Experiment: MS_media with m-arsenite 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_4408 and EX28DRAFT_4409 are separated by 200 nucleotides EX28DRAFT_4409 and EX28DRAFT_4410 are separated by 48 nucleotides EX28DRAFT_4410 and EX28DRAFT_4411 are separated by 101 nucleotides EX28DRAFT_4411 and EX28DRAFT_4412 are separated by 2 nucleotides
EX28DRAFT_4408: EX28DRAFT_4408 - replication restart DNA helicase PriA, at 70,409 to 72,604
_4408
EX28DRAFT_4409: EX28DRAFT_4409 - ribosomal protein L31, at 72,805 to 73,017
_4409
EX28DRAFT_4410: EX28DRAFT_4410 - methionine repressor, MetJ, at 73,066 to 73,383
_4410
EX28DRAFT_4411: EX28DRAFT_4411 - cystathionine gamma-synthase (EC 2.5.1.48), at 73,485 to 74,820
_4411
EX28DRAFT_4412: EX28DRAFT_4412 - aspartate kinase (EC 2.7.2.4), at 74,823 to 77,255
_4412
Position (kb) 73
74
75 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1 at 72.635 kb on + strand at 72.636 kb on - strand at 72.638 kb on + strand at 72.638 kb on + strand at 72.639 kb on - strand at 72.640 kb on + strand at 72.641 kb on - strand at 72.641 kb on - strand at 72.641 kb on - strand at 72.647 kb on + strand at 72.648 kb on - strand at 72.650 kb on + strand at 73.141 kb on - strand, within EX28DRAFT_4410 at 73.360 kb on - strand at 73.558 kb on + strand at 73.588 kb on + strand at 73.613 kb on + strand at 73.668 kb on + strand, within EX28DRAFT_4411 at 73.681 kb on - strand, within EX28DRAFT_4411 at 73.756 kb on + strand, within EX28DRAFT_4411 at 73.757 kb on - strand, within EX28DRAFT_4411 at 73.757 kb on - strand, within EX28DRAFT_4411 at 73.757 kb on - strand, within EX28DRAFT_4411 at 73.757 kb on - strand, within EX28DRAFT_4411 at 73.757 kb on - strand, within EX28DRAFT_4411 at 73.815 kb on + strand, within EX28DRAFT_4411 at 73.871 kb on + strand, within EX28DRAFT_4411 at 73.872 kb on - strand, within EX28DRAFT_4411 at 73.872 kb on - strand, within EX28DRAFT_4411 at 73.960 kb on + strand, within EX28DRAFT_4411 at 73.972 kb on + strand, within EX28DRAFT_4411 at 74.166 kb on - strand, within EX28DRAFT_4411 at 74.166 kb on - strand, within EX28DRAFT_4411 at 74.172 kb on - strand, within EX28DRAFT_4411 at 74.181 kb on + strand, within EX28DRAFT_4411 at 74.181 kb on + strand, within EX28DRAFT_4411 at 74.181 kb on + strand, within EX28DRAFT_4411 at 74.182 kb on - strand, within EX28DRAFT_4411 at 74.182 kb on - strand, within EX28DRAFT_4411 at 74.249 kb on + strand, within EX28DRAFT_4411 at 74.250 kb on + strand, within EX28DRAFT_4411 at 74.250 kb on + strand, within EX28DRAFT_4411 at 74.250 kb on + strand, within EX28DRAFT_4411 at 74.251 kb on - strand, within EX28DRAFT_4411 at 74.254 kb on + strand, within EX28DRAFT_4411 at 74.341 kb on - strand, within EX28DRAFT_4411 at 74.374 kb on + strand, within EX28DRAFT_4411 at 74.374 kb on + strand, within EX28DRAFT_4411 at 74.375 kb on - strand, within EX28DRAFT_4411 at 74.375 kb on - strand, within EX28DRAFT_4411 at 74.486 kb on - strand, within EX28DRAFT_4411 at 74.558 kb on + strand, within EX28DRAFT_4411 at 74.558 kb on + strand, within EX28DRAFT_4411 at 74.623 kb on - strand, within EX28DRAFT_4411 at 74.640 kb on - strand, within EX28DRAFT_4411 at 74.692 kb on - strand at 74.743 kb on - strand at 74.775 kb on - strand at 74.882 kb on + strand at 75.187 kb on - strand, within EX28DRAFT_4412 at 75.187 kb on - strand, within EX28DRAFT_4412 at 75.383 kb on + strand, within EX28DRAFT_4412 at 75.383 kb on + strand, within EX28DRAFT_4412 at 75.401 kb on + strand, within EX28DRAFT_4412
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media with m-arsenite 1 mM remove 72,635 + -0.6 72,636 - -1.2 72,638 + +0.1 72,638 + +0.7 72,639 - +0.7 72,640 + +0.7 72,641 - -0.7 72,641 - -0.6 72,641 - -1.3 72,647 + -0.7 72,648 - -0.3 72,650 + -2.6 73,141 - EX28DRAFT_4410 0.24 -1.2 73,360 - -1.2 73,558 + -0.1 73,588 + -1.0 73,613 + -4.9 73,668 + EX28DRAFT_4411 0.14 -3.9 73,681 - EX28DRAFT_4411 0.15 +0.5 73,756 + EX28DRAFT_4411 0.20 -3.2 73,757 - EX28DRAFT_4411 0.20 -0.1 73,757 - EX28DRAFT_4411 0.20 -3.1 73,757 - EX28DRAFT_4411 0.20 -4.5 73,757 - EX28DRAFT_4411 0.20 -0.5 73,757 - EX28DRAFT_4411 0.20 +0.9 73,815 + EX28DRAFT_4411 0.25 -3.9 73,871 + EX28DRAFT_4411 0.29 -2.9 73,872 - EX28DRAFT_4411 0.29 -4.4 73,872 - EX28DRAFT_4411 0.29 -1.7 73,960 + EX28DRAFT_4411 0.36 -2.9 73,972 + EX28DRAFT_4411 0.36 -1.0 74,166 - EX28DRAFT_4411 0.51 -2.8 74,166 - EX28DRAFT_4411 0.51 -3.2 74,172 - EX28DRAFT_4411 0.51 +0.4 74,181 + EX28DRAFT_4411 0.52 -1.7 74,181 + EX28DRAFT_4411 0.52 -3.1 74,181 + EX28DRAFT_4411 0.52 -5.4 74,182 - EX28DRAFT_4411 0.52 -5.7 74,182 - EX28DRAFT_4411 0.52 -2.8 74,249 + EX28DRAFT_4411 0.57 -3.1 74,250 + EX28DRAFT_4411 0.57 -1.9 74,250 + EX28DRAFT_4411 0.57 -3.8 74,250 + EX28DRAFT_4411 0.57 -1.8 74,251 - EX28DRAFT_4411 0.57 -1.4 74,254 + EX28DRAFT_4411 0.58 -2.0 74,341 - EX28DRAFT_4411 0.64 -1.0 74,374 + EX28DRAFT_4411 0.67 -2.9 74,374 + EX28DRAFT_4411 0.67 -4.4 74,375 - EX28DRAFT_4411 0.67 -1.1 74,375 - EX28DRAFT_4411 0.67 -3.2 74,486 - EX28DRAFT_4411 0.75 -1.7 74,558 + EX28DRAFT_4411 0.80 -2.1 74,558 + EX28DRAFT_4411 0.80 -6.0 74,623 - EX28DRAFT_4411 0.85 -3.3 74,640 - EX28DRAFT_4411 0.86 -1.1 74,692 - -3.1 74,743 - -3.9 74,775 - -1.7 74,882 + +0.3 75,187 - EX28DRAFT_4412 0.15 +1.0 75,187 - EX28DRAFT_4412 0.15 +1.3 75,383 + EX28DRAFT_4412 0.23 +0.9 75,383 + EX28DRAFT_4412 0.23 +0.7 75,401 + EX28DRAFT_4412 0.24 -2.5
Or see this region's nucleotide sequence