Experiment: MS_media with m-arsenite 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0066 and EX28DRAFT_0067 are separated by 40 nucleotides EX28DRAFT_0067 and EX28DRAFT_0068 are separated by 313 nucleotides EX28DRAFT_0068 and EX28DRAFT_0069 are separated by 50 nucleotides
EX28DRAFT_0066: EX28DRAFT_0066 - rRNA methylases, at 7,739 to 8,869
_0066
EX28DRAFT_0067: EX28DRAFT_0067 - Uracil-DNA glycosylase (EC 3.2.2.-), at 8,910 to 9,599
_0067
EX28DRAFT_0068: EX28DRAFT_0068 - autonomous glycyl radical cofactor GrcA, at 9,913 to 10,296
_0068
EX28DRAFT_0069: EX28DRAFT_0069 - Superfamily II DNA and RNA helicases, at 10,347 to 11,675
_0069
Position (kb)
8
9
10 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 8.210 kb on - strand, within EX28DRAFT_0066 at 8.321 kb on - strand, within EX28DRAFT_0066 at 8.555 kb on - strand, within EX28DRAFT_0066 at 8.580 kb on + strand, within EX28DRAFT_0066 at 8.629 kb on + strand, within EX28DRAFT_0066 at 8.715 kb on + strand, within EX28DRAFT_0066 at 8.716 kb on - strand, within EX28DRAFT_0066 at 8.867 kb on + strand at 8.867 kb on + strand at 8.868 kb on - strand at 8.876 kb on + strand at 8.912 kb on - strand at 8.928 kb on + strand at 8.931 kb on + strand at 8.931 kb on + strand at 9.120 kb on + strand, within EX28DRAFT_0067 at 9.146 kb on + strand, within EX28DRAFT_0067 at 9.230 kb on + strand, within EX28DRAFT_0067 at 9.321 kb on - strand, within EX28DRAFT_0067 at 9.322 kb on + strand, within EX28DRAFT_0067 at 9.322 kb on + strand, within EX28DRAFT_0067 at 9.322 kb on + strand, within EX28DRAFT_0067 at 9.322 kb on + strand, within EX28DRAFT_0067 at 9.323 kb on - strand, within EX28DRAFT_0067 at 9.323 kb on - strand, within EX28DRAFT_0067 at 9.323 kb on - strand, within EX28DRAFT_0067 at 9.323 kb on - strand, within EX28DRAFT_0067 at 9.323 kb on - strand, within EX28DRAFT_0067 at 9.539 kb on + strand at 9.540 kb on - strand at 9.544 kb on + strand at 9.545 kb on - strand at 9.631 kb on - strand at 9.635 kb on + strand at 9.830 kb on + strand at 9.830 kb on + strand at 9.831 kb on - strand at 9.832 kb on + strand at 9.832 kb on + strand at 9.832 kb on + strand at 9.832 kb on + strand at 9.832 kb on + strand at 9.832 kb on + strand at 9.832 kb on + strand at 9.832 kb on + strand at 9.833 kb on - strand at 9.833 kb on - strand at 9.833 kb on - strand at 9.834 kb on + strand at 9.834 kb on + strand at 9.834 kb on + strand at 9.835 kb on - strand at 9.836 kb on + strand at 9.889 kb on + strand at 9.889 kb on + strand at 9.890 kb on - strand at 9.890 kb on - strand at 9.895 kb on - strand at 9.918 kb on + strand at 9.919 kb on - strand at 10.072 kb on + strand, within EX28DRAFT_0068 at 10.137 kb on - strand, within EX28DRAFT_0068 at 10.275 kb on + strand at 10.403 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media with m-arsenite 1 mM remove 8,210 - EX28DRAFT_0066 0.42 +0.7 8,321 - EX28DRAFT_0066 0.51 -1.0 8,555 - EX28DRAFT_0066 0.72 +1.3 8,580 + EX28DRAFT_0066 0.74 +0.3 8,629 + EX28DRAFT_0066 0.79 +0.0 8,715 + EX28DRAFT_0066 0.86 -0.1 8,716 - EX28DRAFT_0066 0.86 -0.1 8,867 + +0.3 8,867 + +0.1 8,868 - +0.2 8,876 + +0.3 8,912 - -1.5 8,928 + +0.1 8,931 + -0.1 8,931 + -1.2 9,120 + EX28DRAFT_0067 0.30 -4.1 9,146 + EX28DRAFT_0067 0.34 +0.0 9,230 + EX28DRAFT_0067 0.46 -2.4 9,321 - EX28DRAFT_0067 0.60 -0.2 9,322 + EX28DRAFT_0067 0.60 +0.5 9,322 + EX28DRAFT_0067 0.60 +0.6 9,322 + EX28DRAFT_0067 0.60 +0.9 9,322 + EX28DRAFT_0067 0.60 -0.2 9,323 - EX28DRAFT_0067 0.60 +0.7 9,323 - EX28DRAFT_0067 0.60 +1.1 9,323 - EX28DRAFT_0067 0.60 -0.5 9,323 - EX28DRAFT_0067 0.60 -0.0 9,323 - EX28DRAFT_0067 0.60 -0.2 9,539 + -0.4 9,540 - +1.0 9,544 + -1.6 9,545 - +0.9 9,631 - +0.5 9,635 + -3.0 9,830 + +0.3 9,830 + +0.2 9,831 - +0.3 9,832 + -1.1 9,832 + +0.0 9,832 + -0.3 9,832 + +0.2 9,832 + -0.5 9,832 + -2.3 9,832 + -0.4 9,832 + +0.1 9,833 - +0.6 9,833 - -1.5 9,833 - +0.8 9,834 + +0.3 9,834 + -0.5 9,834 + +0.0 9,835 - +0.5 9,836 + +2.1 9,889 + -1.0 9,889 + +1.6 9,890 - +1.0 9,890 - -0.7 9,895 - +0.4 9,918 + +1.3 9,919 - +1.5 10,072 + EX28DRAFT_0068 0.41 -3.0 10,137 - EX28DRAFT_0068 0.58 -4.2 10,275 + -3.0 10,403 + -0.0
Or see this region's nucleotide sequence