Experiment: MS_media with m-arsenite 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0271 and EX28DRAFT_0272 are separated by 224 nucleotides EX28DRAFT_0272 and EX28DRAFT_0273 are separated by 46 nucleotides EX28DRAFT_0273 and EX28DRAFT_0274 are separated by 145 nucleotides
EX28DRAFT_0271: EX28DRAFT_0271 - fatty oxidation complex, alpha subunit FadJ, at 232,605 to 234,752
_0271
EX28DRAFT_0272: EX28DRAFT_0272 - phosphohistidine phosphatase, SixA, at 234,977 to 235,447
_0272
EX28DRAFT_0273: EX28DRAFT_0273 - Uncharacterized protein conserved in bacteria, at 235,494 to 236,045
_0273
EX28DRAFT_0274: EX28DRAFT_0274 - protein-(glutamine-N5) methyltransferase, ribosomal protein L3-specific, at 236,191 to 237,123
_0274
Position (kb) 234
235
236 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 233.982 kb on - strand, within EX28DRAFT_0271 at 234.084 kb on + strand, within EX28DRAFT_0271 at 234.084 kb on + strand, within EX28DRAFT_0271 at 234.084 kb on + strand, within EX28DRAFT_0271 at 234.109 kb on - strand, within EX28DRAFT_0271 at 234.109 kb on - strand, within EX28DRAFT_0271 at 234.109 kb on - strand, within EX28DRAFT_0271 at 234.246 kb on - strand, within EX28DRAFT_0271 at 234.406 kb on + strand, within EX28DRAFT_0271 at 234.407 kb on - strand, within EX28DRAFT_0271 at 234.409 kb on - strand, within EX28DRAFT_0271 at 234.507 kb on - strand, within EX28DRAFT_0271 at 234.576 kb on + strand at 234.632 kb on + strand at 234.671 kb on + strand at 234.688 kb on + strand at 234.805 kb on - strand at 234.819 kb on + strand at 234.819 kb on + strand at 234.821 kb on + strand at 234.825 kb on + strand at 234.905 kb on + strand at 234.973 kb on + strand at 235.021 kb on - strand at 235.159 kb on + strand, within EX28DRAFT_0272 at 235.233 kb on - strand, within EX28DRAFT_0272 at 235.562 kb on - strand, within EX28DRAFT_0273 at 235.631 kb on + strand, within EX28DRAFT_0273 at 235.637 kb on + strand, within EX28DRAFT_0273 at 235.821 kb on + strand, within EX28DRAFT_0273 at 235.822 kb on - strand, within EX28DRAFT_0273 at 235.825 kb on + strand, within EX28DRAFT_0273 at 235.825 kb on + strand, within EX28DRAFT_0273 at 235.825 kb on + strand, within EX28DRAFT_0273 at 235.826 kb on - strand, within EX28DRAFT_0273 at 235.826 kb on - strand, within EX28DRAFT_0273 at 235.920 kb on + strand, within EX28DRAFT_0273 at 235.920 kb on + strand, within EX28DRAFT_0273 at 236.152 kb on - strand at 236.197 kb on - strand at 236.201 kb on + strand at 236.233 kb on - strand at 236.237 kb on + strand at 236.238 kb on - strand at 236.284 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media with m-arsenite 1 mM remove 233,982 - EX28DRAFT_0271 0.64 -1.2 234,084 + EX28DRAFT_0271 0.69 +0.9 234,084 + EX28DRAFT_0271 0.69 +1.0 234,084 + EX28DRAFT_0271 0.69 +1.4 234,109 - EX28DRAFT_0271 0.70 -0.4 234,109 - EX28DRAFT_0271 0.70 +0.9 234,109 - EX28DRAFT_0271 0.70 +1.0 234,246 - EX28DRAFT_0271 0.76 +0.3 234,406 + EX28DRAFT_0271 0.84 +0.1 234,407 - EX28DRAFT_0271 0.84 -0.7 234,409 - EX28DRAFT_0271 0.84 +0.8 234,507 - EX28DRAFT_0271 0.89 -1.1 234,576 + +0.8 234,632 + +0.8 234,671 + +0.5 234,688 + -0.8 234,805 - +0.2 234,819 + +1.0 234,819 + -0.5 234,821 + -0.1 234,825 + -0.8 234,905 + -1.3 234,973 + +0.1 235,021 - -3.1 235,159 + EX28DRAFT_0272 0.39 -3.5 235,233 - EX28DRAFT_0272 0.54 -3.6 235,562 - EX28DRAFT_0273 0.12 -1.2 235,631 + EX28DRAFT_0273 0.25 -0.1 235,637 + EX28DRAFT_0273 0.26 -0.7 235,821 + EX28DRAFT_0273 0.59 +0.4 235,822 - EX28DRAFT_0273 0.59 -0.7 235,825 + EX28DRAFT_0273 0.60 -1.3 235,825 + EX28DRAFT_0273 0.60 +0.9 235,825 + EX28DRAFT_0273 0.60 -1.5 235,826 - EX28DRAFT_0273 0.60 -0.4 235,826 - EX28DRAFT_0273 0.60 -1.7 235,920 + EX28DRAFT_0273 0.77 -0.2 235,920 + EX28DRAFT_0273 0.77 -1.5 236,152 - +2.1 236,197 - -0.0 236,201 + -0.0 236,233 - +0.5 236,237 + -1.2 236,238 - +0.8 236,284 - +0.2
Or see this region's nucleotide sequence