505 - EX28DRAFT_2269 0.73 +3.1 2,294,513 + EX28DRAFT_2269 0.75 +2.6 2,294,513 + EX28DRAFT_2269 0.75 +3.7 2,294,513 + EX28DRAFT_2269 0.75 +3.1 2,294,560 + EX28DRAFT_2269 0.89 +1.3 2,294,601 - +3.3 2,294,690 - EX28DRAFT_2270 0.20 +2.7 2,295,107 + +2.1 2,295,193 - +1.1 2,295,196 + -2.1 2,295,196 + -0.1 2,295,197 - +0.2 2,295,197 - -0.1 2,295,298 - -0.3 2,295,321 - -1.3 2,295,322 + -0.9 2,295,322 + -0.3 2,295,322 + -0.4 2,295,322 + -0.3 2,295,323 - -0.6 2,295,323 - -0.3 2,295,367 + EX28DRAFT_2271 0.11 -0.3 2,295,368 - EX28DRAFT_2271 0.11 -0.6 2,295,371 + EX28DRAFT_2271 0.12 -1.4 2,295,411 - EX28DRAFT_2271 0.25 -3.1 2,295,413 + EX28DRAFT_2271 0.26 -1.3 2,295,414 - EX28DRAFT_2271 0.26 -3.5 2,295,422 - EX28DRAFT_2271 0.29 -1.0 2,295,422 - EX28DRAFT_2271 0.29 -1.2 2,295,506 - EX28DRAFT_2271 0.57 -4.2 2,295,552 + EX28DRAFT_2271 0.72 -4.3 2,295,552 + EX28DRAFT_2271 0.72 -3.7 2,295,573 + EX28DRAFT_2271 0.79 -2.0 2,295,608 + -2.1 2,295,633 + -0.9 2,295,644 + -2.4 2,295,644 + -1.4 2,295,645 - -3.5 2,295,697 + -6.2 2,295,697 + -3.8 2,295,698 - -5.1 2,295,722 + -1.1 2,295,776 + EX28DRAFT_2272 0.11 -3.5 2,295,810 + EX28DRAFT_2272 0.14 -0.1 2,295,897 + EX28DRAFT_2272 0.20 -3.2 2,295,897 + EX28DRAFT_2272 0.20 -3.8 2,295,898 - EX28DRAFT_2272 0.20 -6.8 2,295,898 - EX28DRAFT_2272 0.20 -4.0 2,295,898 - EX28DRAFT_2272 0.20 -3.2 2,295,898 - EX28DRAFT_2272 0.20 -5.3 2,295,937 - EX28DRAFT_2272 0.23 -2.5 2,296,072 - EX28DRAFT_2272 0.34 -0.3 2,296,095 - EX28DRAFT_2272 0.36 -2.7 2,296,170 + EX28DRAFT_2272 0.42 -4.1 2,296,170 + EX28DRAFT_2272 0.42 -3.7 2,296,170 + EX28DRAFT_2272 0.42 -4.2 2,296,171 - EX28DRAFT_2272 0.42 -2.4 2,296,171 - EX28DRAFT_2272 0.42 -1.1 2,296,194 + EX28DRAFT_2272 0.43 -3.1 2,296,215 + EX28DRAFT_2272 0.45 -3.3 2,296,216 - EX28DRAFT_2272 0.45 -1.9
Or see this region's nucleotide sequence