Experiment: MS_media with m-arsenite 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0509 and EX28DRAFT_0510 are separated by 17 nucleotides EX28DRAFT_0510 and EX28DRAFT_0511 overlap by 4 nucleotides
EX28DRAFT_0509: EX28DRAFT_0509 - Membrane protein involved in the export of O-antigen and teichoic acid, at 501,660 to 503,138
_0509
EX28DRAFT_0510: EX28DRAFT_0510 - colanic acid biosynthesis pyruvyl transferase WcaK, at 503,156 to 504,436
_0510
EX28DRAFT_0511: EX28DRAFT_0511 - colanic acid biosynthesis glycosyltransferase WcaL, at 504,433 to 505,653
_0511
Position (kb) 503
504
505 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 502.329 kb on + strand, within EX28DRAFT_0509 at 502.329 kb on + strand, within EX28DRAFT_0509 at 502.329 kb on + strand, within EX28DRAFT_0509 at 502.330 kb on - strand, within EX28DRAFT_0509 at 502.330 kb on - strand, within EX28DRAFT_0509 at 502.408 kb on - strand, within EX28DRAFT_0509 at 502.408 kb on - strand, within EX28DRAFT_0509 at 502.855 kb on - strand, within EX28DRAFT_0509 at 502.899 kb on + strand, within EX28DRAFT_0509 at 502.900 kb on - strand, within EX28DRAFT_0509 at 503.137 kb on - strand at 503.140 kb on + strand at 503.167 kb on - strand at 503.182 kb on + strand at 503.182 kb on + strand at 503.183 kb on - strand at 503.183 kb on - strand at 503.224 kb on - strand at 503.224 kb on - strand at 503.237 kb on - strand at 503.507 kb on + strand, within EX28DRAFT_0510 at 503.585 kb on - strand, within EX28DRAFT_0510 at 503.669 kb on + strand, within EX28DRAFT_0510 at 503.819 kb on + strand, within EX28DRAFT_0510 at 503.928 kb on - strand, within EX28DRAFT_0510 at 504.018 kb on - strand, within EX28DRAFT_0510 at 504.018 kb on - strand, within EX28DRAFT_0510 at 504.018 kb on - strand, within EX28DRAFT_0510 at 504.080 kb on + strand, within EX28DRAFT_0510 at 504.274 kb on + strand, within EX28DRAFT_0510 at 504.274 kb on + strand, within EX28DRAFT_0510 at 504.276 kb on + strand, within EX28DRAFT_0510 at 504.276 kb on + strand, within EX28DRAFT_0510 at 504.276 kb on + strand, within EX28DRAFT_0510 at 504.276 kb on + strand, within EX28DRAFT_0510 at 504.276 kb on + strand, within EX28DRAFT_0510 at 504.276 kb on + strand, within EX28DRAFT_0510 at 504.276 kb on + strand, within EX28DRAFT_0510 at 504.277 kb on - strand, within EX28DRAFT_0510 at 504.277 kb on - strand, within EX28DRAFT_0510 at 504.277 kb on - strand, within EX28DRAFT_0510 at 504.277 kb on - strand, within EX28DRAFT_0510 at 504.277 kb on - strand, within EX28DRAFT_0510 at 504.277 kb on - strand, within EX28DRAFT_0510 at 504.408 kb on + strand at 504.577 kb on + strand, within EX28DRAFT_0511 at 504.578 kb on - strand, within EX28DRAFT_0511 at 504.935 kb on - strand, within EX28DRAFT_0511 at 505.428 kb on - strand, within EX28DRAFT_0511
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media with m-arsenite 1 mM remove 502,329 + EX28DRAFT_0509 0.45 -0.9 502,329 + EX28DRAFT_0509 0.45 +0.3 502,329 + EX28DRAFT_0509 0.45 -1.2 502,330 - EX28DRAFT_0509 0.45 -0.3 502,330 - EX28DRAFT_0509 0.45 +0.2 502,408 - EX28DRAFT_0509 0.51 +0.1 502,408 - EX28DRAFT_0509 0.51 -0.4 502,855 - EX28DRAFT_0509 0.81 -0.1 502,899 + EX28DRAFT_0509 0.84 -1.4 502,900 - EX28DRAFT_0509 0.84 +1.5 503,137 - +0.9 503,140 + -0.0 503,167 - +0.8 503,182 + +0.2 503,182 + -0.0 503,183 - +0.6 503,183 - -0.5 503,224 - -0.2 503,224 - -1.9 503,237 - +0.6 503,507 + EX28DRAFT_0510 0.27 -0.0 503,585 - EX28DRAFT_0510 0.33 +0.4 503,669 + EX28DRAFT_0510 0.40 -0.5 503,819 + EX28DRAFT_0510 0.52 -0.2 503,928 - EX28DRAFT_0510 0.60 -0.1 504,018 - EX28DRAFT_0510 0.67 -0.1 504,018 - EX28DRAFT_0510 0.67 +0.3 504,018 - EX28DRAFT_0510 0.67 -0.3 504,080 + EX28DRAFT_0510 0.72 +0.3 504,274 + EX28DRAFT_0510 0.87 -0.3 504,274 + EX28DRAFT_0510 0.87 -0.4 504,276 + EX28DRAFT_0510 0.87 -1.4 504,276 + EX28DRAFT_0510 0.87 +0.2 504,276 + EX28DRAFT_0510 0.87 +0.5 504,276 + EX28DRAFT_0510 0.87 +0.3 504,276 + EX28DRAFT_0510 0.87 +0.1 504,276 + EX28DRAFT_0510 0.87 +0.9 504,276 + EX28DRAFT_0510 0.87 -0.1 504,277 - EX28DRAFT_0510 0.88 +0.2 504,277 - EX28DRAFT_0510 0.88 -0.9 504,277 - EX28DRAFT_0510 0.88 +0.1 504,277 - EX28DRAFT_0510 0.88 -0.2 504,277 - EX28DRAFT_0510 0.88 -2.1 504,277 - EX28DRAFT_0510 0.88 -0.2 504,408 + +1.5 504,577 + EX28DRAFT_0511 0.12 +0.4 504,578 - EX28DRAFT_0511 0.12 +1.0 504,935 - EX28DRAFT_0511 0.41 +0.1 505,428 - EX28DRAFT_0511 0.81 -2.8
Or see this region's nucleotide sequence