Experiment: MS_media with m-arsenite 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_1638 and EX28DRAFT_1639 are separated by 35 nucleotides EX28DRAFT_1639 and EX28DRAFT_1640 are separated by 192 nucleotides EX28DRAFT_1640 and EX28DRAFT_1641 overlap by 34 nucleotides EX28DRAFT_1641 and EX28DRAFT_1642 overlap by 1 nucleotides
EX28DRAFT_1638: EX28DRAFT_1638 - diguanylate cyclase (GGDEF) domain, at 1,655,421 to 1,656,782
_1638
EX28DRAFT_1639: EX28DRAFT_1639 - Transcriptional regulators, at 1,656,818 to 1,657,252
_1639
EX28DRAFT_1640: EX28DRAFT_1640 - Protein of unknown function (DUF1656), at 1,657,445 to 1,657,681
_1640
EX28DRAFT_1641: EX28DRAFT_1641 - RND family efflux transporter, MFP subunit, at 1,657,648 to 1,658,544
_1641
EX28DRAFT_1642: EX28DRAFT_1642 - Predicted membrane protein, at 1,658,544 to 1,660,577
_1642
Position (kb)
1657
1658
1659 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1656.735 kb on + strand at 1656.735 kb on + strand at 1656.736 kb on - strand at 1656.736 kb on - strand at 1656.780 kb on + strand at 1656.834 kb on - strand at 1656.958 kb on + strand, within EX28DRAFT_1639 at 1657.215 kb on - strand at 1657.238 kb on + strand at 1657.302 kb on + strand at 1657.356 kb on + strand at 1657.357 kb on - strand at 1657.357 kb on - strand at 1657.357 kb on - strand at 1657.369 kb on + strand at 1657.369 kb on + strand at 1657.369 kb on + strand at 1657.370 kb on - strand at 1657.370 kb on - strand at 1657.370 kb on - strand at 1657.370 kb on - strand at 1657.395 kb on - strand at 1657.395 kb on - strand at 1657.397 kb on + strand at 1657.398 kb on - strand at 1657.509 kb on - strand, within EX28DRAFT_1640 at 1657.526 kb on - strand, within EX28DRAFT_1640 at 1657.589 kb on + strand, within EX28DRAFT_1640 at 1657.590 kb on - strand, within EX28DRAFT_1640 at 1657.590 kb on - strand, within EX28DRAFT_1640 at 1657.770 kb on + strand, within EX28DRAFT_1641 at 1657.770 kb on + strand, within EX28DRAFT_1641 at 1658.026 kb on + strand, within EX28DRAFT_1641 at 1658.027 kb on - strand, within EX28DRAFT_1641 at 1658.045 kb on + strand, within EX28DRAFT_1641 at 1658.197 kb on + strand, within EX28DRAFT_1641 at 1658.197 kb on + strand, within EX28DRAFT_1641 at 1658.197 kb on + strand, within EX28DRAFT_1641 at 1658.198 kb on - strand, within EX28DRAFT_1641 at 1658.198 kb on - strand, within EX28DRAFT_1641 at 1658.198 kb on - strand, within EX28DRAFT_1641 at 1658.198 kb on - strand, within EX28DRAFT_1641 at 1658.248 kb on + strand, within EX28DRAFT_1641 at 1658.320 kb on + strand, within EX28DRAFT_1641 at 1658.320 kb on + strand, within EX28DRAFT_1641 at 1658.321 kb on - strand, within EX28DRAFT_1641 at 1658.417 kb on + strand, within EX28DRAFT_1641 at 1658.658 kb on + strand at 1658.659 kb on - strand at 1658.682 kb on + strand at 1658.742 kb on - strand at 1658.791 kb on + strand, within EX28DRAFT_1642 at 