Experiment: MS_media with m-arsenite 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_1205 and EX28DRAFT_1206 are separated by 20 nucleotides EX28DRAFT_1206 and EX28DRAFT_1207 are separated by 32 nucleotides EX28DRAFT_1207 and EX28DRAFT_1208 are separated by 33 nucleotides
EX28DRAFT_1205: EX28DRAFT_1205 - Transcriptional regulators, at 1,225,139 to 1,225,573
_1205
EX28DRAFT_1206: EX28DRAFT_1206 - Helix-turn-helix domain, at 1,225,594 to 1,225,974
_1206
EX28DRAFT_1207: EX28DRAFT_1207 - MarB protein, at 1,226,007 to 1,226,225
_1207
EX28DRAFT_1208: EX28DRAFT_1208 - Predicted permease, DMT superfamily, at 1,226,259 to 1,227,161
_1208
Position (kb)
1226
1227 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 1225.100 kb on - strand at 1225.190 kb on - strand, within EX28DRAFT_1205 at 1225.190 kb on - strand, within EX28DRAFT_1205 at 1225.196 kb on - strand, within EX28DRAFT_1205 at 1225.200 kb on + strand, within EX28DRAFT_1205 at 1225.232 kb on + strand, within EX28DRAFT_1205 at 1225.232 kb on + strand, within EX28DRAFT_1205 at 1225.300 kb on + strand, within EX28DRAFT_1205 at 1225.300 kb on + strand, within EX28DRAFT_1205 at 1225.301 kb on - strand, within EX28DRAFT_1205 at 1225.301 kb on - strand, within EX28DRAFT_1205 at 1225.301 kb on - strand, within EX28DRAFT_1205 at 1225.303 kb on + strand, within EX28DRAFT_1205 at 1225.304 kb on - strand, within EX28DRAFT_1205 at 1225.304 kb on - strand, within EX28DRAFT_1205 at 1225.304 kb on - strand, within EX28DRAFT_1205 at 1225.389 kb on + strand, within EX28DRAFT_1205 at 1225.426 kb on - strand, within EX28DRAFT_1205 at 1225.479 kb on + strand, within EX28DRAFT_1205 at 1225.507 kb on - strand, within EX28DRAFT_1205 at 1225.587 kb on + strand at 1225.608 kb on + strand at 1225.609 kb on - strand at 1225.754 kb on + strand, within EX28DRAFT_1206 at 1225.762 kb on + strand, within EX28DRAFT_1206 at 1225.763 kb on - strand, within EX28DRAFT_1206 at 1225.828 kb on + strand, within EX28DRAFT_1206 at 1225.828 kb on + strand, within EX28DRAFT_1206 at 1225.913 kb on - strand, within EX28DRAFT_1206 at 1225.945 kb on + strand at 1225.952 kb on - strand at 1225.968 kb on + strand at 1225.969 kb on - strand at 1225.972 kb on + strand at 1225.973 kb on - strand at 1225.973 kb on - strand at 1225.985 kb on + strand at 1226.100 kb on - strand, within EX28DRAFT_1207 at 1226.100 kb on - strand, within EX28DRAFT_1207 at 1226.159 kb on + strand, within EX28DRAFT_1207 at 1226.202 kb on + strand, within EX28DRAFT_1207 at 1226.202 kb on + strand, within EX28DRAFT_1207 at 1226.202 kb on + strand, within EX28DRAFT_1207 at 1226.203 kb on - strand, within EX28DRAFT_1207 at 1226.203 kb on - strand, within EX28DRAFT_1207 at 1226.205 kb on + strand at 1226.206 kb on - strand at 1226.208 kb on - strand at 1226.208 kb on - strand at 1226.309 kb on + strand at 1226.314 kb on + strand at 1226.315 kb on - strand at 1226.559 kb on + strand, within EX28DRAFT_1208 at 1226.559 kb