Experiment: MS_media with m-arsenite 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0499 and EX28DRAFT_0500 are separated by 16 nucleotides EX28DRAFT_0500 and EX28DRAFT_0501 are separated by 15 nucleotides EX28DRAFT_0501 and EX28DRAFT_0502 are separated by 24 nucleotides
EX28DRAFT_0499: EX28DRAFT_0499 - putative colanic acid polymerase WcaD, at 490,894 to 492,114
_0499
EX28DRAFT_0500: EX28DRAFT_0500 - colanic acid biosynthesis glycosyl transferase WcaE, at 492,131 to 492,877
_0500
EX28DRAFT_0501: EX28DRAFT_0501 - colanic acid biosynthesis acetyltransferase WcaF, at 492,893 to 493,441
_0501
EX28DRAFT_0502: EX28DRAFT_0502 - GDP-mannose 4,6-dehydratase, at 493,466 to 494,587
_0502
Position (kb) 492
493 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 491.272 kb on - strand, within EX28DRAFT_0499 at 491.790 kb on - strand, within EX28DRAFT_0499 at 491.790 kb on - strand, within EX28DRAFT_0499 at 491.815 kb on + strand, within EX28DRAFT_0499 at 492.103 kb on - strand at 492.103 kb on - strand at 492.130 kb on + strand at 492.181 kb on - strand at 492.332 kb on - strand, within EX28DRAFT_0500 at 492.332 kb on - strand, within EX28DRAFT_0500 at 492.341 kb on + strand, within EX28DRAFT_0500 at 492.342 kb on - strand, within EX28DRAFT_0500 at 492.342 kb on - strand, within EX28DRAFT_0500 at 492.473 kb on + strand, within EX28DRAFT_0500 at 492.474 kb on - strand, within EX28DRAFT_0500 at 492.474 kb on - strand, within EX28DRAFT_0500 at 492.542 kb on + strand, within EX28DRAFT_0500 at 492.543 kb on - strand, within EX28DRAFT_0500 at 492.544 kb on + strand, within EX28DRAFT_0500 at 492.549 kb on - strand, within EX28DRAFT_0500 at 492.629 kb on - strand, within EX28DRAFT_0500 at 493.152 kb on - strand, within EX28DRAFT_0501 at 493.385 kb on - strand, within EX28DRAFT_0501 at 493.439 kb on + strand at 493.451 kb on + strand at 493.461 kb on + strand at 493.461 kb on + strand at 493.461 kb on + strand at 493.461 kb on + strand at 493.461 kb on + strand at 493.552 kb on - strand at 493.562 kb on - strand at 493.603 kb on + strand, within EX28DRAFT_0502 at 493.603 kb on + strand, within EX28DRAFT_0502 at 493.603 kb on + strand, within EX28DRAFT_0502 at 493.603 kb on + strand, within EX28DRAFT_0502 at 493.604 kb on - strand, within EX28DRAFT_0502 at 493.699 kb on - strand, within EX28DRAFT_0502 at 493.699 kb on - strand, within EX28DRAFT_0502
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media with m-arsenite 1 mM remove 491,272 - EX28DRAFT_0499 0.31 -0.1 491,790 - EX28DRAFT_0499 0.73 -1.3 491,790 - EX28DRAFT_0499 0.73 -0.9 491,815 + EX28DRAFT_0499 0.75 +0.9 492,103 - -0.3 492,103 - -2.0 492,130 + -0.1 492,181 - -0.9 492,332 - EX28DRAFT_0500 0.27 -0.5 492,332 - EX28DRAFT_0500 0.27 -0.0 492,341 + EX28DRAFT_0500 0.28 -0.2 492,342 - EX28DRAFT_0500 0.28 -0.8 492,342 - EX28DRAFT_0500 0.28 -0.6 492,473 + EX28DRAFT_0500 0.46 -0.3 492,474 - EX28DRAFT_0500 0.46 +0.6 492,474 - EX28DRAFT_0500 0.46 -0.5 492,542 + EX28DRAFT_0500 0.55 -1.5 492,543 - EX28DRAFT_0500 0.55 -1.2 492,544 + EX28DRAFT_0500 0.55 -1.5 492,549 - EX28DRAFT_0500 0.56 +0.9 492,629 - EX28DRAFT_0500 0.67 +0.1 493,152 - EX28DRAFT_0501 0.47 +0.8 493,385 - EX28DRAFT_0501 0.90 -1.0 493,439 + +0.6 493,451 + -3.1 493,461 + -0.0 493,461 + -3.3 493,461 + -1.3 493,461 + -1.2 493,461 + +1.2 493,552 - -2.6 493,562 - -0.3 493,603 + EX28DRAFT_0502 0.12 -1.3 493,603 + EX28DRAFT_0502 0.12 +0.2 493,603 + EX28DRAFT_0502 0.12 -0.4 493,603 + EX28DRAFT_0502 0.12 -1.3 493,604 - EX28DRAFT_0502 0.12 -0.2 493,699 - EX28DRAFT_0502 0.21 +0.3 493,699 - EX28DRAFT_0502 0.21 -1.6
Or see this region's nucleotide sequence