Experiment: MS_media with m-arsenite 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0159 and EX28DRAFT_0160 are separated by 11 nucleotides EX28DRAFT_0160 and EX28DRAFT_0161 are separated by 50 nucleotides EX28DRAFT_0161 and EX28DRAFT_0162 are separated by 97 nucleotides EX28DRAFT_0162 and EX28DRAFT_0163 overlap by 4 nucleotides
EX28DRAFT_0159: EX28DRAFT_0159 - Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily), at 105,974 to 106,717
_0159
EX28DRAFT_0160: EX28DRAFT_0160 - hypothetical protein, at 106,729 to 107,196
_0160
EX28DRAFT_0161: EX28DRAFT_0161 - Cyanate permease, at 107,247 to 108,476
_0161
EX28DRAFT_0162: EX28DRAFT_0162 - Transcriptional regulator, at 108,574 to 109,464
_0162
EX28DRAFT_0163: EX28DRAFT_0163 - Protein of unknown function (DUF2633), at 109,461 to 109,631
_0163
Position (kb) 107
108
109 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 106.289 kb on + strand, within EX28DRAFT_0159 at 106.289 kb on + strand, within EX28DRAFT_0159 at 106.289 kb on + strand, within EX28DRAFT_0159 at 106.289 kb on + strand, within EX28DRAFT_0159 at 106.290 kb on - strand, within EX28DRAFT_0159 at 106.290 kb on - strand, within EX28DRAFT_0159 at 106.310 kb on - strand, within EX28DRAFT_0159 at 106.442 kb on + strand, within EX28DRAFT_0159 at 106.597 kb on + strand, within EX28DRAFT_0159 at 106.651 kb on + strand at 106.701 kb on - strand at 106.816 kb on - strand, within EX28DRAFT_0160 at 106.816 kb on - strand, within EX28DRAFT_0160 at 107.248 kb on + strand at 107.261 kb on + strand at 107.322 kb on + strand at 107.322 kb on + strand at 107.426 kb on + strand, within EX28DRAFT_0161 at 107.506 kb on + strand, within EX28DRAFT_0161 at 107.507 kb on - strand, within EX28DRAFT_0161 at 107.609 kb on + strand, within EX28DRAFT_0161 at 107.609 kb on + strand, within EX28DRAFT_0161 at 107.609 kb on + strand, within EX28DRAFT_0161 at 107.609 kb on + strand, within EX28DRAFT_0161 at 107.783 kb on + strand, within EX28DRAFT_0161 at 108.102 kb on + strand, within EX28DRAFT_0161 at 108.103 kb on - strand, within EX28DRAFT_0161 at 108.173 kb on - strand, within EX28DRAFT_0161 at 108.335 kb on - strand, within EX28DRAFT_0161 at 108.398 kb on - strand at 108.428 kb on + strand at 108.428 kb on + strand at 108.428 kb on + strand at 108.492 kb on + strand at 108.524 kb on - strand at 108.549 kb on - strand at 108.557 kb on + strand at 108.559 kb on + strand at 108.689 kb on + strand, within EX28DRAFT_0162 at 108.861 kb on + strand, within EX28DRAFT_0162 at 108.861 kb on + strand, within EX28DRAFT_0162 at 108.862 kb on - strand, within EX28DRAFT_0162 at 108.862 kb on - strand, within EX28DRAFT_0162 at 109.084 kb on - strand, within EX28DRAFT_0162 at 109.293 kb on + strand, within EX28DRAFT_0162 at 109.293 kb on + strand, within EX28DRAFT_0162 at 109.293 kb on + strand, within EX28DRAFT_0162 at 109.294 kb on - strand, within EX28DRAFT_0162 at 109.388 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media with m-arsenite 1 mM remove 106,289 + EX28DRAFT_0159 0.42 +0.7 106,289 + EX28DRAFT_0159 0.42 +0.1 106,289 + EX28DRAFT_0159 0.42 +0.9 106,289 + EX28DRAFT_0159 0.42 +1.0 106,290 - EX28DRAFT_0159 0.42 +1.3 106,290 - EX28DRAFT_0159 0.42 +0.6 106,310 - EX28DRAFT_0159 0.45 -0.6 106,442 + EX28DRAFT_0159 0.63 -0.5 106,597 + EX28DRAFT_0159 0.84 +0.9 106,651 + +0.0 106,701 - +0.0 106,816 - EX28DRAFT_0160 0.19 -0.3 106,816 - EX28DRAFT_0160 0.19 +2.1 107,248 + -1.2 107,261 + -0.4 107,322 + +2.3 107,322 + +1.6 107,426 + EX28DRAFT_0161 0.15 +0.4 107,506 + EX28DRAFT_0161 0.21 -0.4 107,507 - EX28DRAFT_0161 0.21 -0.9 107,609 + EX28DRAFT_0161 0.29 +0.7 107,609 + EX28DRAFT_0161 0.29 -1.4 107,609 + EX28DRAFT_0161 0.29 +2.1 107,609 + EX28DRAFT_0161 0.29 +0.2 107,783 + EX28DRAFT_0161 0.44 -0.6 108,102 + EX28DRAFT_0161 0.70 +0.4 108,103 - EX28DRAFT_0161 0.70 +0.4 108,173 - EX28DRAFT_0161 0.75 +0.4 108,335 - EX28DRAFT_0161 0.88 -2.2 108,398 - +1.7 108,428 + -0.2 108,428 + -3.7 108,428 + +0.2 108,492 + -4.6 108,524 - +0.1 108,549 - -0.0 108,557 + -0.3 108,559 + +1.6 108,689 + EX28DRAFT_0162 0.13 -0.2 108,861 + EX28DRAFT_0162 0.32 -0.4 108,861 + EX28DRAFT_0162 0.32 -1.0 108,862 - EX28DRAFT_0162 0.32 -1.6 108,862 - EX28DRAFT_0162 0.32 -1.6 109,084 - EX28DRAFT_0162 0.57 -2.0 109,293 + EX28DRAFT_0162 0.81 -0.6 109,293 + EX28DRAFT_0162 0.81 +0.5 109,293 + EX28DRAFT_0162 0.81 +0.1 109,294 - EX28DRAFT_0162 0.81 -1.9 109,388 + -1.0
Or see this region's nucleotide sequence