on + strand, within EX28DRAFT_1208 at 1226.559 kb on + strand, within EX28DRAFT_1208 at 1226.559 kb on + strand, within EX28DRAFT_1208 at 1226.560 kb on - strand, within EX28DRAFT_1208 at 1226.560 kb on - strand, within EX28DRAFT_1208 at 1226.667 kb on - strand, within EX28DRAFT_1208 at 1226.726 kb on + strand, within EX28DRAFT_1208 at 1226.801 kb on + strand, within EX28DRAFT_1208 at 1226.801 kb on + strand, within EX28DRAFT_1208 at 1226.802 kb on - strand, within EX28DRAFT_1208 at 1227.074 kb on + strand at 1227.075 kb on - strand at 1227.118 kb on - strand at 1227.120 kb on + strand at 1227.121 kb on - strand at 1227.121 kb on - strand at 1227.162 kb on - strand at 1227.180 kb on + strand at 1227.180 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media with m-arsenite 1 mM remove 1,225,100 - -1.8 1,225,190 - EX28DRAFT_1205 0.12 -3.9 1,225,190 - EX28DRAFT_1205 0.12 -0.8 1,225,196 - EX28DRAFT_1205 0.13 -1.0 1,225,200 + EX28DRAFT_1205 0.14 +0.2 1,225,232 + EX28DRAFT_1205 0.21 -0.1 1,225,232 + EX28DRAFT_1205 0.21 +0.7 1,225,300 + EX28DRAFT_1205 0.37 -0.1 1,225,300 + EX28DRAFT_1205 0.37 +0.3 1,225,301 - EX28DRAFT_1205 0.37 -1.9 1,225,301 - EX28DRAFT_1205 0.37 -2.9 1,225,301 - EX28DRAFT_1205 0.37 -2.6 1,225,303 + EX28DRAFT_1205 0.38 +1.1 1,225,304 - EX28DRAFT_1205 0.38 -3.9 1,225,304 - EX28DRAFT_1205 0.38 -1.1 1,225,304 - EX28DRAFT_1205 0.38 -0.2 1,225,389 + EX28DRAFT_1205 0.57 -0.2 1,225,426 - EX28DRAFT_1205 0.66 -1.5 1,225,479 + EX28DRAFT_1205 0.78 +0.8 1,225,507 - EX28DRAFT_1205 0.85 +0.4 1,225,587 + -1.2 1,225,608 + -0.7 1,225,609 - -1.3 1,225,754 + EX28DRAFT_1206 0.42 +0.5 1,225,762 + EX28DRAFT_1206 0.44 -0.1 1,225,763 - EX28DRAFT_1206 0.44 -0.6 1,225,828 + EX28DRAFT_1206 0.61 -0.3 1,225,828 + EX28DRAFT_1206 0.61 -0.8 1,225,913 - EX28DRAFT_1206 0.84 -1.9 1,225,945 + +0.2 1,225,952 - -2.0 1,225,968 + +1.2 1,225,969 - -3.8 1,225,972 + -1.1 1,225,973 - -0.5 1,225,973 - +1.7 1,225,985 + -0.8 1,226,100 - EX28DRAFT_1207 0.42 -1.5 1,226,100 - EX28DRAFT_1207 0.42 +1.0 1,226,159 + EX28DRAFT_1207 0.69 -1.3 1,226,202 + EX28DRAFT_1207 0.89 -1.5 1,226,202 + EX28DRAFT_1207 0.89 -5.1 1,226,202 + EX28DRAFT_1207 0.89 -2.8 1,226,203 - EX28DRAFT_1207 0.89 -0.8 1,226,203 - EX28DRAFT_1207 0.89 -1.8 1,226,205 + -1.1 1,226,206 - +0.6 1,226,208 - -0.8 1,226,208 - -0.7 1,226,309 + +0.3 1,226,314 + -0.5 1,226,315 - -0.6 1,226,559 + EX28DRAFT_1208 0.33 -1.2 1,226,559 + EX28DRAFT_1208 0.33 -3.1 1,226,559 + EX28DRAFT_1208 0.33 -1.7 1,226,559 + EX28DRAFT_1208 0.33 +0.3 1,226,560 - EX28DRAFT_1208 0.33 -0.6 1,226,560 - EX28DRAFT_1208 0.33 -4.4 1,226,667 - EX28DRAFT_1208 0.45 -1.2 1,226,726 + EX28DRAFT_1208 0.52 -1.8 1,226,801 + EX28DRAFT_1208 0.60 +0.4 1,226,801 + EX28DRAFT_1208 0.60 -0.1 1,226,802 - EX28DRAFT_1208 0.60 -0.6 1,227,074 + +0.2 1,227,075 - -1.6 1,227,118 - -2.4 1,227,120 + -0.3 1,227,121 - -2.5 1,227,121 - -3.7 1,227,162 - -0.1 1,227,180 + +0.8 1,227,180 + +0.2
Or see this region's nucleotide sequence