Experiment: MS_media with m-arsenite 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0212 and EX28DRAFT_0213 are separated by 44 nucleotides EX28DRAFT_0213 and EX28DRAFT_0214 are separated by 317 nucleotides EX28DRAFT_0214 and EX28DRAFT_0215 are separated by 163 nucleotides
EX28DRAFT_0212: EX28DRAFT_0212 - phosphoenolpyruvate-protein phosphotransferase, at 165,818 to 167,545
_0212
EX28DRAFT_0213: EX28DRAFT_0213 - Phosphotransferase System HPr (HPr) Family, at 167,590 to 167,847
_0213
EX28DRAFT_0214: EX28DRAFT_0214 - cysteine synthase A, at 168,165 to 169,136
_0214
EX28DRAFT_0215: EX28DRAFT_0215 - Uncharacterized protein involved in cysteine biosynthesis, at 169,300 to 170,061
_0215
Position (kb) 168
169
170 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1
2 at 167.864 kb on - strand at 168.573 kb on - strand, within EX28DRAFT_0214 at 168.575 kb on - strand, within EX28DRAFT_0214 at 168.621 kb on - strand, within EX28DRAFT_0214 at 168.621 kb on - strand, within EX28DRAFT_0214 at 168.724 kb on + strand, within EX28DRAFT_0214 at 168.834 kb on - strand, within EX28DRAFT_0214 at 169.094 kb on + strand at 169.095 kb on - strand at 169.154 kb on - strand at 169.154 kb on - strand at 169.179 kb on + strand at 169.180 kb on - strand at 169.204 kb on + strand at 169.204 kb on + strand at 169.205 kb on - strand at 169.218 kb on - strand at 169.218 kb on - strand at 169.221 kb on + strand at 169.221 kb on + strand at 169.221 kb on + strand at 169.221 kb on + strand at 169.221 kb on + strand at 169.222 kb on - strand at 169.222 kb on - strand at 169.224 kb on - strand at 169.239 kb on - strand at 169.287 kb on - strand at 169.287 kb on - strand at 169.592 kb on + strand, within EX28DRAFT_0215 at 169.594 kb on + strand, within EX28DRAFT_0215 at 169.841 kb on + strand, within EX28DRAFT_0215 at 169.842 kb on - strand, within EX28DRAFT_0215 at 169.871 kb on + strand, within EX28DRAFT_0215 at 169.941 kb on + strand, within EX28DRAFT_0215 at 169.941 kb on + strand, within EX28DRAFT_0215 at 169.954 kb on + strand, within EX28DRAFT_0215 at 170.072 kb on + strand at 170.107 kb on + strand at 170.108 kb on - strand at 170.109 kb on + strand at 170.109 kb on + strand at 170.109 kb on + strand at 170.109 kb on + strand at 170.110 kb on - strand at 170.110 kb on - strand at 170.121 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media with m-arsenite 1 mM remove 167,864 - -0.5 168,573 - EX28DRAFT_0214 0.42 -4.0 168,575 - EX28DRAFT_0214 0.42 -4.4 168,621 - EX28DRAFT_0214 0.47 -3.9 168,621 - EX28DRAFT_0214 0.47 -3.3 168,724 + EX28DRAFT_0214 0.58 -5.2 168,834 - EX28DRAFT_0214 0.69 -4.0 169,094 + -3.9 169,095 - -1.0 169,154 - -3.0 169,154 - -2.4 169,179 + -1.6 169,180 - -4.5 169,204 + -1.1 169,204 + -2.9 169,205 - -3.1 169,218 - +0.7 169,218 - -5.5 169,221 + -2.4 169,221 + -6.1 169,221 + -3.9 169,221 + -0.6 169,221 + -0.8 169,222 - -1.2 169,222 - -2.0 169,224 - +0.7 169,239 - -0.5 169,287 - +0.0 169,287 - +0.1 169,592 + EX28DRAFT_0215 0.38 +1.5 169,594 + EX28DRAFT_0215 0.39 +0.1 169,841 + EX28DRAFT_0215 0.71 +1.6 169,842 - EX28DRAFT_0215 0.71 +0.1 169,871 + EX28DRAFT_0215 0.75 -0.1 169,941 + EX28DRAFT_0215 0.84 +0.4 169,941 + EX28DRAFT_0215 0.84 +0.2 169,954 + EX28DRAFT_0215 0.86 +2.5 170,072 + -4.5 170,107 + +0.3 170,108 - +1.1 170,109 + +1.0 170,109 + +0.4 170,109 + +1.1 170,109 + -0.1 170,110 - +0.1 170,110 - -0.1 170,121 - -0.9
Or see this region's nucleotide sequence