Experiment: MS_media with m-arsenite 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_4148 and EX28DRAFT_4149 are separated by 3 nucleotides EX28DRAFT_4149 and EX28DRAFT_4150 overlap by 11 nucleotides EX28DRAFT_4150 and EX28DRAFT_4151 overlap by 11 nucleotides EX28DRAFT_4151 and EX28DRAFT_4152 are separated by 286 nucleotides EX28DRAFT_4152 and EX28DRAFT_4153 are separated by 57 nucleotides
EX28DRAFT_4148: EX28DRAFT_4148 - Tfp pilus assembly protein PilN, at 130,547 to 131,041
_4148
EX28DRAFT_4149: EX28DRAFT_4149 - hypothetical protein, at 131,045 to 131,500
_4149
EX28DRAFT_4150: EX28DRAFT_4150 - Protein of unknown function (DUF2531), at 131,490 to 131,894
_4150
EX28DRAFT_4151: EX28DRAFT_4151 - type IV pilus secretin (or competence protein) PilQ, at 131,884 to 133,044
_4151
EX28DRAFT_4152: EX28DRAFT_4152 - shikimate kinase (EC 2.7.1.71), at 133,331 to 133,852
_4152
EX28DRAFT_4153: EX28DRAFT_4153 - 3-dehydroquinate synthase (EC 4.2.3.4), at 133,910 to 134,998
_4153
Position (kb)
131
132
133
134 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 131.027 kb on - strand at 131.059 kb on - strand at 131.459 kb on - strand at 131.533 kb on + strand, within EX28DRAFT_4150 at 131.533 kb on + strand, within EX28DRAFT_4150 at 131.647 kb on + strand, within EX28DRAFT_4150 at 131.801 kb on + strand, within EX28DRAFT_4150 at 131.904 kb on - strand at 132.182 kb on - strand, within EX28DRAFT_4151 at 132.364 kb on - strand, within EX28DRAFT_4151 at 132.364 kb on - strand, within EX28DRAFT_4151 at 132.364 kb on - strand, within EX28DRAFT_4151 at 132.780 kb on + strand, within EX28DRAFT_4151 at 132.842 kb on + strand, within EX28DRAFT_4151 at 132.842 kb on + strand, within EX28DRAFT_4151 at 133.005 kb on - strand at 133.015 kb on - strand at 133.015 kb on - strand at 133.018 kb on - strand at 133.111 kb on + strand at 133.112 kb on - strand at 133.117 kb on - strand at 133.117 kb on - strand at 133.117 kb on - strand at 133.117 kb on - strand at 133.117 kb on - strand at 133.117 kb on - strand at 133.145 kb on - strand at 133.151 kb on + strand at 133.151 kb on + strand at 133.152 kb on - strand at 133.152 kb on - strand at 133.152 kb on - strand at 133.159 kb on + strand at 133.181 kb on - strand at 133.237 kb on + strand at 133.238 kb on - strand at 133.244 kb on + strand at 133.244 kb on + strand at 133.315 kb on + strand at 133.318 kb on + strand at 133.349 kb on - strand at 133.488 kb on + strand, within EX28DRAFT_4152 at 133.634 kb on + strand, within EX28DRAFT_4152 at 133.828 kb on + strand at 133.862 kb on + strand at 133.864 kb on + strand at 133.864 kb on + strand at 133.865 kb on - strand at 133.865 kb on - strand at 133.866 kb on + strand at 133.866 kb on + strand at 133.866 kb on + strand at 133.867 kb on - strand at 133.891 kb on + strand at 133.927 kb on + strand at 133.943 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media with m-arsenite 1 mM remove 131,027 - -0.3 131,059 - +0.2 131,459 - -0.5 131,533 + EX28DRAFT_4150 0.11 +0.2 131,533 + EX28DRAFT_4150 0.11 -0.0 131,647 + EX28DRAFT_4150 0.39 -0.1 131,801 + EX28DRAFT_4150 0.77 -0.4 131,904 - +1.2 132,182 - EX28DRAFT_4151 0.26 +0.6 132,364 - EX28DRAFT_4151 0.41 -0.0 132,364 - EX28DRAFT_4151 0.41 -0.5 132,364 - EX28DRAFT_4151 0.41 +1.4 132,780 + EX28DRAFT_4151 0.77 -0.6 132,842 + EX28DRAFT_4151 0.83 -0.1 132,842 + EX28DRAFT_4151 0.83 +0.1 133,005 - +1.3 133,015 - -0.2 133,015 - +0.0 133,018 - -0.3 133,111 + +0.9 133,112 - +0.2 133,117 - -0.5 133,117 - -0.9 133,117 - -0.1 133,117 - +2.1 133,117 - -1.2 133,117 - -2.1 133,145 - +0.9 133,151 + -1.3 133,151 + -1.1 133,152 - -0.9 133,152 - -0.8 133,152 - -0.0 133,159 + -0.0 133,181 - -3.4 133,237 + -2.3 133,238 - +2.1 133,244 + -0.7 133,244 + -2.3 133,315 + -3.1 133,318 + -2.7 133,349 - -1.3 133,488 + EX28DRAFT_4152 0.30 -3.5 133,634 + EX28DRAFT_4152 0.58 -2.9 133,828 + -1.5 133,862 + -1.6 133,864 + +0.2 133,864 + -0.9 133,865 - -2.4 133,865 - -4.0 133,866 + -1.9 133,866 + -1.5 133,866 + -1.5 133,867 - +0.1 133,891 + -2.1 133,927 + -0.5 133,943 - -0.7
Or see this region's nucleotide sequence