1658.796 kb on - strand, within EX28DRAFT_1642 at 1658.886 kb on - strand, within EX28DRAFT_1642 at 1659.266 kb on + strand, within EX28DRAFT_1642 at 1659.266 kb on + strand, within EX28DRAFT_1642 at 1659.270 kb on + strand, within EX28DRAFT_1642 at 1659.270 kb on + strand, within EX28DRAFT_1642 at 1659.270 kb on + strand, within EX28DRAFT_1642 at 1659.270 kb on + strand, within EX28DRAFT_1642 at 1659.414 kb on + strand, within EX28DRAFT_1642 at 1659.415 kb on - strand, within EX28DRAFT_1642 at 1659.460 kb on - strand, within EX28DRAFT_1642 at 1659.460 kb on - strand, within EX28DRAFT_1642 at 1659.513 kb on + strand, within EX28DRAFT_1642 at 1659.513 kb on + strand, within EX28DRAFT_1642 at 1659.514 kb on - strand, within EX28DRAFT_1642 at 1659.537 kb on - strand, within EX28DRAFT_1642
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media with m-arsenite 1 mM remove 1,656,735 + +0.2 1,656,735 + +0.6 1,656,736 - -0.4 1,656,736 - -0.9 1,656,780 + +0.2 1,656,834 - +0.2 1,656,958 + EX28DRAFT_1639 0.32 +2.1 1,657,215 - +0.8 1,657,238 + +0.3 1,657,302 + +1.4 1,657,356 + +0.1 1,657,357 - +0.2 1,657,357 - -0.1 1,657,357 - -0.9 1,657,369 + -0.3 1,657,369 + +1.1 1,657,369 + -2.2 1,657,370 - -2.9 1,657,370 - -0.7 1,657,370 - +0.2 1,657,370 - -3.1 1,657,395 - +0.9 1,657,395 - -0.3 1,657,397 + -0.5 1,657,398 - -1.1 1,657,509 - EX28DRAFT_1640 0.27 -0.3 1,657,526 - EX28DRAFT_1640 0.34 +3.0 1,657,589 + EX28DRAFT_1640 0.61 -2.1 1,657,590 - EX28DRAFT_1640 0.61 -0.3 1,657,590 - EX28DRAFT_1640 0.61 -2.8 1,657,770 + EX28DRAFT_1641 0.14 -0.1 1,657,770 + EX28DRAFT_1641 0.14 -1.6 1,658,026 + EX28DRAFT_1641 0.42 +2.1 1,658,027 - EX28DRAFT_1641 0.42 +0.7 1,658,045 + EX28DRAFT_1641 0.44 -0.8 1,658,197 + EX28DRAFT_1641 0.61 +0.7 1,658,197 + EX28DRAFT_1641 0.61 +1.1 1,658,197 + EX28DRAFT_1641 0.61 -0.2 1,658,198 - EX28DRAFT_1641 0.61 -0.2 1,658,198 - EX28DRAFT_1641 0.61 +0.3 1,658,198 - EX28DRAFT_1641 0.61 -3.2 1,658,198 - EX28DRAFT_1641 0.61 -1.3 1,658,248 + EX28DRAFT_1641 0.67 +1.0 1,658,320 + EX28DRAFT_1641 0.75 -1.7 1,658,320 + EX28DRAFT_1641 0.75 -0.7 1,658,321 - EX28DRAFT_1641 0.75 -0.2 1,658,417 + EX28DRAFT_1641 0.86 -0.4 1,658,658 + +0.0 1,658,659 - -1.0 1,658,682 + -0.9 1,658,742 - -0.4 1,658,791 + EX28DRAFT_1642 0.12 -0.2 1,658,796 - EX28DRAFT_1642 0.12 -0.1 1,658,886 - EX28DRAFT_1642 0.17 +0.9 1,659,266 + EX28DRAFT_1642 0.35 -0.2 1,659,266 + EX28DRAFT_1642 0.35 -0.2 1,659,270 + EX28DRAFT_1642 0.36 -0.1 1,659,270 + EX28DRAFT_1642 0.36 +0.1 1,659,270 + EX28DRAFT_1642 0.36 -1.0 1,659,270 + EX28DRAFT_1642 0.36 +0.5 1,659,414 + EX28DRAFT_1642 0.43 +0.1 1,659,415 - EX28DRAFT_1642 0.43 +0.1 1,659,460 - EX28DRAFT_1642 0.45 -0.0 1,659,460 - EX28DRAFT_1642 0.45 -0.6 1,659,513 + EX28DRAFT_1642 0.48 +1.1 1,659,513 + EX28DRAFT_1642 0.48 +0.0 1,659,514 - EX28DRAFT_1642 0.48 -1.0 1,659,537 - EX28DRAFT_1642 0.49 -0.1
Or see this region's nucleotide sequence