Experiment: MS_media with m-arsenite 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0001 and EX28DRAFT_0002 are separated by 179 nucleotides EX28DRAFT_0002 and EX28DRAFT_0003 are separated by 72 nucleotides EX28DRAFT_0003 and EX28DRAFT_0004 overlap by 17 nucleotides EX28DRAFT_0004 and EX28DRAFT_0005 are separated by 341 nucleotides
EX28DRAFT_0001: EX28DRAFT_0001 - Elongation factor Tu domain 2/Elongation factor Tu C-terminal domain, at 1 to 504
_0001
EX28DRAFT_0002: EX28DRAFT_0002 - Bacterioferritin-associated ferredoxin, at 684 to 878
_0002
EX28DRAFT_0003: EX28DRAFT_0003 - bacterioferritin, at 951 to 1,427
_0003
EX28DRAFT_0004: EX28DRAFT_0004 - Type IV leader peptidase family, at 1,411 to 1,923
_0004
EX28DRAFT_0005: EX28DRAFT_0005 - SSU ribosomal protein S10P, at 2,265 to 2,576
_0005
Position (kb)
0
1
2 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 0.075 kb on + strand, within EX28DRAFT_0001 at 0.081 kb on + strand, within EX28DRAFT_0001 at 0.313 kb on + strand, within EX28DRAFT_0001 at 0.567 kb on + strand at 0.568 kb on - strand at 0.568 kb on - strand at 0.568 kb on - strand at 0.684 kb on - strand at 0.684 kb on - strand at 0.687 kb on + strand at 0.688 kb on - strand at 0.697 kb on + strand at 0.698 kb on - strand at 0.701 kb on + strand at 0.701 kb on + strand at 0.701 kb on + strand at 0.724 kb on + strand, within EX28DRAFT_0002 at 0.803 kb on + strand, within EX28DRAFT_0002 at 0.811 kb on + strand, within EX28DRAFT_0002 at 0.812 kb on - strand, within EX28DRAFT_0002 at 0.812 kb on - strand, within EX28DRAFT_0002 at 0.812 kb on - strand, within EX28DRAFT_0002 at 0.862 kb on - strand at 0.916 kb on + strand at 0.945 kb on + strand at 0.947 kb on + strand at 0.947 kb on + strand at 0.947 kb on + strand at 0.948 kb on - strand at 0.948 kb on - strand at 0.948 kb on - strand at 0.948 kb on - strand at 0.948 kb on - strand at 0.948 kb on - strand at 0.949 kb on + strand at 0.950 kb on - strand at 0.950 kb on - strand at 1.024 kb on - strand, within EX28DRAFT_0003 at 1.024 kb on - strand, within EX28DRAFT_0003 at 1.061 kb on + strand, within EX28DRAFT_0003 at 1.061 kb on + strand, within EX28DRAFT_0003 at 1.122 kb on + strand, within EX28DRAFT_0003 at 1.122 kb on + strand, within EX28DRAFT_0003 at 1.123 kb on - strand, within EX28DRAFT_0003 at 1.123 kb on - strand, within EX28DRAFT_0003 at 1.123 kb on - strand, within EX28DRAFT_0003 at 1.282 kb on + strand, within EX28DRAFT_0003 at 1.283 kb on - strand, within EX28DRAFT_0003 at 1.283 kb on - strand, within EX28DRAFT_0003 at 1.283 kb on - strand, within EX28DRAFT_0003 at 1.290 kb on + strand, within EX28DRAFT_0003 at 1.291 kb on - strand, within EX28DRAFT_0003 at 1.291 kb on - strand, within EX28DRAFT_0003 at 1.395 kb on + strand at 1.444 kb on + strand at 1.539 kb on + strand, within EX28DRAFT_0004 at 1.540 kb on - strand, within EX28DRAFT_0004 at 1.625 kb on - strand, within EX28DRAFT_0004 at 1.677 kb on - strand, within EX28DRAFT_0004 at 1.677 kb on - strand, within EX28DRAFT_0004 at 1.698 kb on - strand, within EX28DRAFT_0004 at 1.818 kb on + strand, within EX28DRAFT_0004 at 1.994 kb on + strand at 1.995 kb on - strand at 1.995 kb on - strand at 2.027 kb on + strand at 2.028 kb on - strand at 2.028 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media with m-arsenite 1 mM remove 75 + EX28DRAFT_0001 0.15 -0.8 81 + EX28DRAFT_0001 0.16 -0.3 313 + EX28DRAFT_0001 0.62 +0.7 567 + -0.2 568 - +2.5 568 - +2.5 568 - +0.1 684 - +0.2 684 - -0.1 687 + -0.4 688 - -0.5 697 + -0.7 698 - +1.6 701 + +0.6 701 + +2.3 701 + -0.4 724 + EX28DRAFT_0002 0.21 +0.5 803 + EX28DRAFT_0002 0.61 -0.7 811 + EX28DRAFT_0002 0.65 -0.4 812 - EX28DRAFT_0002 0.66 -0.1 812 - EX28DRAFT_0002 0.66 -3.8 812 - EX28DRAFT_0002 0.66 -0.3 862 - +0.7 916 + +0.3 945 + +0.6 947 + +0.4 947 + +0.5 947 + +1.1 948 - +0.4 948 - +0.5 948 - +0.4 948 - +1.0 948 - +0.8 948 - +0.1 949 + +0.8 950 - +1.2 950 - +0.5 1,024 - EX28DRAFT_0003 0.15 +0.8 1,024 - EX28DRAFT_0003 0.15 +0.9 1,061 + EX28DRAFT_0003 0.23 +0.4 1,061 + EX28DRAFT_0003 0.23 +0.1 1,122 + EX28DRAFT_0003 0.36 +0.5 1,122 + EX28DRAFT_0003 0.36 +0.0 1,123 - EX28DRAFT_0003 0.36 +1.6 1,123 - EX28DRAFT_0003 0.36 +0.9 1,123 - EX28DRAFT_0003 0.36 -1.6 1,282 + EX28DRAFT_0003 0.69 +1.8 1,283 - EX28DRAFT_0003 0.70 -0.0 1,283 - EX28DRAFT_0003 0.70 +0.2 1,283 - EX28DRAFT_0003 0.70 -0.2 1,290 + EX28DRAFT_0003 0.71 +0.7 1,291 - EX28DRAFT_0003 0.71 +1.0 1,291 - EX28DRAFT_0003 0.71 -1.4 1,395 + +2.3 1,444 + +0.5 1,539 + EX28DRAFT_0004 0.25 -1.9 1,540 - EX28DRAFT_0004 0.25 +0.8 1,625 - EX28DRAFT_0004 0.42 +0.4 1,677 - EX28DRAFT_0004 0.52 +0.3 1,677 - EX28DRAFT_0004 0.52 +1.2 1,698 - EX28DRAFT_0004 0.56 +1.0 1,818 + EX28DRAFT_0004 0.79 +0.5 1,994 + +1.5 1,995 - +1.1 1,995 - -0.2 2,027 + +0.4 2,028 - -0.2 2,028 - -3.1
Or see this region's nucleotide sequence