Experiment: MS_media with m-arsenite 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_3728 and EX28DRAFT_3729 are separated by 33 nucleotides EX28DRAFT_3729 and EX28DRAFT_3730 are separated by 11 nucleotides EX28DRAFT_3730 and EX28DRAFT_3731 are separated by 123 nucleotides EX28DRAFT_3731 and EX28DRAFT_3732 overlap by 4 nucleotides
EX28DRAFT_3728: EX28DRAFT_3728 - L-aspartate 1-decarboxylase (EC 4.1.1.11), at 403,913 to 404,293
_3728
EX28DRAFT_3729: EX28DRAFT_3729 - pantoate--beta-alanine ligase, at 404,327 to 405,178
_3729
EX28DRAFT_3730: EX28DRAFT_3730 - 3-methyl-2-oxobutanoate hydroxymethyltransferase, at 405,190 to 405,981
_3730
EX28DRAFT_3731: EX28DRAFT_3731 - 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine diphosphokinase, at 406,105 to 406,584
_3731
EX28DRAFT_3732: EX28DRAFT_3732 - poly(A) polymerase, at 406,581 to 407,945
_3732
Position (kb) 405
406 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 404.230 kb on - strand, within EX28DRAFT_3728 at 404.269 kb on + strand at 404.269 kb on + strand at 404.270 kb on - strand at 404.270 kb on - strand at 404.301 kb on - strand at 404.329 kb on - strand at 404.357 kb on + strand at 404.503 kb on + strand, within EX28DRAFT_3729 at 404.504 kb on - strand, within EX28DRAFT_3729 at 404.646 kb on - strand, within EX28DRAFT_3729 at 404.856 kb on + strand, within EX28DRAFT_3729 at 404.856 kb on + strand, within EX28DRAFT_3729 at 404.857 kb on - strand, within EX28DRAFT_3729 at 405.016 kb on + strand, within EX28DRAFT_3729 at 405.017 kb on - strand, within EX28DRAFT_3729 at 405.031 kb on + strand, within EX28DRAFT_3729 at 405.068 kb on - strand, within EX28DRAFT_3729 at 405.110 kb on - strand at 405.110 kb on - strand at 405.116 kb on - strand at 405.243 kb on + strand at 405.243 kb on + strand at 405.244 kb on - strand at 405.245 kb on + strand at 405.246 kb on - strand at 405.246 kb on - strand at 405.414 kb on + strand, within EX28DRAFT_3730 at 405.531 kb on + strand, within EX28DRAFT_3730 at 405.531 kb on + strand, within EX28DRAFT_3730 at 405.531 kb on + strand, within EX28DRAFT_3730 at 405.531 kb on + strand, within EX28DRAFT_3730 at 405.531 kb on + strand, within EX28DRAFT_3730 at 405.534 kb on - strand, within EX28DRAFT_3730 at 405.778 kb on - strand, within EX28DRAFT_3730 at 405.902 kb on + strand at 405.903 kb on - strand at 405.909 kb on - strand at 405.959 kb on - strand at 405.981 kb on + strand at 406.029 kb on - strand at 406.052 kb on + strand at 406.052 kb on + strand at 406.052 kb on + strand at 406.053 kb on - strand at 406.053 kb on - strand at 406.056 kb on + strand at 406.955 kb on - strand, within EX28DRAFT_3732 at 406.955 kb on - strand, within EX28DRAFT_3732 at 406.955 kb on - strand, within EX28DRAFT_3732
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media with m-arsenite 1 mM remove 404,230 - EX28DRAFT_3728 0.83 -3.3 404,269 + -1.8 404,269 + -1.8 404,270 - -0.9 404,270 - -1.8 404,301 - -2.3 404,329 - -1.0 404,357 + -3.4 404,503 + EX28DRAFT_3729 0.21 -1.7 404,504 - EX28DRAFT_3729 0.21 -4.3 404,646 - EX28DRAFT_3729 0.37 -2.6 404,856 + EX28DRAFT_3729 0.62 -4.3 404,856 + EX28DRAFT_3729 0.62 -3.3 404,857 - EX28DRAFT_3729 0.62 -3.2 405,016 + EX28DRAFT_3729 0.81 -2.9 405,017 - EX28DRAFT_3729 0.81 -2.1 405,031 + EX28DRAFT_3729 0.83 -4.1 405,068 - EX28DRAFT_3729 0.87 -3.9 405,110 - -4.2 405,110 - -2.9 405,116 - -4.4 405,243 + -4.1 405,243 + -3.1 405,244 - -2.4 405,245 + -4.4 405,246 - -2.7 405,246 - -0.9 405,414 + EX28DRAFT_3730 0.28 -0.7 405,531 + EX28DRAFT_3730 0.43 -2.3 405,531 + EX28DRAFT_3730 0.43 -2.7 405,531 + EX28DRAFT_3730 0.43 -3.4 405,531 + EX28DRAFT_3730 0.43 -3.5 405,531 + EX28DRAFT_3730 0.43 -0.5 405,534 - EX28DRAFT_3730 0.43 -2.7 405,778 - EX28DRAFT_3730 0.74 -3.9 405,902 + -2.9 405,903 - -3.2 405,909 - -2.0 405,959 - -1.8 405,981 + -1.7 406,029 - -0.5 406,052 + -0.5 406,052 + +0.4 406,052 + -0.6 406,053 - -0.2 406,053 - +0.3 406,056 + -0.3 406,955 - EX28DRAFT_3732 0.27 +0.6 406,955 - EX28DRAFT_3732 0.27 -0.9 406,955 - EX28DRAFT_3732 0.27 -0.2
Or see this region's nucleotide sequence