Experiment: MS_media with m-arsenite 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_4295 and EX28DRAFT_4296 are separated by 251 nucleotides EX28DRAFT_4296 and EX28DRAFT_4297 overlap by 14 nucleotides EX28DRAFT_4297 and EX28DRAFT_4298 are separated by 2 nucleotides
EX28DRAFT_4295: EX28DRAFT_4295 - alternative sigma factor RpoH, at 98,851 to 99,708
_4295
EX28DRAFT_4296: EX28DRAFT_4296 - putative protein insertion permease FtsX, at 99,960 to 101,015
_4296
EX28DRAFT_4297: EX28DRAFT_4297 - cell division ATP-binding protein FtsE, at 101,002 to 101,673
_4297
EX28DRAFT_4298: EX28DRAFT_4298 - signal recognition particle-docking protein FtsY, at 101,676 to 103,136
_4298
Position (kb) 99
100
101
102 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 99.945 kb on + strand at 99.946 kb on - strand at 99.961 kb on + strand at 99.971 kb on + strand at 100.559 kb on + strand, within EX28DRAFT_4296 at 100.594 kb on + strand, within EX28DRAFT_4296 at 100.595 kb on - strand, within EX28DRAFT_4296 at 100.670 kb on + strand, within EX28DRAFT_4296 at 100.677 kb on - strand, within EX28DRAFT_4296 at 101.104 kb on + strand, within EX28DRAFT_4297 at 101.532 kb on + strand, within EX28DRAFT_4297 at 101.643 kb on - strand at 101.678 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media with m-arsenite 1 mM remove 99,945 + -0.7 99,946 - -0.2 99,961 + -1.6 99,971 + +1.3 100,559 + EX28DRAFT_4296 0.57 -2.4 100,594 + EX28DRAFT_4296 0.60 -2.9 100,595 - EX28DRAFT_4296 0.60 -1.4 100,670 + EX28DRAFT_4296 0.67 -0.4 100,677 - EX28DRAFT_4296 0.68 -2.7 101,104 + EX28DRAFT_4297 0.15 -1.4 101,532 + EX28DRAFT_4297 0.79 -2.5 101,643 - -1.8 101,678 - +0.9
Or see this region's nucleotide sequence