Experiment: MS_media with m-arsenite 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_3221 and EX28DRAFT_3222 are separated by 17 nucleotides EX28DRAFT_3222 and EX28DRAFT_3223 are separated by 89 nucleotides EX28DRAFT_3223 and EX28DRAFT_3224 are separated by 287 nucleotides EX28DRAFT_3224 and EX28DRAFT_3225 are separated by 191 nucleotides EX28DRAFT_3225 and EX28DRAFT_3226 are separated by 9 nucleotides EX28DRAFT_3226 and EX28DRAFT_3227 are separated by 223 nucleotides
EX28DRAFT_3221: EX28DRAFT_3221 - Uncharacterized protein conserved in bacteria, at 521,432 to 521,884
_3221
EX28DRAFT_3222: EX28DRAFT_3222 - RNA, at 521,902 to 522,002
_3222
EX28DRAFT_3223: EX28DRAFT_3223 - tRNA, at 522,092 to 522,168
_3223
EX28DRAFT_3224: EX28DRAFT_3224 - argininosuccinate synthase (EC 6.3.4.5), at 522,456 to 523,802
_3224
EX28DRAFT_3225: EX28DRAFT_3225 - tRNA, at 523,994 to 524,080
_3225
EX28DRAFT_3226: EX28DRAFT_3226 - protein translocase, SecG subunit, at 524,090 to 524,422
_3226
EX28DRAFT_3227: EX28DRAFT_3227 - phosphoglucosamine mutase (EC 5.4.2.10), at 524,646 to 525,983
_3227
Position (kb) 522
523
524 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 522.214 kb on + strand at 522.214 kb on + strand at 522.259 kb on + strand at 522.263 kb on - strand at 522.265 kb on - strand at 522.270 kb on + strand at 522.312 kb on - strand at 522.455 kb on + strand at 522.494 kb on + strand at 522.495 kb on - strand at 522.576 kb on + strand at 522.613 kb on - strand, within EX28DRAFT_3224 at 522.613 kb on - strand, within EX28DRAFT_3224 at 522.643 kb on - strand, within EX28DRAFT_3224 at 522.643 kb on - strand, within EX28DRAFT_3224 at 522.751 kb on - strand, within EX28DRAFT_3224 at 522.852 kb on - strand, within EX28DRAFT_3224 at 522.916 kb on - strand, within EX28DRAFT_3224 at 523.005 kb on - strand, within EX28DRAFT_3224 at 523.027 kb on - strand, within EX28DRAFT_3224 at 523.400 kb on + strand, within EX28DRAFT_3224 at 523.401 kb on - strand, within EX28DRAFT_3224 at 523.401 kb on - strand, within EX28DRAFT_3224 at 523.534 kb on + strand, within EX28DRAFT_3224 at 523.563 kb on + strand, within EX28DRAFT_3224 at 523.600 kb on - strand, within EX28DRAFT_3224 at 523.679 kb on + strand at 523.679 kb on + strand at 523.719 kb on + strand at 523.719 kb on + strand at 523.719 kb on + strand at 523.720 kb on - strand at 523.721 kb on + strand at 523.801 kb on - strand at 523.810 kb on + strand at 523.810 kb on + strand at 523.813 kb on - strand at 523.813 kb on - strand at 523.813 kb on - strand at 523.846 kb on + strand at 523.948 kb on - strand at 523.948 kb on - strand at 524.423 kb on - strand at 524.557 kb on - strand at 524.634 kb on + strand at 524.635 kb on - strand at 524.647 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media with m-arsenite 1 mM remove 522,214 + -1.1 522,214 + -0.2 522,259 + -1.5 522,263 - +0.3 522,265 - -0.5 522,270 + -0.0 522,312 - +0.8 522,455 + -0.2 522,494 + -3.6 522,495 - -1.4 522,576 + -1.1 522,613 - EX28DRAFT_3224 0.12 -0.7 522,613 - EX28DRAFT_3224 0.12 -0.5 522,643 - EX28DRAFT_3224 0.14 -2.4 522,643 - EX28DRAFT_3224 0.14 -0.5 522,751 - EX28DRAFT_3224 0.22 -1.8 522,852 - EX28DRAFT_3224 0.29 -1.8 522,916 - EX28DRAFT_3224 0.34 -0.5 523,005 - EX28DRAFT_3224 0.41 -2.5 523,027 - EX28DRAFT_3224 0.42 +0.1 523,400 + EX28DRAFT_3224 0.70 -2.3 523,401 - EX28DRAFT_3224 0.70 -2.9 523,401 - EX28DRAFT_3224 0.70 -0.9 523,534 + EX28DRAFT_3224 0.80 +1.1 523,563 + EX28DRAFT_3224 0.82 -1.1 523,600 - EX28DRAFT_3224 0.85 +1.7 523,679 + +1.1 523,679 + -0.2 523,719 + +2.1 523,719 + -1.1 523,719 + -1.9 523,720 - +0.1 523,721 + +2.1 523,801 - +0.2 523,810 + -0.0 523,810 + +0.3 523,813 - -0.2 523,813 - -0.8 523,813 - -1.1 523,846 + -0.1 523,948 - -0.3 523,948 - -0.2 524,423 - -3.3 524,557 - -3.4 524,634 + +0.6 524,635 - -0.3 524,647 + -1.0
Or see this region's nucleotide sequence