Experiment: MS_media with m-arsenite 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_1714 and EX28DRAFT_1715 are separated by 294 nucleotides EX28DRAFT_1715 and EX28DRAFT_1716 are separated by 74 nucleotides EX28DRAFT_1716 and EX28DRAFT_1717 overlap by 1 nucleotides EX28DRAFT_1717 and EX28DRAFT_1718 are separated by 34 nucleotides
EX28DRAFT_1714: EX28DRAFT_1714 - FOG: EAL domain, at 1,730,968 to 1,731,684
_1714
EX28DRAFT_1715: EX28DRAFT_1715 - Cell wall-associated hydrolases (invasion-associated proteins), at 1,731,979 to 1,732,443
_1715
EX28DRAFT_1716: EX28DRAFT_1716 - ABC-type cobalamin transport system, ATPase component, at 1,732,518 to 1,733,273
_1716
EX28DRAFT_1717: EX28DRAFT_1717 - Glutathione peroxidase, at 1,733,273 to 1,733,824
_1717
EX28DRAFT_1718: EX28DRAFT_1718 - ABC-type cobalamin transport system, permease component, at 1,733,859 to 1,734,839
_1718
Position (kb)
1732
1733
1734 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1731.840 kb on + strand at 1731.938 kb on - strand at 1731.940 kb on + strand at 1731.944 kb on + strand at 1731.957 kb on + strand at 1731.958 kb on - strand at 1731.980 kb on + strand at 1731.981 kb on - strand at 1731.983 kb on - strand at 1731.983 kb on - strand at 1731.983 kb on - strand at 1731.983 kb on - strand at 1731.985 kb on - strand at 1731.985 kb on - strand at 1731.985 kb on - strand at 1731.985 kb on - strand at 1731.985 kb on - strand at 1732.181 kb on - strand, within EX28DRAFT_1715 at 1732.217 kb on - strand, within EX28DRAFT_1715 at 1732.217 kb on - strand, within EX28DRAFT_1715 at 1732.217 kb on - strand, within EX28DRAFT_1715 at 1732.227 kb on + strand, within EX28DRAFT_1715 at 1732.520 kb on - strand at 1732.533 kb on - strand at 1732.547 kb on + strand at 1732.547 kb on + strand at 1732.547 kb on + strand at 1732.547 kb on + strand at 1732.548 kb on - strand at 1732.558 kb on - strand at 1732.793 kb on + strand, within EX28DRAFT_1716 at 1732.793 kb on + strand, within EX28DRAFT_1716 at 1732.830 kb on - strand, within EX28DRAFT_1716 at 1732.830 kb on - strand, within EX28DRAFT_1716 at 1732.920 kb on - strand, within EX28DRAFT_1716 at 1733.045 kb on - strand, within EX28DRAFT_1716 at 1733.274 kb on + strand at 1733.566 kb on - strand, within EX28DRAFT_1717 at 1733.588 kb on - strand, within EX28DRAFT_1717 at 1733.738 kb on - strand, within EX28DRAFT_1717 at 1733.738 kb on - strand, within EX28DRAFT_1717 at 1733.875 kb on - strand at 1733.878 kb on - strand at 1733.878 kb on - strand at 1733.878 kb on - strand at 1733.888 kb on + strand at 1733.967 kb on - strand, within EX28DRAFT_1718 at 1734.126 kb on - strand, within EX28DRAFT_1718 at 1734.126 kb on - strand, within EX28DRAFT_1718 at 1734.129 kb on + strand, within EX28DRAFT_1718 at 1734.130 kb on - strand, within EX28DRAFT_1718 at 1734.130 kb on - strand, within EX28DRAFT_1718 at 1734.130 kb on - strand, within EX28DRAFT_1718 at 1734.130 kb on - strand, within EX28DRAFT_1718 at 1734.130 kb on - strand, within EX28DRAFT_1718 at 1734.130 kb on - strand, within EX28DRAFT_1718 at 1734.132 kb on + strand, within EX28DRAFT_1718 at 1734.132 kb on + strand, within EX28DRAFT_1718 at 1734.133 kb on - strand, within EX28DRAFT_1718 at 1734.203 kb on + strand, within EX28DRAFT_1718 at 1734.204 kb on - strand, within EX28DRAFT_1718 at 1734.204 kb on - strand, within EX28DRAFT_1718
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media with m-arsenite 1 mM remove 1,731,840 + -0.4 1,731,938 - -0.1 1,731,940 + -4.6 1,731,944 + +0.4 1,731,957 + +0.5 1,731,958 - +0.0 1,731,980 + +0.9 1,731,981 - +0.7 1,731,983 - +1.1 1,731,983 - -3.9 1,731,983 - +0.1 1,731,983 - +0.2 1,731,985 - +0.2 1,731,985 - +1.2 1,731,985 - +0.5 1,731,985 - -0.9 1,731,985 - +0.7 1,732,181 - EX28DRAFT_1715 0.43 +1.1 1,732,217 - EX28DRAFT_1715 0.51 +1.9 1,732,217 - EX28DRAFT_1715 0.51 -0.7 1,732,217 - EX28DRAFT_1715 0.51 -1.1 1,732,227 + EX28DRAFT_1715 0.53 +0.3 1,732,520 - -0.3 1,732,533 - +0.9 1,732,547 + -1.6 1,732,547 + +0.3 1,732,547 + +0.1 1,732,547 + +0.7 1,732,548 - +0.1 1,732,558 - +0.2 1,732,793 + EX28DRAFT_1716 0.36 +0.5 1,732,793 + EX28DRAFT_1716 0.36 -3.7 1,732,830 - EX28DRAFT_1716 0.41 +0.1 1,732,830 - EX28DRAFT_1716 0.41 -0.1 1,732,920 - EX28DRAFT_1716 0.53 +0.4 1,733,045 - EX28DRAFT_1716 0.70 +1.2 1,733,274 + +0.1 1,733,566 - EX28DRAFT_1717 0.53 -0.7 1,733,588 - EX28DRAFT_1717 0.57 -0.9 1,733,738 - EX28DRAFT_1717 0.84 +0.3 1,733,738 - EX28DRAFT_1717 0.84 -0.4 1,733,875 - +0.4 1,733,878 - +0.6 1,733,878 - -1.5 1,733,878 - +1.9 1,733,888 + +0.8 1,733,967 - EX28DRAFT_1718 0.11 +0.3 1,734,126 - EX28DRAFT_1718 0.27 -4.1 1,734,126 - EX28DRAFT_1718 0.27 -0.6 1,734,129 + EX28DRAFT_1718 0.28 +1.4 1,734,130 - EX28DRAFT_1718 0.28 -0.6 1,734,130 - EX28DRAFT_1718 0.28 -1.2 1,734,130 - EX28DRAFT_1718 0.28 +1.0 1,734,130 - EX28DRAFT_1718 0.28 -1.6 1,734,130 - EX28DRAFT_1718 0.28 -0.0 1,734,130 - EX28DRAFT_1718 0.28 +1.2 1,734,132 + EX28DRAFT_1718 0.28 -1.0 1,734,132 + EX28DRAFT_1718 0.28 +0.5 1,734,133 - EX28DRAFT_1718 0.28 +0.5 1,734,203 + EX28DRAFT_1718 0.35 +1.8 1,734,204 - EX28DRAFT_1718 0.35 +0.5 1,734,204 - EX28DRAFT_1718 0.35 +0.3
Or see this region's nucleotide sequence