Experiment: MS_media with m-arsenite 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_3475 and EX28DRAFT_3476 are separated by 19 nucleotides EX28DRAFT_3476 and EX28DRAFT_3477 are separated by 53 nucleotides EX28DRAFT_3477 and EX28DRAFT_3478 are separated by 12 nucleotides EX28DRAFT_3478 and EX28DRAFT_3479 are separated by 108 nucleotides EX28DRAFT_3479 and EX28DRAFT_3480 overlap by 8 nucleotides
EX28DRAFT_3475: EX28DRAFT_3475 - hypothetical protein, at 143,469 to 143,654
_3475
EX28DRAFT_3476: EX28DRAFT_3476 - hypothetical protein, at 143,674 to 143,841
_3476
EX28DRAFT_3477: EX28DRAFT_3477 - Helix-turn-helix domain, at 143,895 to 144,509
_3477
EX28DRAFT_3478: EX28DRAFT_3478 - hypothetical protein, at 144,522 to 144,803
_3478
EX28DRAFT_3479: EX28DRAFT_3479 - hypothetical protein, at 144,912 to 145,295
_3479
EX28DRAFT_3480: EX28DRAFT_3480 - hypothetical protein, at 145,288 to 145,623
_3480
Position (kb)
144
145 Strain fitness (log2 ratio)
-2
-1
0
1 at 143.545 kb on + strand, within EX28DRAFT_3475 at 143.545 kb on + strand, within EX28DRAFT_3475 at 143.789 kb on - strand, within EX28DRAFT_3476 at 143.856 kb on + strand at 143.860 kb on - strand at 143.881 kb on + strand at 144.013 kb on - strand, within EX28DRAFT_3477 at 144.033 kb on - strand, within EX28DRAFT_3477 at 144.116 kb on + strand, within EX28DRAFT_3477 at 144.142 kb on + strand, within EX28DRAFT_3477 at 144.167 kb on + strand, within EX28DRAFT_3477 at 144.173 kb on + strand, within EX28DRAFT_3477 at 144.174 kb on - strand, within EX28DRAFT_3477 at 144.174 kb on - strand, within EX28DRAFT_3477 at 144.192 kb on + strand, within EX28DRAFT_3477 at 144.193 kb on - strand, within EX28DRAFT_3477 at 144.193 kb on - strand, within EX28DRAFT_3477 at 144.220 kb on + strand, within EX28DRAFT_3477 at 144.257 kb on - strand, within EX28DRAFT_3477 at 144.321 kb on - strand, within EX28DRAFT_3477 at 144.340 kb on - strand, within EX28DRAFT_3477 at 144.421 kb on + strand, within EX28DRAFT_3477 at 144.421 kb on + strand, within EX28DRAFT_3477 at 144.475 kb on - strand at 144.539 kb on - strand at 144.572 kb on + strand, within EX28DRAFT_3478 at 144.614 kb on - strand, within EX28DRAFT_3478 at 144.668 kb on - strand, within EX28DRAFT_3478 at 144.704 kb on - strand, within EX28DRAFT_3478 at 144.751 kb on - strand, within EX28DRAFT_3478 at 144.840 kb on + strand at 145.084 kb on + strand, within EX28DRAFT_3479 at 145.100 kb on - strand, within EX28DRAFT_3479 at 145.123 kb on - strand, within EX28DRAFT_3479 at 145.183 kb on + strand, within EX28DRAFT_3479 at 145.183 kb on + strand, within EX28DRAFT_3479 at 145.200 kb on - strand, within EX28DRAFT_3479 at 145.215 kb on + strand, within EX28DRAFT_3479 at 145.215 kb on + strand, within EX28DRAFT_3479 at 145.215 kb on + strand, within EX28DRAFT_3479 at 145.216 kb on - strand, within EX28DRAFT_3479 at 145.217 kb on + strand, within EX28DRAFT_3479 at 145.294 kb on - strand at 145.294 kb on - strand at 145.296 kb on + strand at 145.297 kb on - strand at 145.297 kb on - strand at 145.300 kb on + strand at 145.301 kb on - strand at 145.318 kb on - strand at 145.365 kb on - strand, within EX28DRAFT_3480 at 145.382 kb on + strand, within EX28DRAFT_3480 at 145.528 kb on - strand, within EX28DRAFT_3480 at 145.561 kb on + strand, within EX28DRAFT_3480 at 145.561 kb on + strand, within EX28DRAFT_3480 at 145.561 kb on + strand, within EX28DRAFT_3480 at 145.562 kb on - strand, within EX28DRAFT_3480 at 145.562 kb on - strand, within EX28DRAFT_3480 at 145.562 kb on - strand, within EX28DRAFT_3480 at 145.562 kb on - strand, within EX28DRAFT_3480 at 145.562 kb on - strand, within EX28DRAFT_3480 at 145.562 kb on - strand, within EX28DRAFT_3480 at 145.673 kb on + strand at 145.673 kb on + strand at 145.675 kb on + strand at 145.675 kb on + strand at 145.676 kb on - strand at 145.680 kb on + strand at 145.706 kb on + strand at 145.714 kb on + strand at 145.743 kb on - strand at 145.761 kb on + strand at 145.764 kb on + strand at 145.764 kb on + strand at 145.765 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media with m-arsenite 1 mM remove 143,545 + EX28DRAFT_3475 0.41 -0.3 143,545 + EX28DRAFT_3475 0.41 +0.1 143,789 - EX28DRAFT_3476 0.68 +0.7 143,856 + +0.6 143,860 - -0.1 143,881 + -0.1 144,013 - EX28DRAFT_3477 0.19 +0.4 144,033 - EX28DRAFT_3477 0.22 +0.7 144,116 + EX28DRAFT_3477 0.36 +1.1 144,142 + EX28DRAFT_3477 0.40 -0.2 144,167 + EX28DRAFT_3477 0.44 -0.2 144,173 + EX28DRAFT_3477 0.45 -1.2 144,174 - EX28DRAFT_3477 0.45 +0.8 144,174 - EX28DRAFT_3477 0.45 +0.9 144,192 + EX28DRAFT_3477 0.48 -0.6 144,193 - EX28DRAFT_3477 0.48 +1.0 144,193 - EX28DRAFT_3477 0.48 +0.2 144,220 + EX28DRAFT_3477 0.53 +0.4 144,257 - EX28DRAFT_3477 0.59 +0.4 144,321 - EX28DRAFT_3477 0.69 -2.2 144,340 - EX28DRAFT_3477 0.72 -0.3 144,421 + EX28DRAFT_3477 0.86 -0.7 144,421 + EX28DRAFT_3477 0.86 -0.0 144,475 - +1.1 144,539 - +0.5 144,572 + EX28DRAFT_3478 0.18 -0.2 144,614 - EX28DRAFT_3478 0.33 -0.2 144,668 - EX28DRAFT_3478 0.52 +0.9 144,704 - EX28DRAFT_3478 0.65 -0.4 144,751 - EX28DRAFT_3478 0.81 +0.5 144,840 + +0.0 145,084 + EX28DRAFT_3479 0.45 +0.2 145,100 - EX28DRAFT_3479 0.49 -1.1 145,123 - EX28DRAFT_3479 0.55 +0.2 145,183 + EX28DRAFT_3479 0.71 -1.6 145,183 + EX28DRAFT_3479 0.71 +0.8 145,200 - EX28DRAFT_3479 0.75 +0.5 145,215 + EX28DRAFT_3479 0.79 -0.6 145,215 + EX28DRAFT_3479 0.79 -0.8 145,215 + EX28DRAFT_3479 0.79 +0.3 145,216 - EX28DRAFT_3479 0.79 -0.5 145,217 + EX28DRAFT_3479 0.79 +0.6 145,294 - +1.3 145,294 - +0.2 145,296 + -0.7 145,297 - +0.5 145,297 - +0.2 145,300 + -0.1 145,301 - +0.3 145,318 - +0.9 145,365 - EX28DRAFT_3480 0.23 -0.2 145,382 + EX28DRAFT_3480 0.28 +0.2 145,528 - EX28DRAFT_3480 0.71 -0.2 145,561 + EX28DRAFT_3480 0.81 +0.5 145,561 + EX28DRAFT_3480 0.81 -0.1 145,561 + EX28DRAFT_3480 0.81 +0.3 145,562 - EX28DRAFT_3480 0.82 +0.3 145,562 - EX28DRAFT_3480 0.82 -0.2 145,562 - EX28DRAFT_3480 0.82 -0.2 145,562 - EX28DRAFT_3480 0.82 +1.4 145,562 - EX28DRAFT_3480 0.82 +0.6 145,562 - EX28DRAFT_3480 0.82 -0.8 145,673 + -0.4 145,673 + +0.1 145,675 + +1.0 145,675 + -1.1 145,676 - +0.5 145,680 + +0.2 145,706 + -1.4 145,714 + -1.0 145,743 - -0.5 145,761 + -0.1 145,764 + -0.7 145,764 + -1.7 145,765 - +0.1
Or see this region's nucleotide sequence